cDNA Clone Aurora A

• Synonyms: AURKA
• Plasmid: pFBHTb Aurora A
• Parent Plasmid: pFastBac HTb
• Expressed: His-Aurora A
• Genbank: BC027464
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGGATCCGACCGATCTAAAGAAA
ACTGCATTTCAGGACCTGTTAAGGCTACAGCTCCAGTTGGAGGTCCAAAA
CGTGTTCTCGTGACTCAGCAATTTCCTTGTCAGAATCCATTACCTGTAAA
TAGTGGCCAGGCTCAGCGGGTCTTGTGTCCTTCAAATTCTTCCCAGCGCG
TTCCTTTGCAAGCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAAT
CAGAAGCAGAAGCAATTGCAGGCAACCAGTGTACCTCATCCTGTCTCCAG
GCCACTGAATAACACCCAAAAGAGCAAGCAGCCCCTGCCATCGGCACCTG
AAAATAATCCTGAGGAGGAACTGGCATCAAAACAGAAAAATGAAGAATCA
AAAAAGAGGCAGTGGGCTTTGGAAGACTTTGAAATTGGTCGCCCTCTGGG
TAAAGGAAAGTTTGGTAATGTTTATTTGGCAAGAGAAAAGCAAAGCAAGT
TTATTCTGGCTCTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGA
GTGGAGCATCAGCTCAGAAGAGAAGTAGAAATACAGTCCCACCTTCGGCA
TCCTAATATTCTTAGACTGTATGGTTATTTCCATGATGCTACCAGAGTCT
ACCTAATTCTGGAATATGCACCACTTGGAACAGTTTATAGAGAACTTCAG
AAACTTTCAAAGTTTGATGAGCAGAGAACTGCTACTTATATAACAGAATT
GGCAAATGCCCTGTCTTACTGTCATTCGAAGAGAGTTATTCATAGAGACA
TTAAGCCAGAGAACTTACTTCTTGGATCAGCTGGAGAGCTTAAAATTGCA
GATTTTGGGTGGTCAGTACATGCTCCATCTTCCAGGAGGACCACTCTCTG
TGGCACCCTGGACTACCTGCCCCCTGAAATGATTGAAGGTCGGATGCATG
ATGAGAAGGTGGATCTCTGGAGCCTTGGAGTTCTTTGCTATGAATTTTTA
GTTGGGAAGCCTCCTTTTGAGGCAAACACATACCAAGAGACCTACAAAAG
AATATCACGGGTTGAATTCACATTCCCTGACTTTGTAACAGAGGGAGCCA
GGGACCTCATTTCAAGACTGTTGAAGCATAATCCCAGCCAGAGGCCAATG
CTCAGAGAAGTACTTGAACACCCCTGGATCACAGCAAATTCATCAAAACC
ATCAAATTGCCAAAACAAAGAATCAGCTAGCAAACAGTCTTAGGTCGACG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSDRSKENCISGPVKATAPVGGPK
RVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQN
QKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEES
KKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAG
VEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQ
KLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIA
DFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFL
VGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPM
LREVLEHPWITANSSKPSNCQNKESASKQS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4028
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag. NB: There is I57V mutation from the EST.