cDNA Clone ANKRD28

• Plasmid: pcDNA5-FRT/TO ANKRD28-GFP
• Parent Plasmid: pcDNA5 FRT/TO C-GFP
• Expressed: ANKRD28-GFP
• Genbank: NM_015199.3 GI:304361755*
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCGTTCCTCAAACTCCGTGACCAGCCATCACTGGTGCAAGC
TATATTTAACGGAGATCCTGATGAAGTTCGAGCACTAATATTTAAGAAAG
AAGATGTTAACTTTCAGGACAATGAAAAGCGAACCCCATTGCACGCCGCA
GCTTACCTTGGAGATGCAGAAATCATTGAACTTCTTATTTTATCTGGAGC
TAGAGTTAATGCCAAAGACAGCAAATGGTTGACACCTTTACACAGAGCAG
TTGCATCTTGTAGTGAGGAAGCAGTTCAGGTACTTTTGAAGCATTCTGCA
GATGTTAATGCTCGAGACAAAAATTGGCAAACCCCTTTACATATAGCTGC
TGCTAATAAAGCTGTAAAGTGTGCTGAAGCTTTGGTACCTCTTCTGAGTA
ATGTAAACGTATCTGATCGAGCAGGGAGGACTGCATTACATCATGCAGCT
TTCAGTGGACATGGTGAGATGGTCAAACTACTCTTGTCTAGAGGTGCCAA
TATTAATGCTTTTGACAAGAAAGATAGGCGTGCTATCCATTGGGCAGCAT
ATATGGGTCACATTGAAGTAGTGAAATTGCTTGTGTCGCATGGAGCTGAA
GTGACATGCAAGGATAAAAAGTCTTATACACCTCTTCATGCAGCAGCCTC
TAGTGGAATGATCAGCGTAGTCAAGTACCTTCTAGATCTTGGAGTTGATA
TGAATGAACCAAATGCCTATGGAAATACACCTCTTCATGTAGCCTGCTAT
AATGGACAAGATGTTGTAGTGAATGAACTTATAGACTGTGGTGCTATTGT
GAATCAAAAGAATGAAAAAGGATTTACTCCTTTGCACTTTGCTGCTGCAT
CAACACATGGAGCATTGTGTTTAGAGCTTCTAGTTGGCAATGGGGCCGAT
GTCAATATGAAGAGTAAAGATGGGAAAACCCCACTACACATGACTGCTCT
CCACGGTAGATTCTCCCGATCACAAACCATTATCCAGAGTGGAGCTGTAA
TCGACTGTGAGGATAAGAATGGAAATACCCCTTTGCACATAGCAGCACGG
TATGGCCATGAGCTGCTGATCAACACTCTTATTACAAGTGGTGCTGACAC
TGCAAAGCGTGGCATACATGGAATGTTCCCCCTCCATTTGGCAGCCTTAA
GCGGCTTTTCAGATTGCTGCAGAAAACTTCTTTCTTCAGGATTTGATATA
GATACCCCAGATGATTTTGGCAGGACTTGTCTACATGCAGCTGCAGCTGG
AGGGAATTTGGAGTGCCTAAACCTTCTGCTGAATACTGGTGCAGACTTTA
ATAAAAAGGACAAATTTGGGAGATCTCCACTGCACTACGCTGCTGCCAAC
TGCAATTACCAGTGCCTGTTTGCTCTTGTGGGATCAGGAGCAAGTGTGAA
TGACCTTGATGAAAGAGGCTGCACACCCCTGCACTATGCAGCTACATCAG
ACACAGATGGCAAGTGCCTGGAATACTTATTAAGAAACGATGCAAATCCA
GGGATTCGTGATAAGCAAGGATACAACGCAGTTCATTATTCAGCTGCTTA
TGGTCACCGTCTATGTCTTCAGCTGATTGCAAGTGAAACTCCTCTAGATG
TTTTAATGGAAACCTCAGGAACAGACATGCTGAGTGATTCAGATAATAGA
GCAACAATAAGCCCTTTACACTTGGCTGCCTATCATGGTCACCATCAAGC
ACTGGAAGTGTTGGTACAGTCTTTGTTAGATCTTGATGTCAGAAATAGTA
GTGGAAGAACACCCCTAGATCTTGCAGCTTTTAAGGGCCATGTTGAATGT
GTGGATGTACTCATTAATCAGGGAGCCTCAATCTTAGTAAAAGATTACAT
TTTGAAGAGGACACCTATTCATGCAGCAGCAACAAATGGTCATTCAGAAT
GCTTACGGCTATTAATAGGAAATGCAGAACCACAGAATGCAGTGGATATT
CAAGATGGAAATGGACAGACGCCTCTGATGCTATCTGTTCTCAACGGGCA
CACAGACTGTGTTTACTCATTGCTGAACAAAGGAGCAAATGTAGATGCCA
AAGATAAGTGGGGAAGGACAGCGTTGCATAGAGGGGCAGTTACAGGCCAT
GAAGAATGTGTAGATGCATTACTTCAACATGGTGCTAAGTGCTTACTTCG
GGATAGCAGGGGCCGGACGCCTATACACCTGTCTGCTGCCTGTGGACACA
TTGGTGTTCTTGGAGCCCTTTTGCAGTCAGCAGCATCTATGGATGCAAAT
CCAGCCACAGCAGACAATCATGGATATACGGCACTTCACTGGGCTTGCTA
CAATGGTCACGAGACATGTGTAGAACTGCTTTTAGAACAGGAAGTTTTCC
AGAAAACGGAAGGAAATGCTTTTAGTCCATTGCATTGTGCCGTGATAAAT
GACAACGAAGGTGCTGCTGAGATGTTAATTGATACATTAGGTGCCAGCAT
TGTGAACGCCACAGATTCAAAAGGAAGAACTCCTCTCCATGCAGCCGCCT
TCACAGACCATGTAGAGTGTTTACAGCTGCTGCTCAGCCATAATGCTCAA
GTCAATTCTGTGGACTCTACAGGGAAAACACCTCTTATGATGGCTGCAGA
AAATGGACAAACAAATACAGTTGAGATGCTGGTTAGCAGTGCTAGTGCAG
AACTGACTTTACAAGATAACAGTAAAAATACTGCCCTCCATTTGGCTTGT
AGCAAGGGTCATGAAACTAGTGCCTTGTTAATACTGGAAAAGATAACAGA
TAGAAACCTCATCAATGCAACCAACGCAGCCTTGCAAACACCTCTGCATG
TTGCTGCCCGAAATGGGCTAACAATGGTGGTTCAGGAACTTTTGGGAAAA
GGAGCAAGTGTGCTTGCAGTAGATGAAAATGGCTATACCCCAGCTTTGGC
CTGTGCTCCCAATAAGGATGTGGCTGATTGCCTGGCTCTCATTTTGGCCA
CCATGATGCCTGTCTCATCAAGTAGTCCTTTATCATCCTTAACATTCAAT
GCCATTAACCGTTATACCAACACCTCAAAAACAGTCAGCTTTGAAGCTTT
GCCCATCATGAGGAATGAACCTAGCTCCTATTGCAGTTTCAATAACATTG
GAGGGGAACAGGAGTACTTATACACTGACGTGGATGAGCTCAACGACTCC
GATTCTGAGACCTACGCGGCCGC

Amino Acid Sequence

MAFLKLRDQPSLVQAIFNGDPDEVRALIFKKEDVNFQDNEKRTPLHAAAY
LGDAEIIELLILSGARVNAKDSKWLTPLHRAVASCSEEAVQVLLKHSADV
NARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFS
GHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVT
CKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNG
QDVVVNELIDCGAIVNQKNEKGFTPLHFAAASTHGALCLELLVGNGADVN
MKSKDGKTPLHMTALHGRFSRSQTIIQSGAVIDCEDKNGNTPLHIAARYG
HELLINTLITSGADTAKRGIHGMFPLHLAALSGFSDCCRKLLSSGFDIDT
PDDFGRTCLHAAAAGGNLECLNLLLNTGADFNKKDKFGRSPLHYAAANCN
YQCLFALVGSGASVNDLDERGCTPLHYAATSDTDGKCLEYLLRNDANPGI
RDKQGYNAVHYSAAYGHRLCLQLIASETPLDVLMETSGTDMLSDSDNRAT
ISPLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVD
VLINQGASILVKDYILKRTPIHAAATNGHSECLRLLIGNAEPQNAVDIQD
GNGQTPLMLSVLNGHTDCVYSLLNKGANVDAKDKWGRTALHRGAVTGHEE
CVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSAASMDANPA
TADNHGYTALHWACYNGHETCVELLLEQEVFQKTEGNAFSPLHCAVINDN
EGAAEMLIDTLGASIVNATDSKGRTPLHAAAFTDHVECLQLLLSHNAQVN
SVDSTGKTPLMMAAENGQTNTVEMLVSSASAELTLQDNSKNTALHLACSK
GHETSALLILEKITDRNLINATNAALQTPLHVAARNGLTMVVQELLGKGA
SVLAVDENGYTPALACAPNKDVADCLALILATMMPVSSSSPLSSLTFNAI
NRYTNTSKTVSFEALPIMRNEPSSYCSFNNIGGEQEYLYTDVDELNDSDS
ETYAAAMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYV
QERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEY
NYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPV
LLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36760
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *With silent change c1500t to kill internal BamH1 site