cDNA Clone MEKK1

• Synonyms: MAP3K1
• Plasmid: pFastbac GST 6P1 MEKK1 6His
• Parent Plasmid: pFastbac GST
• Expressed: GST-MEKK1 6His
• Genbank: L13103
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCGTGACCGCAGTGCCCGCTGTGTTTTCCAAGCTGGTAACCATGCT
TAATGCTTCTGGCTCCACCCACTTCACCAGGATGCGCCGGCGTCTGATGG
CTATCGCGGATGAGGTAGAAATTGCCGAGGTCATCCAGCTGGGTGTGGAG
GACACTGTGGATGGGCATCAGGACAGCTTACAGGCCGTGGCCCCCACCAG
CTGTCTAGAAAACAGCTCCCTTGAGCACACAGTCCATAGAGAGAAAACTG
GAAAAGGACTAAGTGCTACGAGACTGAGTGCCAGCTCGGAGGACATTTCT
GACAGACTGGCCGGCGTCTCTGTAGGACTTCCCAGCTCAACAACAACAGA
ACAACCAAAGCCAGCGGTTCAAACAAAAGGCAGACCCCACAGTCAGTGTT
TGAACTCCTCCCCTTTGTCTCATGCTCAATTAATGTTCCCAGCACCATCA
GCCCCTTGTTCCTCTGCCCCGTCTGTCCCAGATATTTCTAAGCACAGACC
CCAGGCATTTGTTCCCTGCAAAATACCTTCCGCATCTCCTCAGACACAGC
GCAAGTTCTCTCTACAATTCCAGAGGAACTGCTCTGAACACCGAGACTCA
GACCAGCTCTCCCCAGTCTTCACTCAGTCAAGACCCCCACCCTCCAGTAA
CATACACAGGCCAAAGCCATCCCGACCCGTTCCGGGCAGTACAAGCAAAC
TAGGGGACGCCACAAAAAGTAGCATGACACTTGATCTGGGCAGTGCTTCC
AGGTGTGACGACAGCTTTGGCGGCGGCGGCAACAGTGGCAACGCCGTCAT
ACCCAGCGACGAGACAGTGTTCACGCCGGTGGAGGACAAGTGCAGGTTAG
ATGTGAACACCGAGCTCAACTCCAGCATCGAGGACCTTCTTGAAGCATCC
ATGCCTTCAAGTGACACGACAGTCACTTTCAAGTCCGAAGTCGCCGTCCT
CTCTCCGGAAAAGGCCGAAAATGACGACACCTACAAAGACGACGTCAATC
ATAATCAAAAGTGCAAAGAAAAGATGGAAGCTGAAGAGGAGGAGGCTTTA
GCGATCGCCATGGCGATGTCAGCGTCTCAGGATGCCCTCCCCATCGTCCC
TCAGCTGCAGGTGGAAAATGGAGAAGATATTATCATCATTCAGCAGGACA
CACCAGAAACTCTTCCAGGACATACCAAAGCGAAACAGCCTTACAGAGAA
GACGCTGAGTGGCTGAAAGGCCAGCAGATAGGCCTCGGAGCATTTTCTTC
CTGTTACCAAGCACAGGATGTGGGGACTGGGACTTTAATGGCTGTGAAAC
AGGTGACGTACGTCAGAAACACATCCTCCGAGCAGGAGGAGGTGGTGGAA
GCGTTGAGGGAAGAGATCCGGATGATGGGTCACCTCAACCATCCAAACAT
CATCCGGATGCTGGGGGCCACGTGCGAGAAGAGCAACTACAACCTCTTCA
TTGAGTGGATGGCGGGAGGATCTGTGGCTCACCTCTTGAGTAAATACGGA
GCTTTCAAGGAGTCAGTCGTCATTAACTACACTGAGCAGTTACTGCGTGG
CCTTTCCTATCTCCACGAGAACCAGATCATTCACAGAGACGTCAAAGGTG
CCAACCTGCTCATTGACAGCACCGGTCAGAGGCTGAGAATTGCAGACTTT
GGAGCTGCTGCCAGGTTGGCATCAAAAGGAACCGGTGCAGGAGAGTTCCA
GGGACAGTTACTGGGGACAATTGCATTCATGGCGCCTGAGGTCCTAAGAG
GTCAGCAGTATGGTAGGAGCTGTGATGTATGGAGTGTTGGCTGCGCCATT
ATAGAAATGGCTTGTGCAAAACCACCTTGGAATGCAGAAAAACACTCCAA
TCATCTCGCCTTGATATTTAAGATTGCTAGCGCAACTACTGCACCGTCCA
TCCCGTCACACCTGTCCCCGGGTCTGCGCGACGTGGCCGTGCGCTGCTTA
GAACTTCAGCCTCAGGACCGGCCTCCGTCCAGAGAGCTGCTGAAACATCC
GGTCTTCCGTACCACGTGGCATCATCACCATCACCATTAGGTCGAC

Amino Acid Sequence

GSVTAVPAVFSKLVTMLNASGSTHFTRMRRRLMAIADEVEIAEVIQLGVE
DTVDGHQDSLQAVAPTSCLENSSLEHTVHREKTGKGLSATRLSASSEDIS
DRLAGVSVGLPSSTTTEQPKPAVQTKGRPHSQCLNSSPLSHAQLMFPAPS
APCSSAPSVPDISKHRPQAFVPCKIPSASPQTQRKFSLQFQRNCSEHRDS
DQLSPVFTQSRPPPSSNIHRPKPSRPVPGSTSKLGDATKSSMTLDLGSAS
RCDDSFGGGGNSGNAVIPSDETVFTPVEDKCRLDVNTELNSSIEDLLEAS
MPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQKCKEKMEAEEEEAL
AIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYRE
DAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVE
ALREEIRMMGHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYG
AFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADF
GAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAI
IEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVAVRCL
ELQPQDRPPSRELLKHPVFRTTWHHHHHH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4049
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: His tag added on the C terminus.