cDNA Clone DAZAP1-b

• Plasmid: pCMV5-HA Dazap1-b T269D T315D
• Parent Plasmid: pCMV5
• Expressed: HA-DAZAP1-b T269D T315D
• Genbank: NM_018959
• Tag: HA
• Species: Human

Sequence of Insert

GTCGACGCCACCATGTACCCATACGATGTGCCAGATTACGCCAACAACTC
GGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGCA
CGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTA
GATTGTGTTATCATGAAAGATAAAACCACCAACCAGTCTCGAGGCTTTGG
GTTTGTCAAATTTAAAGACCCAAACTGTGTGGGGACGGTGCTGGCCAGCA
GACCGCACACGCTAGATGGCCGAAACATCGACCCCAAGCCATGCACACCC
CGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGATGGCAGAAAGG
ACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTCGGTGGAATTC
CTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGA
GTGGTCACGGAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCCG
AGGTTTTGGATTTATTACTTTCGAGGACGAACAATCAGTGGACCAGGCTG
TCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGAAGTTAAACGA
GCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGCCAGGTGCCAG
CCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGCC
AGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTG
CCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGG
AGCCCCCCCGCCACCCCCACCGTTCACCTCCTACATCGTGTCCGACCCTC
CTGGAGGCTTTCCCCCTCCCCAGGGCTTCCCTCAGGGCTACGGTGCCCCG
CCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGCCAGATCAGTT
TGCCCCTCCGGGGGTTCCTCCTCCACCAGCCGATCCCGGGGCAGCACCTC
TGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCC
CCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGCAGGTTACGGGCA
GGACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGCCAGCAGCCTC
CTTCCTACGGGGGTCCCTCCGTGCCAGGGTCGGGGGGCCCCCCCGCCGGC
GGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAAGGGTTCCACCC
CTACCGACGCTAGGCGGCCGCGC

Amino Acid Sequence

MYPYDVPDYANNSGADEIGKLFVGGLDWSTTQETLRSYFSQYGEVVDCVI
MKDKTTNQSRGFGFVKFKDPNCVGTVLASRPHTLDGRNIDPKPCTPRGMQ
PERTRPKEGWQKGPRSDNSKSNKIFVGGIPHNCGETELREYFKKFGVVTE
VVMIYDAEKQRPRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPR
DSKSQAPGQPGASQWGSRVVPNAANGWAGQPPPTWQQGYGPQGMWVPAGQ
AIGGYGPPPAGRGAPPPPPPFTSYIVSDPPGGFPPPQGFPQGYGAPPQFS
FGYGPPPPPPDQFAPPGVPPPPADPGAAPLAFPPPPSQAAPDMSKPPTAQ
PDFPYGQYAGYGQDLSGFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSGF
GRGQNHNVQGFHPYRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4055
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.