cDNA Clone hs PABPC I delta 247-292

• Plasmid: pCMV5-HA hs PABPC I delta 247-292
• Parent Plasmid: pCMV5
• Expressed: HA-hs PABP CI delta 247-292
• Genbank: NM_002568
• Tag: HA
• Species: Human

Sequence of Insert

GTCGACGCCACCATGTACCCATACGATGTGCCAGATTACGCCAACCCCAG
TGCCCCCAGCTACCCCATGGCCTCGCTCTACGTGGGGGACCTCCACCCCG
ACGTGACCGAGGCGATGCTCTACGAGAAGTTCAGCCCGGCCGGGCCCATC
CTCTCCATCCGGGTCTGCAGGGACATGATCACCCGCCGCTCCTTGGGCTA
CGCGTATGTGAACTTCCAGCAGCCGGCGGACGCGGAGCGTGCTTTGGACA
CCATGAATTTTGATGTTATAAAGGGCAAGCCAGTACGCATCATGTGGTCT
CAGCGTGATCCATCACTTCGCAAAAGTGGAGTAGGCAACATATTCATTAA
AAATCTGGACAAATCCATTGATAATAAAGCACTGTATGATACATTTTCTG
CTTTTGGTAACATCCTTTCATGTAAGGTGGTTTGTGATGAAAATGGTTCC
AAGGGCTATGGATTTGTACACTTTGAGACGCAGGAAGCAGCTGAAAGAGC
TATTGAAAAAATGAATGGAATGCTCCTAAATGATCGCAAAGTATTTGTTG
GACGATTTAAGTCTCGTAAAGAACGAGAAGCTGAACTTGGAGCTAGGGCA
AAAGAATTCACCAATGTTTACATCAAGAATTTTGGAGAAGACATGGATGA
TGAGCGCCTTAAGGATCTCTTTGGCAAGTTTGGGCCTGCCTTAAGTGTGA
AAGTAATGACTGATGAAAGTGGAAAATCCAAAGGATTTGGATTTGTAAGC
TTTGAAAGGCATGAAGATGCACAGAAAGGTGTTAATCTTTATGTGAAAAA
TCTTGATGATGGTATTGATGATGAACGTCTCCGGAAAGAGTTTTCTCCAT
TTGGTACAATCACTAGTGCAAAGGTTATGATGGAGGGTGGTCGCAGCAAA
GGGTTTGGTTTTGTATGTTTCTCCTCCCCAGAAGAAGCCACTAAAGCAGT
TACAGAAATGAACGGTAGAATTGTGGCCACAAAGCCATTGTATGTAGCTT
TAGCTCAGCGCAAAGAAGAGCGCCAGGCTCACCTCACTAACCAGTATATG
CAGAGAATGGCAAGTGTACGAGCTGTTCCCAACCCTGTAATCAACCCCTA
CCAGCCAGCACCTCCTTCAGGTTACTTCATGGCAGCTATCCCACAGACTC
AGAACCGTGCTGCATACTATCCTCCTAGCCAAATTGCTCAACTAAGACCA
AGTCCTCGCTGGACTGCTCAGGGTGCCAGACCTCATCCATTCCAAAATAT
GCCCGGTGCTATCCGCCCAGCTGCTCCTAGACCACCATTTAGTACTATGA
GACCAGCTTCTTCACAGGTTCCACGAGTCATGTCAACACAGCGTGTTGCT
AACACATCAACACAGACAATGGGTCCACGTCCTGCAGCTGCAGCCGCTGC
AGCTACTCCTGCTGTCCGCACCGTTCCACAGTATAAATATGCTGCAGGAG
TTCGCAATCCTCAGCAACATCTTAATGCACAGCCACAAGTTACAATGCAA
CAGCCTGCTGTTCATGTACAAGGTCAGGAACCTTTGACTGCTTCCATGTT
GGCATCTGCCCCTCCTCAAGAGCAAAAGCAAATGTTGGGTGAACGGCTGT
TTCCTCTTATTCAAGCCATGCACCCTACTCTTGCTGGTAAAATCACTGGC
ATGTTGTTGGAGATTGATAATTCAGAACTTCTTCATATGCTCGAGTCTCC
AGAGTCACTCCGTTCTAAGGTTGATGAAGCTGTAGCTGTACTACAAGCCC
ACCAAGCTAAAGAGGCTGCCCAGAAAGCAGTTAACAGTGCCACCGGTGTT
CCAACTGTTTAAGGATCC

Amino Acid Sequence

MYPYDVPDYANPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIR
VCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDP
SLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYG
FVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFT
NVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERH
EDAQKGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGF
VCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMA
SVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRW
TAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTST
QTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAV
HVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLE
IDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4069
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.