cDNA Clone DAZAP1-b

• Plasmid: pGEX6P-1-DAZAP1-b T315D
• Parent Plasmid: pGEX6P1
• Expressed: GST-DAZAP1-b T315D
• Genbank: NP_005911
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCAACAACT
CGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGC
ACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGT
AGATTGTGTTATCATGAAAGATAAAACCACCAACCAGTCTCGAGGCTTTG
GGTTTGTCAAATTTAAAGACCCAAACTGTGTGGGGACGGTGCTGGCCAGC
AGACCGCACACGCTAGATGGCCGAAACATCGACCCCAAGCCATGCACACC
CCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGATGGCAGAAAG
GACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTCGGTGGAATT
CCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGG
AGTGGTCACGGAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCC
GAGGTTTTGGATTTATTACTTTCGAGGACGAACAATCAGTGGACCAGGCT
GTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGAAGTTAAACG
AGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGCCAGGTGCCA
GCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGC
CAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGT
GCCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAG
GAGCCCCCCCGCCACCCCCACCGTTCACCTCCTACATCGTGTCCACCCCT
CCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCCTCAGGGCTACGGTGCCCC
GCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGCCAGATCAGT
TTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCGATCCCGGGGCAGCACCT
CTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCC
CCCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGCAGGTTACGGGC
AGGACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGCCAGCAGCCT
CCTTCCTACGGGGGTCCCTCCGTGCCAGGGTCGGGGGGCCCCCCCGCCGG
CGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAAGGGTTCCACC
CCTACCGACGCTAGGGATCCCCGGAATTCCCGGGTCGACTCGAGCGGCCG
CATCGTGACTGACTGACGATCTGCCTCGCGCGTTTCCGGGAAGCCCGAGG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSNNSGADEIGKLFVGGLDWS
TTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTVLAS
RPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNKIFVGGI
PHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQSVDQA
VNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANGWAG
QPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIVSTP
PGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPADPGAAP
LAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPSQQP
PSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4078
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST tag.