cDNA Clone Aurora B

• Synonyms: AURKB
• Plasmid: pOPH 6His-PP1 beta + GST-Aurora B
• Parent Plasmid: pOPH
• Expressed: 6His-PP1 beta + GST-Aurora B
• Genbank: PP1 beta BT019744 (human AA), NM_001101722 (rabbit) Aurora B: NM_004217.2 GI:83776599*
• Tag: GST

Sequence of Insert

DNA sequence of rabbit PP1 beta

ATGGCTCATCATCACCATCACCACATGGAAAACCTGTATTTTCAGGGCCATATGGCGGACGGGGAGCTGAACGTGGACAGCCTCATCACCCGCCTGCTGGAGGTACGAGGATGTCGTCCAGGAAAGATTGTGCAAATGACTGAAGCGGAAGTGCGGGGCCTTTGTATCAAGTCTCGGGAGATCTTCCTCAGCCAGCCTATTCTCTTGGAATTGGAAGCACCACTGAAAATTTGTGGAGATATTCATGGACAGTATACAGATTTACTGAGATTATTTGAGTATGGAGGTTTCCCACCAGAAGCCAACTATCTTTTCTTAGGAGATTATGTGGATAGAGGAAAACAGTCTTTGGAAACCATTTGTTTGCTATTGGCTTACAAAATCAAATATCCAGAGAACTTCTTTCTCTTAAGAGGAAACCATGAGTGTGCTAGCATCAATCGCATTTATGGATTCTATGATGAATGCAAGCGAAGGTTTAATATTAAATTGTGGAAGACCTTCACTGACTGCTTTAACTGTCTGCCTATAGCTGCCATTGTGGATGAGAAGATCTTCTGTTGTCATGGAGGACTGTCACCAGACCTACAATCTATGGAGCAGATTCGGAGAATTATGAGACCCACTGATGTCCCTGATACAGGTTTGCTCTGTGATTTACTGTGGTCTGACCCAGACAAGGACGTGCAAGGCTGGGGAGAAAATGACCGTGGTGTTTCCTTCACTTTTGGCGCTGATGTAGTCAGTAAATTTCTGAATCGTCATGATTTAGACCTGATTTGTCGAGCTCATCAGGTGGTGGAAGATGGATATGAATTTTTTGCTAAGCGACAATTGGTAACCCTATTTTCAGCCCCAAATTACTGTGGCGAGTTTGATAATGCTGGTGGAATGATGAGTGTGGATGAGACTTTGATGTGTTCATTTCAGATATTGAAACCATCTGAAAAGAAAGCTAAATACCAGTATGGTGGACTGAATTCTGGACGCCCTGTCACTCCACCTCGAACAGCTAATCCGCCGAAGAAAAGGTGAGGATCCAGATCTAAGCTTGGTACC

GST-Aurora B
AGATCTTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATGG
ATCCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAAC
CCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTG
TATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGG
TTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAA
CACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTG
GGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGT
TTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTG
AAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATG
TTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAAC
CCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGG
ACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGT
ATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATAT
AGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATC
CTCCAAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCTATG
GCCCAGAAGGAGAACTCCTACCCCTGGCCCTACGGCCGACAGACGGCTCC
ATCTGGCCTGAGCACCCTGCCCCAGCGAGTCCTCCGGAAAGAGCCTGTCA
CCCCATCTGCACTTGTCCTCATGAGCCGCTCCAATGTCCAGCCCACAGCT
GCCCCTGGCCAGAAGGTGATGGAGAATAGCAGTGGGACACCCGACATCTT
AACGCGGCACTTCACAATTGATGACTTTGAGATTGGGCGTCCTCTGGGCA
AAGGCAAGTTTGGAAACGTGTACTTGGCTCGGGAGAAGAAAAGCCATTTC
ATCGTGGCGCTCAAGGTCCTCTTCAAGTCCCAGATAGAGAAGGAGGGCGT
GGAGCATCAGCTGCGCAGAGAGATCGAAATCCAGGCCCACCTGCACCATC
CCAACATCCTGCGTCTCTACAACTATTTTTATGACCGGAGGAGGATCTAC
TTGATTCTAGAGTATGCCCCCCGCGGGGAGCTCTACAAGGAGCTGCAGAA
GAGCTGCACATTTGACGAGCAGCGAACAGCCACGATCATGGAGGAGTTGG
CAGATGCTCTAATGTACTGCCATGGGAAGAAGGTGATTCACAGAGACATA
AAGCCAGAAAATCTGCTCTTAGGGCTCAAGGGAGAGCTGAAGATTGCTGA
CTTCGGCTGGTCTGTGCATGCGCCCTCCCTGAGGAGGAAGACAATGTGTG
GCACCCTGGACTACCTGCCCCCAGAGATGATTGAGGGGCGCATGCACAAT
GAGAAGGTGGATCTGTGGTGCATTGGAGTGCTTTGCTATGAGCTGCTGGT
GGGGAACCCACCCTTTGAGAGTGCATCACACAACGAGACCTATCGCCGCA
TCGTCAAGGTGGACCTAAAGTTCCCCGCTTCTGTGCCCACGGGAGCCCAG
GACCTCATCTCCAAACTGCTCAGGCATAACCCCTCGGAACGGCTGCCCCT
GGCCCAGGTCTCAGCCCACCCTTGGGTCCGGGCCAACTCTCGGAGGGTGC
TGCCTCCCTCTGCCCTTCAATCTGTCGCCTGAGCGGCCGCAGATCT

Amino Acid Sequence

6HIS-PP1b

MAHHHHHHMENLYFQGHMADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR

GST-AURORA B

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAQKENSYPWPYGRQTAPS
GLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILT
RHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVE
HQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKS
CTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADF
GWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVG
NPPFESASHNETYRRIVKVDLKFPASVPTGAQDLISKLLRHNPSERLPLA
QVSAHPWVRANSRRVLPPSALQSVA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36775
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_004217.2 CDS:; c885t (silent); t893c (M298T); Both derive from MP clone DU1773 used as template, and match AAV38341.1; Species: PP1: rabbit DNA; human AA seq Aurora B: Human seq