cDNA Clone DENND4A

• Plasmid: pcDNA5-FRT/TO-GFP DENND4A (Q05C90)
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP DENND4A
• Genbank: BC028057.1 (Q05C90)*
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCGATGATTGAAGACAAGGGGCCTCGTGTTGCTGACTACTTTGT
TGTAGCAGGATTAACTGATGTTTCAAAGCCTCTAGAAGAAGAAATTCACT
TCAATGATGCTTGTCATAAAGTAGCTAAACCAAAAGAACCTATTACAGAT
GTTTCAGTTATTATCAAATCTCTTGGGGAGGAAGTCCCACAGGATTATAT
CTGTATTGATGTTACCCCAACTGGATTGTCAGCTGATCTTAATAATGGAA
GTCTTGTGGGGCCACAGATTTACCTTTGCTATAGAAGAGGAAGAGATAAG
CCTCCACTTACAGATCTGGGGGTTTTATATGACTGGAAAGAAAGATTGAA
ACAGGGTTGTGAAATTATTCAGAGTACTCCCTATGGGCGCCCCGCAAATA
TTAGTGGGAGTACCTCATCACAAAGAATTTATATCACTTACCGAAGAGCC
TCTGAAAACATGACTCAGAATACGTTGGCTGTCACTGACATATGTATTAT
TATACCCAGTAAAGGAGAAAGCCCACCACACACGTTCTGCAAAGTCGACA
AGAATCTCAATAACAGTATGTGGGGATCAGCAGTATACTTGTGTTATAAA
AAATCGGTGGCAAAGACGAACACTGTATCTTACAAAGCTGGCCTAATTTG
TAGATATCCTCAAGAAGATTATGAGTCATTCTCACTACCGGAATCTGTTC
CTCTATTTTGTTTACCAATGGGTGCAACCATTGAATGTTGGCCATCAAAT
AGCAAATACCCTCTGCCTGTATTTTCTACTTTTGTTTTAACTGGAGCCTC
AGCTGAAAAGGTTTATGGTGCTGCTATTCAGTTTTATGAACCATACTCTG
AGGAGAATCTCACAGAAAAGCAGAGACTTCTTTTGGGTTTAACATCAGCA
GATGGGAAGTCTGATAGTTCCAAAACAATTCATACTAACAAATGCATCTG
TCTTCTTTCTCACTGGCCTTTTTTTGATGCATTCAGGAAGTTTCTGACTT
TTCTGTATCGTTATTCCATCTCTGGGCCTCATGTCCTTCCAATTGAGAAG
CATATTTCTCATTTTATGCATAAAGTTCCTTTTCCATCTCCTCAGAGACC
ACGGATTTTAGTTCAGTTATCACCACATGATAATCTGATTCTCAGTCAGC
CTGTGTCTTCACCTCTTCCACTAAGTGGTGGCAAGTTTTCAACACTACTG
CAGAATTTAGGCCCTGAAAATGCTGTGACACTACTGGTGTTTGCAGTAAC
AGAACATAAAATTCTTATCCATTCCCTACGGCCATCCGTGCTTACTAGTG
TGACAGAAGCATTAGTGTCTATGATTTTCCCTTTCCACTGGCCATGCCCG
TATGTTCCTCTCTGCCCACTGGCTTTAGCAGATGTCTTGAGTGCACCATG
TCCATTCATAGTAGGGATTGATTCAAGATACTTTGATCTCTATGATCCTC
CACCAGATGTCAGTTGTGTTGATGTAGATACCAACACTATTTCTCAAATT
GGTGACAAGAAAAATGTAGCCTGGAAGATACTTCCAAAGAAGCCATGTAA
AAATCTGATGAACACACTGAATAATTTGCATCAGCAATTGGCAAAATTGC
AGCAGAGACCGAGAGATGATGGACTCATGGATTTAGCAATAAATGACTAT
GATTTTAATTCTGGAAAGAGGTTGCACATGATAGATTTGGAAATCCAAGA
GGCATTTTTGTTTTTCATGGCCTCTATTTTAAAAGGTTACAGATCATACT
TAAGGCCAATAACACAAGCCCCATCTGAGACAGCCACAGATGCAGCCTCT
CTCTTTGCCCTACAAGCTTTCTTAAGAAGCCGGGACCGGTCACATCAAAA
ATTCTATAACATGATGACCAAAACACAAATGTTTATTCGCTTCATTGAAG
AATGTTCTTTTGTTAGTGACAAAGATGCAAGCCTGGCATTTTTTGATGAT
TGTGTAGATAAAGTGGATATGGACAAGAGCGGTGAAGTACGACTTATAGA
ACTGGATGAATCTTTCAAAAGTGAACACACTGTTTTTGTAACACCACCTG
AGATCCCCCACCTACCCAATGGTGAAGAACCCCCTTTACAGTACAGCTAT
AATGGATTTCCAGTTCTTAGGAACAATTTATTTGAAAGACCTGAAGGATT
TCTACAAGCAAAGAAGAACAAATTGCCTTCAAAATCAAGTAGTCCTAATA
GCCCTTTGCCCATGTTCAGAAGAACAAAACAGGAAATTAAATCAGCCCAT
AAAATTGCAAAGAGGTACTCTTCCATCCCTCAGATGTGGTCTAGGTGTCT
ACTGCGCCACTGTTATGGACTGTGGTTTATTTGTCTCCCAGCTTATGTGA
AAGTCTGTCATTCAAAAGTCAGGGCTCTGAAAACAGCATATGATGTGCTT
AAAAAAATGCAGTCAAAGAAGATGGATCCACCTGATGAGGTATGCTACCG
CATTCTTATGCAACTCTGTGGACAATATGATCAGCCAGTGCTTGCAGTTC
GAGTGCTTTTTGAAATGCAGAAAGCTGGTATTGACCCCAATGCCATTACT
TATGGTTATTATAATAAGGCTGTTTTGGAAAGTACCTGGCCTTCAAGAAG
TCGTAGTGGCTATTTTCTTTGGACAAAAGTAAGAAATGTTGTTTTAGGAG
TAACACAGTTCAAAAGAGCTTTAAAGAAGCATGCACACTTATCACAAACA
ACTCTCTCAGGTGGCCAGTCTGATCTTGGATATAATTCATTATCTAAAGA
TGAAGTTAGAAGAGGGGATACATCTACTGAAGACATTCAAGAAGAAAAAA
AGAAAAAATAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTI
DAAAMIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPIT
DVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRD
KPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRR
ASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCY
KKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPS
NSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENPTEKQRLLLGLTS
ADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIE
KHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTL
LQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPC
PYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQ
IGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAIND
YDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAA
SLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFD
DCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYS
YNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSA
HKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDV
LKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAI
TYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQ
TTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKKKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36708
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with Q05C90:; L284P. Derives from early IRLB clones.