cDNA Clone Pippin

• Plasmid: pCMV5 Pippin 6His
• Parent Plasmid: pGEX6P1
• Expressed: Pippin-His
• Genbank: AB027011
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGTAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCACTTCAG
AGTCGACGTCACCCCCAGTTGTGCCCCCGCTCCACTCCCCCAAGTCCCCA
GTCTGGCCCACCTTCCCCTTCCACAGGGAGGGCAGCAGGGTCTGGGAGCG
GGGTGGTGTCCCACCTCGGGACCTACCCAGCCCTCTGCCCACCAAGCGGA
CCAGGACCTATTCAGCGACAGCCCGGGCCTCAGCTGGCCCCGTGTTCAAG
GGCGTCTGTAAGCAGTTCTCACGCTCACAGGGCCATGGCTTCATCACCCC
CGAGAACGGGTCCGAGGACATCTTCGTACATGTGTCTGACATCGAGGGGG
AGTACGTGCCAGTGGAGGGCGACGAGGTGACCTACAAGATGTGCCCTATC
CCTCCCAAGAACCAGAAGTTCCAGGCCGTGGAGGTGGTGCTCACTCAGCT
GGCCCCCCACACTCCCCACGAGACGTGGTCTGGCCAGGTCGTGGGCTCCC
ATCATCACCATCACCATTAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSTSESTSPPVVPPLHSPKSP
VWPTFPFHREGSRVWERGGVPPRDLPSPLPTKRTRTYSATARASAGPVFK
GVCKQFSRSQGHGFITPENGSEDIFVHVSDIEGEYVPVEGDEVTYKMCPI
PPKNQKFQAVEVVLTQLAPHTPHETWSGQVVGSHHHHHH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4002
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag and C terminal 6His tag.