cDNA Clone SAP97 (rat)

• Plasmid: pCMV5-HA SAP97
• Parent Plasmid: pCMV5
• Expressed: HA-SAP97
• Genbank: AM710296.2
• Tag: HA
• Species: Rat

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCCCGGTCCG
GAAGCAAGATACCCAGAGAGCATTGCATCTGTTGGAAGAATATCGGTCGA
AACTAAACCAAACCGAAGACAGACAGCTCAGGAGTTCCATAGAGCGGGTT
ATTAGCATATTTCAGAGCAACCTCTTTCAGGCTTTAATAGACATTCAAGA
ATTTTATGAAGTGACCTTACTTGATAATCCAAAATGTGTGGATCATTCAA
AGCAGTGTGAACCGGTCCAACCCGGGAATCCTTGGGAGAGTGGCAGCCTT
TCAAGTGCTGCTGTGACTTCAGAGAGCCTGCCCGGCGGCCTTAGCCCTCC
AGTAGAGAAATACCGGTATCAGGACGAAGAGGTGCTGCCTTCAGAGCGGA
TTTCCCCGCAAGTCCCAAATGAGGTGCTGGGTCCGGAGCTGGTTCACGTC
TCGGAGAAGAGCCTGTCAGAGATTGAGAATGTCCACGGGTTTGTCTCTCA
CTCTCATATCTCACCCATAAAGGCAAATCCTCCTCCTGTGCTGGTCAACA
CAGACAGCTTGGAGACACCAACTTATGTTAATGGTACTGATGCAGATTAT
GAATATGAGGAAATCACACTTGAAAGGGGAAATTCAGGCCTTGGTTTCAG
CATTGCAGGAGGTACAGACAACCCACACATTGGAGATGACTCAAGTATTT
TCATCACCAAAATTATCACGGGAGGAGCAGCTGCCCAGGATGGAAGATTG
CGGGTAAATGACTGTATACTGAGAGTAAATGAAGCAGATGTTCGTGATGT
AACCCACAGCAAAGCAGTTGAAGCATTGAAAGAAGCTGGATCGATAGTGC
GATTATATGTGAAAAGGCGGAAGCCAGCCTCAGAAAAAATCATGGAAATA
AAACTTATTAAAGGCCCTAAAGGTCTTGGGTTCAGCATTGCTGGAGGTGT
TGGAAACCAGCACATTCCTGGGGATAACAGCATCTATGTAACCAAAATAA
TTGAAGGAGGTGCAGCACACAAGGACGGCAAACTTCAGATTGGAGACAAG
CTTCTAGCAGTGAACAGTGTCTGTTTAGAAGAAGTTACTCATGAAGAAGC
AGTGACTGCCTTAAAGAATACATCTGATTTTGTTTATTTGAAAGTGGCAA
AACCAACAAGTATGTATATAAATGATGGCTATGCACCACCCGACATCACT
AATTCTTCTTCTCAGTCTGTTGACAACCATGTTAGCCCGTCTTCATACTT
GGGCCAGACTCCAGCGTCACCAGCCAGATACTCACCCATTTCTAAAGCAG
TGCTTGGAGATGATGAGATCACTAGGGAACCTAGAAAAGTTGTTCTTCAT
CGTGGCTCAACGGGACTTGGTTTCAACATTGTGGGAGGCGAAGATGGAGA
AGGGATTTTTATCTCTTTCATCCTTGCTGGGGGACCTGCTGACCTAAGCG
GAGAGCTCAGAAAAGGAGATCGCATTATATCGGTAAACAGTGTTGACCTC
AGAGCTGCAAGTCATGAACAAGCAGCTGCCGCATTAAAAAACGCAGGCCA
AGCTGTCACTATTGTCGCACAGTATCGGCCTGAAGAGTACAGTCGTTTTG
AAGCTAAAATTCACGACTTGCGGGAGCAGATGATGAACAGCAGTGTCAGC
TCAGGGTCAGGGTCTCTCCGAACCAGCCAAAAGCGGTCCCTCTATGTCAG
AGCCCTTTTTGATTACGACAAGACTAAAGACAGTGGCCTTCCCAGTCAAG
GGTTGAACTTCAAATTCGGAGATATTCTCCATGTTATCAATGCTTCTGAT
GACGAGTGGTGGCAGGCCAGACAGGTGACCCCAGATGGTGAGAGTGACGA
AGTTGGAGTAATTCCTAGTAAACGCAGAGTTGAGAAGAAAGAACGAGCCC
GATTAAAAACAGTCAAATTCAATTCTAAAACAAGAGGAGATAAAGGGTCA
TTCAATGACAAGCGTAAAAAGAACCTCTTTTCCCGAAAATTTCCCTTCTA
CAAGAACAAGGACCAGAGTGAGCAGGAAACAAGTGATGCTGACCAGCACG
TAACTTCTAATGCCAGCGATAGCGAAAGTAGTTACCGTGGTCAAGAAGAA
TATGTTTTATCGTATGAGCCGGTGAATCAGCAAGAAGTTAATTATACCCG
ACCAGTCATCATATTAGGACCTATGAAAGACAGAGTAAATGATGACTTAA
TCTCAGAATTTCCTGACAAATTTGGATCCTGTGTCCCTCATACAACTAGA
CCAAAGCGTGACTATGAGGTGGATGGACGAGATTACCATTTTGTGACTTC
AAGAGAACAGATGGAGAAAGATATCCAGGAGCATAAATTCATTGAAGCTG
GCCAGTATAACAACCATCTATACGGAACGAGTGTCCAATCTGTGCGAGCA
GTAGCAGAGAAGGGCAAGCATTGTATCCTTGATGTGTCTGGAAATGCCAT
CAAGAGGTTGCAGATTGCACAGCTCTATCCAATATCTACTTTTATTAAAC
CCAAATCCATGGAAAATATCATGGAAATGAATAAACGCCTAACAGACGAA
CAGGCCAGAAAAACGTTTGAGAGAGCAGTGAGACTGGAGCAGGAGTTCAC
TGAGCATTTCACAGCTATTGTCCAGGGAGACACGCTGGAGGACATTTACA
ATCAAGTGAAGCAGATCATAGAAGAGCAGTCTGGTCCTTACATCTGGGTC
CCAGCAAAAGAAAAATTATGAGCGGCCAAGGGCGAATTC

Amino Acid Sequence

MYPYDVPDYAPVRKQDTQRALHLLEEYRSKLNQTEDRQLRSSIERVISIF
QSNLFQALIDIQEFYEVTLLDNPKCVDHSKQCEPVQPGNPWESGSLSSAA
VTSESLPGGLSPPVEKYRYQDEEVLPSERISPQVPNEVLGPELVHVSEKS
LSEIENVHGFVSHSHISPIKANPPPVLVNTDSLETPTYVNGTDADYEYEE
ITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVND
CILRVNEADVRDVTHSKAVEALKEAGSIVRLYVKRRKPASEKIMEIKLIK
GPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAV
NSVCLEEVTHEEAVTALKNTSDFVYLKVAKPTSMYINDGYAPPDITNSSS
QSVDNHVSPSSYLGQTPASPARYSPISKAVLGDDEITREPRKVVLHRGST
GLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAAS
HEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLREQMMNSSVSSGSG
SLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFKFGDILHVINASDDEWW
QARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGSFNDK
RKKNLFSRKFPFYKNKDQSEQETSDADQHVTSNASDSESSYRGQEEYVLS
YEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRD
YEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEK
GKHCILDVSGNAIKRLQIAQLYPISTFIKPKSMENIMEMNKRLTDEQARK
TFERAVRLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKE
KL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4094
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA. This construct is from EcoRI-digested pCR2.1 clone.
• Low Temp: No