cDNA Clone PBKgamma

• Plasmid: pGEX4T-1-PBKgamma 1-297
• Parent Plasmid: pGEX4T1
• Expressed: GST-PBKgamma 1-297
• Genbank: X80590
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCCACCCGGGACGAGGCACTGCCGG
ACTCTCATTCTGCACAGGACTTCTATGAGAATTATGAGCCCAAAGAGATC
CTGGGCAGGGGCGTTAGCAGTGTGGTCAGGCGATGCATCCACAAGCCCAC
GAGCCAGGAGTACGCCGTGAAGGTCATCGACGTCACCGGTGGAGGCAGCT
TCAGCCCGGAGGAGGTGCGGGAGCTGCGAGAAGCCACGCTGAAGGAGGTG
GACATCCTGCGCAAGGTCTCAGGGCACCCCAACATCATACAGCTGAAGGA
CACTTATGAGACCAACACTTTCTTCTTCTTGGTGTTTGACCTGATGAAGA
GAGGGGAGCTCTTTGACTACCTCACTGAGAAGGTCACCTTGAGTGAGAAG
GAAACCAGAAAGATCATGCGAGCTCTGCTGGAGGTGATCTGCACCTTGCA
CAAACTCAACATCGTGCACCGGGACCTGAAGCCCGAGAACATTCTCTTGG
ATGACAACATGAACATCAAGCTCACAGACTTTGGCTTTTCCTGCCAGCTG
GAGCCGGGAGAGAGGCTGCGAGAGGTCTGCGGGACCCCCAGTTACCTGGC
CCCTGAGATTATCGAGTGCTCCATGAATGAGGACCACCCGGGCTACGGGA
AAGAGGTGGACATGTGGAGCACTGGCGTCATCATGTACACGCTGCTGGCC
GGCTCCCCGCCCTTCTGGCACCGGAAGCAGATGCTGATGCTGAGGATGAT
CATGAGCGGCAACTACCAGTTTGGCTCGCCCGAGTGGGATGATTACTCGG
ACACCGTGAAGGACCTGGTCTCCCGATTCCTGGTGGTGCAACCCCAGAAC
CGCTACACAGCGGAAGAGGCCTTGGCACACCCCTTCTTCCAGCAGTACTT
GGTGGAGGAAGTGCGGTAAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSTRDEALPDSHSAQDFYENYEPKEI
LGRGVSSVVRRCIHKPTSQEYAVKVIDVTGGGSFSPEEVRELREATLKEV
DILRKVSGHPNIIQLKDTYETNTFFFLVFDLMKRGELFDYLTEKVTLSEK
ETRKIMRALLEVICTLHKLNIVHRDLKPENILLDDNMNIKLTDFGFSCQL
EPGERLREVCGTPSYLAPEIIECSMNEDHPGYGKEVDMWSTGVIMYTLLA
GSPPFWHRKQMLMLRMIMSGNYQFGSPEWDDYSDTVKDLVSRFLVVQPQN
RYTAEEALAHPFFQQYLVEEVR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4030
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.