cDNA Clone TAK1-S

• Plasmid: pCMV6P-1 TAK1
• Parent Plasmid: pCMV5 6P1
• Expressed: GST-AK1
• Genbank: NP_003179
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTCTA
CAGCCTCTGCCGCCTCCTCCTCCTCCTCGTCTTCGGCCGGTGAGATGATC
GAAGCCCCTTCCCAGGTCCTCAACTTTGAAGAGATCGACTACAAGGAGAT
CGAGGTGGAAGAGGTTGTTGGAAGAGGAGCCTTTGGAGTTGTTTGCAAAG
CTAAGTGGAGAGCAAAAGATGTTGCTATTAAACAAATAGAAAGTGAATCT
GAGAGGAAAGCGTTTATTGTAGAGCTTCGGCAGTTATCCCGTGTGAACCA
TCCTAATATTGTAAAGCTTTATGGAGCCTGCTTGAATCCAGTGTGTCTTG
TGATGGAATATGCTGAAGGGGGCTCTTTATATAATGTGCTGCATGGTGCT
GAACCATTGCCATATTATACTGCTGCCCACGCAATGAGTTGGTGTTTACA
GTGTTCCCAAGGAGTGGCTTATCTTCACAGCATGCAACCCAAAGCGCTAA
TTCACAGGGACCTGAAACCACCAAACTTACTGCTGGTTGCAGGGGGGACA
GTTCTAAAAATTTGTGATTTTGGTACAGCCTGTGACATTCAGACACACAT
GACCAATAACAAGGGGAGTGCTGCTTGGATGGCACCTGAAGTTTTTGAAG
GTAGTAATTACAGTGAAAAATGTGACGTCTTCAGCTGGGGTATTATTCTT
TGGGAAGTGATAACGCGTCGGAAACCCTTTGATGAGATTGGTGGCCCAGC
TTTCCGAATCATGTGGGCTGTTCATAATGGTACTCGACCACCACTGATAA
AAAATTTACCTAAGCCCATTGAGAGCCTGATGACTCGTTGTTGGTCTAAA
GATCCTTCCCAGCGCCCTTCAATGGAGGAAATTGTGAAAATAATGACTCA
CTTGATGCGGTACTTTCCAGGAGCAGATGAGCCATTACAGTATCCTTGTC
AGTATTCAGATGAAGGACAGAGCAACTCTGCCACCAGTACAGGCTCATTC
ATGGACATTGCTTCTACAAATACGAGTAACAAAAGTGACACTAATATGGA
GCAAGTTCCTGCCACAAATGATACTATTAAGCGCTTAGAATCAAAATTGT
TGAAAAATCAGGCAAAGCAACAGAGTGAATCTGGACGTTTAAGCTTGGGA
GCCTCCCGTGGGAGCAGTGTGGAGAGCTTGCCCCCAACCTCTGAGGGCAA
GAGGATGAGTGCTGACATGTCTGAAATAGAAGCTAGGATCGCCGCAACCA
CAGGCAACGGACAGCCAAGACGTAGATCCATCCAAGACTTGACTGTAACT
GGAACAGAACCTGGTCAGGTGAGCAGTAGGTCATCCAGTCCCAGTGTCAG
AATGATTACTACCTCAGGACCAACCTCAGAAAAGCCAACTCGAAGTCATC
CATGGACCCCTGATGATTCCACAGATACCAATGGATCAGATAACTCCATC
CCAATGGCTTATCTTACACTGGATCACCAACTACAGCCTCTAGCACCGTG
CCCAAACTCCAAAGAATCTATGGCAGTGTTTGAACAGCATTGTAAAATGG
CACAAGAATATATGAAAGTTCAAACAGAAATTGCATTGTTATTACAGAGA
AAGCAAGAACTAGTTGCAGAACTGGACCAGGATGAAAAGGACCAGCAAAA
TACATCTCGCCTGGTACAGGAACATAAAAAGCTTTTAGATGAAAACAAAA
GCCTTTCTACTTACTACCAGCAATGCAAAAAACAACTAGAGGTCATCAGA
AGTCAGCAGCAGAAACGACAAGGCACTTCATGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSTASAASSSSSSSAGEMI
EAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESES
ERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGA
EPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGT
VLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIIL
WEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSK
DPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSF
MDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQSESGRLSLG
ASRGSSVESLPPTSEGKRMSADMSEIEARIAATTGNGQPRRRSIQDLTVT
GTEPGQVSSRSSSPSVRMITTSGPTSEKPTRSHPWTPDDSTDTNGSDNSI
PMAYLTLDHQLQPLAPCPNSKESMAVFEQHCKMAQEYMKVQTEIALLLQR
KQELVAELDQDEKDQQNTSRLVQEHKKLLDENKSLSTYYQQCKKQLEVIR
SQQQKRQGTS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4019
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.