cDNA Clone DAZAP1-b

• Plasmid: pEGFP-C1-DAZAP1-b T269D T315D
• Parent Plasmid: pEGFPC1
• Expressed: GFP-DAZAP1-b T269D T315D
• Genbank: NM_018959
• Tag: GFP
• Species: Human

Sequence of Insert

ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGT
CGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGG
GCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACC
ACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTA
CGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT
TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGG
CGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGG
ACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAC
GTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAA
GATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACC
AGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC
TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGA
TCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTCCGGACTCAGATCTCGAGCTCAAGCTTCGAAT
TCTGCAGTCGACGGTACCGCGGGCCCGGGATCCAACAACTCGGGCGCCGA
CGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGCACGACCCAAG
AGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTT
ATCATGAAAGATAAAACCACCAACCAGTCTCGAGGCTTTGGGTTTGTCAA
ATTTAAAGACCCAAACTGTGTGGGGACGGTGCTGGCCAGCAGACCGCACA
CGCTAGATGGCCGAAACATCGACCCCAAGCCATGCACACCCCGGGGGATG
CAGCCGGAGAGAACACGGCCGAAGGAAGGATGGCAGAAAGGACCCAGGAG
CGATAACAGTAAATCAAATAAGATATTTGTCGGTGGAATTCCTCACAATT
GTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACG
GAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGG
ATTTATTACTTTCGAGGACGAACAATCAGTGGACCAGGCTGTCAACATGC
ATTTTCACGACATCATGGGCAAAAAAGTGGAAGTTAAACGAGCTGAGCCA
CGGGACAGCAAGAGCCAAGCGCCGGGACAGCCAGGTGCCAGCCAGTGGGG
GAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGCCAGCCCCCGC
CCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGA
CAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCC
GCCACCCCCACCGTTCACCTCCTACATCGTGTCCGACCCTCCTGGAGGCT
TTCCCCCTCCCCAGGGCTTCCCTCAGGGCTACGGTGCCCCGCCACAGTTC
AGTTTTGGCTACGGGCCTCCACCTCCACCGCCAGATCAGTTCGCCCCTCC
GGGGGTTCCTCCTCCACCAGCCGATCCCGGGGCAGCACCTCTGGCTTTCC
CACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCT
CAGCCAGACTTCCCCTATGGTCAGTATGCAGGTTACGGGCAGGACTTGAG
TGGCTTCGGACAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACG
GGGGTCCCTCCGTGCCAGGGTCGGGGGGCCCCCCCGCCGGCGGCAGCGGC
TTTGGACGAGGGCAGAACCACAACGTGCAAGGGTTCCACCCCTACCGACG
CTAGGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSNNSGADEIGKLFVGGLDWSTTQETLRSYFSQYGEVVDCV
IMKDKTTNQSRGFGFVKFKDPNCVGTVLASRPHTLDGRNIDPKPCTPRGM
QPERTRPKEGWQKGPRSDNSKSNKIFVGGIPHNCGETELREYFKKFGVVT
EVVMIYDAEKQRPRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEP
RDSKSQAPGQPGASQWGSRVVPNAANGWAGQPPPTWQQGYGPQGMWVPAG
QAIGGYGPPPAGRGAPPPPPPFTSYIVSDPPGGFPPPQGFPQGYGAPPQF
SFGYGPPPPPPDQFAPPGVPPPPADPGAAPLAFPPPPSQAAPDMSKPPTA
QPDFPYGQYAGYGQDLSGFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSG
FGRGQNHNVQGFHPYRR*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU4085
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GFP.