cDNA Clone ELK4

• Plasmid: pGEX6P-1-ELK4 3′ 6His Tag
• Parent Plasmid: pGEX6P1
• Expressed: GST-ELK4-His Tag
• Genbank: AAH63676
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCGACAGTGCTATCACCCTGTGGCAGTTCCTTCTTCAGCTCCTGCAGAAG
CCTCAGAACAAGCACATGATCTGTTGGACCTCTAATGATGGGCAGTTTAA
GCTTTTGCAGGCAGAAGAGGTGGCTCGTCTCTGGGGGATTCGCAAGAACA
AGCCTAACATGAATTATGACAAACTCAGCCGAGCCCTCAGATACTATTAT
GTAAAGAATATCATCAAAAAAGTGAATGGTCAGAAGTTTGTGTACAAGTT
TGTCTCTTATCCAGAGATTTTGAACATGGATCCAATGACAGTGGGCAGGA
TTGAGGGTGACTGTGAAAGTTTAAACTTCAGTGAAGTCAGCAGCAGTTCC
AAAGATGTGGAGAATGGAGGGAAAGATAAACCACCTCAGCCTGGTGCCAA
GACCTCTAGCCGCAATGACTACATACACTCTGGCTTATATTCTTCATTTA
CTCTCAACTCTTTGAACTCCTCCAATGTAAAGCTTTTCAAATTGATAAAG
ACTGAGAATCCAGCCGAGAAACTGGCAGAGAAAAAATCTCCTCAGGAGCC
CACACCATCTGTCATCAAATTTGTCACGACACCTTCCAAAAAGCCACCGG
TTGAACCTGTTGCTGCCACCATTTCAATTGGCCCAAGTATTTCTCCATCT
TCAGAAGAAACTATCCAAGCTTTGGAGACATTGGTTTCCCCAAAACTGCC
TTCCCTGGAAGCCCCAACCTCTGCCTCTAACGTAATGACTGCTTTTGCCA
CCACACCACCCATTTCGTCCATACCCCCTTTGCAGGAACCTCCCAGAACA
CCTTCACCACCACTGAGTTCTCACCCAGACATCGACACAGACATTGATTC
AGTGGCTTCTCAGCCAATGGAACTTCCAGAGAATTTGTCACTGGAGCCTA
AAGACCAGGATTCAGTCTTGCTAGAAAAGGACAAAGTAAATAATTCATCA
AGATCCAAGAAACCCAAAGGGTTAGAACTGGCACCCACCCTTGTGATCAC
GAGCAGTGATCCAAGCCCACTGGGAATACTGAGCCCATCTCTCCCTACAG
CTTCTCTTACACCAGCATTTTTTTCACAGGTAGCTTGCTCGCTCTTTATG
GTGTCACCATTGCTTTCATTTATTTGCCCTTTTAAGCAAATCCAGAATTT
ATACACTCAAGTTTGCTTTCTGTTACTTAGGTTTGTCTTAGAAAGGTTAT
GTGTGACTGTCATGCACCATCACCATCACCATTAA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFDSAITLWQFLLQLLQK
PQNKHMICWTSNDGQFKLLQAEEVARLWGIRKNKPNMNYDKLSRALRYYY
VKNIIKKVNGQKFVYKFVSYPEILNMDPMTVGRIEGDCESLNFSEVSSSS
KDVENGGKDKPPQPGAKTSSRNDYIHSGLYSSFTLNSLNSSNVKLFKLIK
TENPAEKLAEKKSPQEPTPSVIKFVTTPSKKPPVEPVAATISIGPSISPS
SEETIQALETLVSPKLPSLKAPTSASNVMTAFATTPPISSIPPLQEPPRT
PSPPLSSHPDIDTDIDSVASQPMELPENLSLEPKDQDSVLLEKDKVNNSS
RSKKPKGLELAPTLVITSSDPSPLGILSPSLPTASLTPAFFSQVACSLFM
VSPLLSFICPFKQIQNLYTQVCFLLLRFVLERLCVTVMHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4013
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct has an N terminus GST and a C terminus His tag.