cDNA Clone MEF-DAZAP1-b

• Plasmid: pCMV5-HA MEF-DAZAP1-b T269A T315A
• Parent Plasmid: pCMV5
• Expressed: HA-MEF-DAZAP1-b T269A T315A
• Genbank: NP_005911/NM_018959
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGGGAGGAA
AAAGATTCAGATCCAGCGAATCACCGACGAGCGGAACCGACAGGTGACTT
TCACCAAGCGGAAGTTTGGCCTGATGAAGAAGGCGTATGAGCTGAGCGTG
CTATGTGACTGCGAGATCGCACTCATCATCTTCAACCACTCCAACAAGCT
GTTCCAGTACGCCAGCACCGACATGGACAAGGTGCTGCTCAAGTACACGG
AGTACAATGAGCCACACGAGAGCCGCACCAACGCCGACATCATCGAGACC
CTGAGGAAGAAGGGCTTCAACGGCTGCGACAGTCCCGAGCCCGACGGGGA
GGACTCGCTGGAACAGAGCCCCCTGCTGGAGGACAAGTACCGACGCGCCA
GCGAGGAGCTCGACGGGCTCTTCCGGCGCTATGGGTCAACTGTCCCGGCC
CCCAACTTTGCCATGCCTGTCACGGTGCCCGTGTCCAATCAGAGCTCACT
GCAGTTCAGCAATCCCAGCGGCTCCCTGGTCACCCCTTCCCTGGTGACAT
CATCCCTCACGGACCCGCGGCTCCTGTCCCCCCAGCAGCCAGCACTACAG
AGGAACAGTGTGTCTCCTGGCCTGCCCCAGCGGCCAGCTAGTGCGGGGGC
CATGCTGGGGGGTGACCTGAACAGTGCTAACGGAGCCTGCCCCAGCCCTG
TTGGGCGAGGTTTTGGATTTATTACTTTCGAGGACGAACAATCAGTGGAC
CAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGAAGT
TAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGCCAG
GTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGG
GCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAAT
GTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTGCAG
GAAGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCCTACATCGTGTCC
GCCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCCTCAGGGCTACGG
TGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGCCAG
ATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCGCTCCCGGGGCA
GCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAG
CAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGCAGGTT
ACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGCCAG
CAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTCGGGGGGCCCCCC
CGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAAGGGT
TCCACCCCTACCGACGCTAGGGATCC

Amino Acid Sequence

MYPYDVPDYAGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDC
EIALIIFNHSNKLFQYASTDMDKVLLKYTEYNEPHESRTNADIIETLRKK
GFNGCDSPEPDGEDSLEQSPLLEDKYRRASEELDGLFRRYGSTVPAPNFA
MPVTVPVSNQSSLQFSNPSGSLVTPSLVTSSLTDPRLLSPQQPALQRNSV
SPGLPQRPASAGAMLGGDLNSANGACPSPVGRGFGFITFEDEQSVDQAVN
MHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANGWAGQP
PPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIVSAPPG
GFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPAAPGAAPLA
FPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPSQQPPS
YGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4061
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.