cDNA Clone DENND4B

• Plasmid: pcDNA5-FRT/TO-GFP DENND4B
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP DENND4B
• Genbank: NM_014856.2 GI:148922919
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTATGGCGGAGGAGCGGCCCCCCCGGCTGGTGGATTACTTCGTGGT
AGCTGGGCTTGCAGGGAACGGAGCACCCATCCCTGAGGAAACGTGGGTTC
CTGAACCCAGTGGGCCCCTGCGCCCTCCCCGGCCAGCTGAGCCCATCACA
GATGTGGCAGTCATCGCTAGGGCACTGGGCGAGGAAGTGCCCCAGGGCTA
CACATGCATCCAGGCTTCTGCTGGGGGCCACCCCTTGGAACTCAGTGCTG
GACTTCTGGGTGGAACTCAACCCGTCATCTGCTACCGCAGGGGCCGTGAC
AAGCCCCCCCTCGTTGAGCTGGGGGTGTTGTATGAGGGGAAGGAACGTCC
CAAGCCTGGCTTCCAAGTGCTTGACACGACACCCTACAGCCACTCAGCAA
ACCTGGCCCCTCCAGGCCCCGGGCACCCCCGCACCTACCTCACTTACCGG
CGGGCAGCAGAGGGGGCAGGGCTGCATGCCCTGGGCATCACTGACCTCTG
CCTGGTGCTGCCCAGTAAGGGCGAGGGCACTCCTCATACTTACTGCCGGC
TGCCCCGCAACCTCAACCCTGGCATGTGGGGCCCAGCAGTGTACCTGTGC
TATAAGGTGGGCCTGGCGAAGGCCAACACGCTGGTGTACGAGGCAGAGCT
GCTGGGCCGCTACCCGGAGGAGGACAATGAGGCGTTCCCGCTGCCCGAGT
CAGTGCCCGTCTTCTGCCTGCCCATGGGGGCCACTATCGAGTGCTGGCCT
GCCCAGACCAAGTACCCCGTGCCCGTCTTCTCCACCTTTGTGCTCACGGG
TGCAGCTGGTGATAAGGTGTATGGTGCCGCCCTGCAGTTCTACGAGGCGT
TCCCAAGGGCCAGGCTATCAGAGCGACAGGCACGGGCACTGGGCCTGCTG
AGCGCCGTGGAGCGGGGTCGGGCACTGGGGGGCAGAGCTGTGCGCAGCCG
GCGTGCCATCGCTGTGCTGTCCCGCTGGCCTGCCTTCCCTGCCTTCCGCG
CCTTCCTCACCTTCCTTTACCGCTACTCCGTCTCAGGCCCCCACCGCCTA
CCCTTGGAAGCGCACATCTCCCACTTCATTCACAACGTTCCCTTCCCTTC
CCCACAGAGACCCCGCATCCTAGTGCAGATGTCTCCCTATGACAACTTGC
TCCTCTGTCAGCCTGTATCCTCACCCCTGCCCCTCAGTGGTGCCAGCTTC
CTGCAGCTGCTGCAGAGCCTGGGCCCTGAGCTGGCTATCACACTGCTGCT
GGCTGTGCTCACAGAGCACAAGCTGCTAGTCCACTCGCTGCGGCCAGACC
TGCTCACCAGCGTCTGTGAGGCCCTCGTCTCGATGATCTTCCCACTGCAC
TGGCAGTGCCCCTACATTCCTCTGTGCCCGCTGGTGCTGGCAGATGTGCT
GAGTGCCCCAGTGCCCTTCATTGTGGGTATCCACTCCAGCTACTTTGATC
TGCATGACCCGCCTGCTGATGTCATCTGTGTAGACCTTGATACCAACACG
CTCTTCCAGACTGAGGAAAAGAAGCTCCTCTCCCCTCGGACCCTGCCCCG
CAGACCCTACAAGGTTCTGCTGGCCACACTGACAAACCTGTACCAGCAGC
TGGACCAGACATACACTGGACCTGAGGAGGAAGCATCCCTGGAGTTCCTA
CTGACAGACTACGAGGCAGTGTGTGGCCGCAGGGCCCGGCTGGAGCGCGA
AGTCCAAGGAGCCTTCCTCCGCTTCATGGCCTGTCTGCTCAAGGGCTACC
GGGTCTTCCTGCGCCCACTCACCCAGGCCCCCTCCGAGGGAGCTCGTGAT
GTTGACAACCTTTTCTTCCTGCAGGGCTTCCTCAAATCCCGGGAACGCTC
CAGCCACAAACTTTACTCTCAGCTGCTGCACACACAGATGTTCTCACAGT
TCATTGAGGAGTGCTCTTTTGGCTCTGCTCGCCATGCTGCCCTTGAATTC
TTTGACTCTTGTGTTGAAAAGGTCCACCCAGAGCAGGAGAAGCCTGAGCC
GACACCCTTAGTGGAGCTAGAGGAGCTGTCAGGAAGTGAGCTCACTGTCT
TTATCACACCTCCCGAGGAGCCTGCCTTACCAGAGGGCAGTGAATCCACT
CCCCAGTACTGCTATGATGGATTCCCAGAGCTACGGGCTGAGTTGTTTGA
GTCTCTTCAAGAGCAACCTGGGGCCCTGCCTGTGCCAGGCCCTTCCCGTA
GCGCCCCCAGCAGTCCTGCTCCTCGCCGTACCAAACAGGAGATGAAAGTT
GCACAGCGGATGGCACAGAAGTCAGCAGCTGTGCCTGAGCTGTGGGCCCG
GTGCCTGCTGGGGCACTGCTATGGGCTGTGGTTCCTGTGTCTGCCTGCCT
ATGTGCGGTCGGCACCCTCCCGAGTGCAGGCACTGCACACAGCCTACCAT
GTGCTGCGCCAGATGGAGAGCGGCAAGGTGGTGCTCCCTGATGAGGTGTG
TTACCGGGTACTGATGCAGCTCTGCTCACACTATGGGCAGCCTGTGCTGT
CTGTGCGGGTCATGCTGGAGATGCGTCAGGCAGGCATTGTGCCCAACACC
ATCACCTATGGCTACTACAATAAGGCTGTGTTGGAAAGCAAGTGGCCGTC
TGGCACACCAGGTGGGCGTCTGCGCTGGGCCAAGCTCCGGAATGTTGTCC
TGGGGGCTGCTCAGTTCCGCCAGCCCTTGAGAGAACGGCAACAGCAGCAG
CAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGGAGCAGGTGTCAGC
ACATCAAGAGGCAGGCAGCTCCCAGGCAGAGCCCTATTTGGAGCGCCCTT
CCCCTACTCGCCCTCTTCAGCGCCAGACTACTTGGGCTGGGCGAAGTCTG
AGAGACCCAGCCTCACCCCCTGGACGCCTGGTGAAGAGTGGTAGCCTGGG
CAGTGCCCGAGGGGCACAGCCCACTGTGGAGGCCGGTGTGGCCCACATGA
TAGAGGCCTTGGGGGTCCTGGAACCCCGGGGATCACCTGTGCCCTGGCAC
GATGGAAGTCTCTCAGACCTGAGCCTGACAGGGGAGGAGCCGCTCCCTGG
AGGCAGCCCAGGGGGCTCAGGCTCAGCCCTGAGTGCCCAGTCCACTGAGG
CCCTGGAAGGGCTAAGTGGGCGGGGACCCAAGGCTGGTGGGCGACAGGAT
GAGGCAGGCACCCCCCGACGAGGGCTGGGTGCCCGCCTCCAACAGCTGCT
CACTCCTTCCCGCCACTCCCCTGCCTCCCGCATTCCCCCACCTGAGCTGC
CTCCTGACCTGCCACCCCCAGCCCGCCGCAGCCCCATGGACAGTCTTCTG
CACCCCCGGGAGCGCCCTGGATCCACTGCCTCCGAGAGCTCAGCCTCTCT
GGGCAGTGAGTGGGACCTCTCAGAATCTTCTCTCAGCAACCTGAGTCTTC
GCCGTTCCTCAGAGCGCCTCAGTGACACCCCTGGATCCTTCCAGTCACCT
TCCCTGGAAATTCTGCTGTCCAGCTGCTCCCTGTGCCGTGCCTGTGATTC
GCTGGTGTATGATGAGGAAATCATGGCTGGCTGGGCACCTGATGACTCTA
ACCTCAACACAACCTGCCCCTTCTGCGCCTGCCCCTTTGTGCCCCTGCTC
AGTGTCCAGACCCTTGATTCCCGGCCCAGTGTCCCCAGCCCCAAATCTGC
TGGTGCCAGTGGCAGCAAAGATGCTCCTGTCCCTGGTGGTCCTGGCCCTG
TGCTCAGTGACCGAAGGCTCTGCCTTGCTCTGGATGAGCCCCAGCTCTGC
AACGGGCACATGGGGGGAGCCTCCCGGCGGGTTGAGAGTGGGGCATGGGC
ATACCTGAGCCCCCTGGTGCTGCGTAAGGAGCTGGAGTCGCTGGTAGAGA
ACGAGGGCAGTGAGGTGCTGGCGTTGCCTGAACTGCCCTCTGCCCACCCC
ATCATCTTCTGGAACCTTTTGTGGTATTTCCAACGGCTACGCCTGCCCAG
TATTCTACCAGGCCTGGTGCTGGCCTCCTGTGATGGGCCTTCGCACTCCC
AGGCCCCATCTCCTTGGCTAACCCCTGATCCAGCCTCTGTTCAGGTACGG
CTGCTGTGGGATGTACTGACCCCTGACCCCAATAGCTGCCCACCTCTCTA
TGTGCTCTGGAGGGTCCACAGCCAGATCCCCCAGCGGGTGGTATGGCCAG
GCCCTGTACCTGCATCCCTTAGTTTGGCACTGTTGGAGTCAGTGCTGCGC
CATGTTGGACTCAATGAAGTGCACAAGGCTGTGGGGCTCCTGCTGGAAAC
TCTAGGGCCCCCACCCACTGGCCTGCACCTGCAGAGGGGAATCTACCGTG
AGATATTATTCCTGACAATGGCTGCTCTGGGCAAGGACCACGTGGACATA
GTGGCCTTCGATAAGAAGTACAAGTCTGCCTTTAACAAGCTGGCCAGCAG
CATGGGCAAGGAGGAGCTGAGGCACCGGCGGGCGCAGATGCCCACTCCCA
AGGCCATTGACTGCCGAAAATGTTTTGGAGCACCTCCAGAATGCTAGCGG
CCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAEERP
PRLVDYFVVAGLAGNGAPIPEETWVPEPSGPLRPPRPAEPITDVAVIARA
LGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELG
VLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGL
HALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAVYLCYKVGLAKA
NTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATIECWPAQTKYPVP
VFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRA
LGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISH
FIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLG
PELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPL
CPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKK
LLSPRTLPRRPYKVLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVC
GRRARLEREVQGAFLRFMACLLKGYRVFLRPLTQAPSEGARDVDNLFFLQ
GFLKSRERSSHKLYSQLLHTQMFSQFIEECSFGSARHAALEFFDSCVEKV
HPEQEKPEPTPLVELEELSGSELTVFITPPEEPALPEGSESTPQYCYDGF
PELRAELFESLQEQPGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKS
AAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVQALHTAYHVLRQMESG
KVVLPDEVCYRVLMQLCSHYGQPVLSVRVMLEMRQAGIVPNTITYGYYNK
AVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLRERQQQQQQQQQQQQ
QQQQEQVSAHQEAGSSQAEPYLERPSPTRPLQRQTTWAGRSLRDPASPPG
RLVKSGSLGSARGAQPTVEAGVAHMIEALGVLEPRGSPVPWHDGSLSDLS
LTGEEPLPGGSPGGSGSALSAQSTEALEGLSGRGPKAGGRQDEAGTPRRG
LGARLQQLLTPSRHSPASRIPPPELPPDLPPPARRSPMDSLLHPRERPGS
TASESSASLGSEWDLSESSLSNLSLRRSSERLSDTPGSFQSPSLEILLSS
CSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSR
PSVPSPKSAGASGSKDAPVPGGPGPVLSDRRLCLALDEPQLCNGHMGGAS
RRVESGAWAYLSPLVLRKELESLVENEGSEVLALPELPSAHPIIFWNLLW
YFQRLRLPSILPGLVLASCDGPSHSQAPSPWLTPDPASVQVRLLWDVLTP
DPNSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVH
KAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYK
SAFNKLASSMGKEELRHRRAQMPTPKAIDCRKCFGAPPEC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36735
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee