cDNA Clone hs PABPC I

• Plasmid: pCMV5-HA hs PABPC I
• Parent Plasmid: pCMV5
• Expressed: HA-hs PABPC I
• Genbank: NM_002568
• Tag: HA
• Species: Human

Sequence of Insert

GTCGACGCCACCATGTACCCATACGATGTGCCAGATTACGCCAACCCCAG
TGCCCCCAGCTACCCCATGGCCTCGCTCTACGTGGGGGACCTCCACCCCG
ACGTGACCGAGGCGATGCTCTACGAGAAGTTCAGCCCGGCCGGGCCCATC
CTCTCCATCCGGGTCTGCAGGGACATGATCACCCGCCGCTCCTTGGGCTA
CGCGTATGTGAACTTCCAGCAGCCGGCGGACGCGGAGCGTGCTTTGGACA
CCATGAATTTTGATGTTATAAAGGGCAAGCCAGTACGCATCATGTGGTCT
CAGCGTGATCCATCACTTCGCAAAAGTGGAGTAGGCAACATATTCATTAA
AAATCTGGACAAATCCATTGATAATAAAGCACTGTATGATACATTTTCTG
CTTTTGGTAACATCCTTTCATGTAAGGTGGTTTGTGATGAAAATGGTTCC
AAGGGCTATGGATTTGTACACTTTGAGACGCAGGAAGCAGCTGAAAGAGC
TATTGAAAAAATGAATGGAATGCTCCTAAATGATCGCAAAGTATTTGTTG
GACGATTTAAGTCTCGTAAAGAACGAGAAGCTGAACTTGGAGCTAGGGCA
AAAGAATTCACCAATGTTTACATCAAGAATTTTGGAGAAGACATGGATGA
TGAGCGCCTTAAGGATCTCTTTGGCAAGTTTGGGCCTGCCTTAAGTGTGA
AAGTAATGACTGATGAAAGTGGAAAATCCAAAGGATTTGGATTTGTAAGC
TTTGAAAGGCATGAAGATGCACAGAAAGCTGTGGATGAGATGAACGGAAA
GGAGCTCAATGGAAAACAAATTTATGTTGGTCGAGCTCAGAAAAAGGTGG
AACGGCAGACGGAACTTAAGCGCAAATTTGAACAGATGAAACAAGATAGG
ATCACCAGATACCAGGGTGTTAATCTTTATGTGAAAAATCTTGATGATGG
TATTGATGATGAACGTCTCCGGAAAGAGTTTTCTCCATTTGGTACAATCA
CTAGTGCAAAGGTTATGATGGAGGGTGGTCGCAGCAAAGGGTTTGGTTTT
GTATGTTTCTCCTCCCCAGAAGAAGCCACTAAAGCAGTTACAGAAATGAA
CGGTAGAATTGTGGCCACAAAGCCATTGTATGTAGCTTTAGCTCAGCGCA
AAGAAGAGCGCCAGGCTCACCTCACTAACCAGTATATGCAGAGAATGGCA
AGTGTACGAGCTGTTCCCAACCCTGTAATCAACCCCTACCAGCCAGCACC
TCCTTCAGGTTACTTCATGGCAGCTATCCCACAGACTCAGAACCGTGCTG
CATACTATCCTCCTAGCCAAATTGCTCAACTAAGACCAAGTCCTCGCTGG
ACTGCTCAGGGTGCCAGACCTCATCCATTCCAAAATATGCCCGGTGCTAT
CCGCCCAGCTGCTCCTAGACCACCATTTAGTACTATGAGACCAGCTTCTT
CACAGGTTCCACGAGTCATGTCAACACAGCGTGTTGCTAACACATCAACA
CAGACAATGGGTCCACGTCCTGCAGCTGCAGCCGCTGCAGCTACTCCTGC
TGTCCGCACCGTTCCACAGTATAAATATGCTGCAGGAGTTCGCAATCCTC
AGCAACATCTTAATGCACAGCCACAAGTTACAATGCAACAGCCTGCTGTT
CATGTACAAGGTCAGGAACCTTTGACTGCTTCCATGTTGGCATCTGCCCC
TCCTCAAGAGCAAAAGCAAATGTTGGGTGAACGGCTGTTTCCTCTTATTC
AAGCCATGCACCCTACTCTTGCTGGTAAAATCACTGGCATGTTGTTGGAG
ATTGATAATTCAGAACTTCTTCATATGCTCGAGTCTCCAGAGTCACTCCG
TTCTAAGGTTGATGAAGCTGTAGCTGTACTACAAGCCCACCAAGCTAAAG
AGGCTGCCCAGAAAGCAGTTAACAGTGCCACCGGTGTTCCAACTGTTTAA
GGATCC

Amino Acid Sequence

MYPYDVPDYANPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIR
VCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDP
SLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYG
FVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFT
NVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERH
EDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRY
QGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFS
SPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRA
VPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQG
ARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMG
PRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQG
QEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNS
ELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4090
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag. The gap in construct DU4068 was filled in to produce this construct.