cDNA Clone DAZAP1-b

• Plasmid: pGEX6P-1-DAZAP1-b T269D T315D
• Parent Plasmid: pGEX6P1
• Expressed: GST-DAZAP1-b T269D T315D
• Genbank: NP_005911
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTT
GACTGGAGCACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGG
AGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCACCAACCAGTCTC
GAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTGGGGACGGTG
CTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATCGACCCCAAGCC
ATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGAT
GGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTC
GGTGGAATTCCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAA
GAAGTTCGGAGTGGTCACGGAGGTAGTCATGATCTATGACGCCGAGAAGC
AGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACGAACAATCAGTG
GACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGA
AGTTAAACGAGCTGAGCCACGGGACAGCAAGAGCCAAGCGCCGGGACAGC
CAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGC
TGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGG
AATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTG
CAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCCTACATCGTG
TCCGACCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCCTCAGGGCTA
CGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGC
CAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCGATCCCGGG
GCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACAT
GAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGCAG
GTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGC
CAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTCGGGGGGCCC
CCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAAG
GGTTCCACCCCTACCGACGCTAGGGATCCCCAGGAATTCCCGGGTCGACT
CGAGCGGCCGCATCGTGACTGACTGACGATCTGCCTCGCGCGTTTTGGGG
AAGGAACAAAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFNNSGADEIGKLFVGGL
DWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTV
LASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNKIFV
GGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQSV
DQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANG
WAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIV
SDPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPADPG
AAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPS
QQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4079
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST tag.