cDNA Clone MEF-DAZAP1-b

• Plasmid: pEBG6P-1 MEF-DAZAP1-b T315A
• Parent Plasmid: pEBG6P
• Expressed: GST-MEF-DAZAP1-b T315A
• Genbank: NP_005911/NM_018959
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCGGGAGGAAAAAGATTCAGATCCAGCGAATCACCGACGAG
CGGAACCGACAGGTGACTTTCACCAAGCGGAAGTTTGGCCTGATGAAGAA
GGCGTATGAGCTGAGCGTGCTATGTGACTGCGAGATCGCACTCATCATCT
TCAACCACTCCAACAAGCTGTTCCAGTACGCCAGCACCGACATGGACAAG
GTGCTGCTCAAGTACACGGAGTACAATGAGCCACACGAGAGCCGCACCAA
CGCCGACATCATCGAGACCCTGAGGAAGAAGGGCTTCAACGGCTGCGACA
GTCCCGAGCCCGACGGGGAGGACTCGCTGGAACAGAGCCCCCTGCTGGAG
GACAAGTACCGACGCGCCAGCGAGGAGCTCGACGGGCTCTTCCGGCGCTA
TGGGTCAACTGTCCCGGCCCCCAACTTTGCCATGCCTGTCACGGTGCCCG
TGTCCAATCAGAGCTCACTGCAGTTCAGCAATCCCAGCGGCTCCCTGGTC
ACCCCTTCCCTGGTGACATCATCCCTCACGGACCCGCGGCTCCTGTCCCC
CCAGCAGCCAGCACTACAGAGGAACAGTGTGTCTCCTGGCCTGCCCCAGC
GGCCAGCTAGTGCGGGGGCCATGCTGGGGGGTGACCTGAACAGTGCTAAC
GGAGCCTGCCCCAGCCCTGTTGGGCGAGGTTTTGGATTTATTACTTTCGA
GGACGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCA
TGGGCAAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGC
CAAGCGCCGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCC
CAACGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAG
GATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGC
TATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTT
CACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGG
GCTTCCCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGG
CCTCCACCTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCC
ACCAGCCGCTCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTC
AGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCC
TATGGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGG
CTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGC
CAGGGTCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAG
AACCACAACGTGCAAGGGTTCCACCCCTACCGACGCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSGRKKIQIQRITDE
RNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNHSNKLFQYASTDMDK
VLLKYTEYNEPHESRTNADIIETLRKKGFNGCDSPEPDGEDSLEQSPLLE
DKYRRASEELDGLFRRYGSTVPAPNFAMPVTVPVSNQSSLQFSNPSGSLV
TPSLVTSSLTDPRLLSPQQPALQRNSVSPGLPQRPASAGAMLGGDLNSAN
GACPSPVGRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKS
QAPGQPGASQWGSRVVPNAANGWAGQPPPTWQQGYGPQGMWVPAGQAIGG
YGPPPAGRGAPPPPPPFTSYIVSTPPGGFPPPQGFPQGYGAPPQFSFGYG
PPPPPPDQFAPPGVPPPPAAPGAAPLAFPPPPSQAAPDMSKPPTAQPDFP
YGQYAGYGQDLSGFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQ
NHNVQGFHPYRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4046
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST.