cDNA Clone Nedd4-2 (rat)

• Plasmid: pFBHTc Nedd4-2
• Parent Plasmid: pFastbac HTc
• Expressed: His-Nedd4-2
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGGGATCCGGAATTCAAAGGCCT
ACGTCGACGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAGA
AGTCATAAGTCTCGAGTTAAGGGATTTTTGCGATTGAAAATGGCCCATAT
GCCAAAAAATGGAGGTCAAGATGAAGAAAACAGTGACCAGAGGGATGACA
TGGAGCATGGATGGGAAGTTGTTGACTCAAATGACTCGGCTTCTCAGCAC
CAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAAGAAAAAGT
GAACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACTC
AGTGGCACAGACCAAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAAC
ATCAGACAGATCAACCAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCAG
GCACATCAGCGAAGACTTGGAGCCCGAGCCCTCGGAGGGCGGGGATGTCC
CCGAGCCTTGGGAGACCATTTCAGAGGAAGTGAATATCGCTGGAGACTCT
CTCGGTCTGGCTCTGCCCCCACCACCGGCCTCCCCAGGATCTCGGACCAG
CCCTCAGGAGCTGTCAGAGGAACTATGCAGAAGGCTTCAGATCACTCCAG
ACTCCAATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTCA
AGGTTGAGGTCATGCAGTGTCACCGACGCAGTTGCAGAACAGGGCCATCT
ACCACCGCCATCAGTGGCCTATGTACATACCACGCCGGGTCTGCCCTCAG
GCTGGGAAGAAAGAAAAGATGCTAAGGGGCGCACATACTATGTCAATCAT
AACAATCGAACCACAACTTGGACTCGACCTATCATGCAGCTTGCAGAAGA
TGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCTAATCGAGCCTC
AGATCCGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAACTTTATCTGCC
CCGCTGGAGGGTGCCAAGGACTCACCCGTACGTCGGGCTGTGAAAGACAC
CCTTTCCAACCCACAGTCCCCACAGCCATCACCTTACAACTCCCCCAAAC
CACAACACAAAGTCACACAGAGCTTCTTGCCGCCCGGCTGGGAAATGAGG
ATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAACACAAAGACTAC
AACCTGGGAAGATCCACGTTTGAAGTTTCCAGTACATATGCGGTCAAAGA
CATCTTTAAACCCCAATGACCTTGGCCCCCTTCCTCCTGGCTGGGAAGAA
AGAATTCACTTGGATGGCCGAACGTTTTATATTGATCATAATAGCAAAAT
TACTCAGTGGGAAGACCCAAGACTGCAGAACCCAGCTATTACTGGTCCGG
CTGTCCCTTACTCCAGAGAATTTAAGCAGAAATATGACTACTTCAGGAAG
AAATTAAAGAAACCTGCTGATATCCCCAATAGGTTTGAAATGAAACTTCA
CAGAGATAACATATTTGAAGAGTCCTATCGGAGAATTATGTCCGTGAAAA
GACCAGATGTCCTAAAAGCTAGACTGTGGATTGAGTTTGAATCAGAGAAA
GGTCTTGACTATGGGGGTGTGGCCAGAGAATGGTTCTTCTTACTGTCCAA
AGAGATGTTCAACCCCTACTACGGCCTCTTTGAGTACTCTGCCACGGACA
ACTACACCCTTCAGATCAACCCTAATTCAGGCCTCTGTAATGAGGATCAT
TTGTCCTACTTCACTTTTATTGGAAGAGTTGCTGGTCTGGCCGTATTTCA
TGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTACAAGATGATGT
TGGGAAAGCAGATAACCCTGAATGACATGGAATCTGTGGATAGTGAATAT
TACAACTCTTTGAAATGGATCCTGGAGAATGACCCTACTGAGCTGGACCT
CATGTTCTGCATAGACGAAGAAAACTTTGGACAGACATATCAAGTGGATT
TGAAGCCCAATGGGTCAGAAATAATGGTCACAAATGAAAACAAAAGGGAA
TATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGGGTCCAGAAGCA
GATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCCTATTGATTTGA
TTAAAATTTTTGACGAAAATGAGCTGGAGTTGCTCATGTGCGGCCTCGGT
GATGTGGATGTGAATGACTGGAGACAGCATTCTATTTACAAGAACGGCTA
CTGCCCAAACCACCCCGTCATTCAGTGGTTCTGGAAGGCTGTGCTACTCA
TGGACGCCGAAAAGCGTATCCGGTTACTGCAGTTTGTCACAGGGACATCG
CGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCCAATGGTCCTCA
GCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACTGCCCAGAGCTC
ACACATGCTTTAATCGCCTTGACTTACCTCCATATGAAACCTTTGAAGAT
TTACGAGAGAAACTTCTCATGGCCGAGGAAAATGCTCAAGGATTTGAAGG
GGTGGATTAA

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDERPYTFKDFLLRPR
SHKSRVKGFLRLKMAHMPKNGGQDEENSDQRDDMEHGWEVVDSNDSASQH
QEELPPPPLPPGWEEKVNNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNN
IRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDS
LGLALPPPPASPGSRTSPQELSEELCRRLQITPDSNGEQFSSLIQREPSS
RLRSCSVTDAVAEQGHLPPPSVAYVHTTPGLPSGWEERKDAKGRTYYVNH
NNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSA
PLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMR
IAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEE
RIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRK
KLKKPADIPNRFEMKLHRDNIFEESYRRIMSVKRPDVLKARLWIEFESEK
GLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDH
LSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEY
YNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKRE
YIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLG
DVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTS
RVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFED
LREKLLMAEENAQGFEGVD*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4004
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Insect cell expression plasmid with N terminal His tag.