cDNA Clone Aurora B

• Synonyms: AURKB
• Plasmid: pGEX6P-3-Aurora B
• Parent Plasmid: pGEX6P3
• Expressed: GST-Aurora B
• Genbank: NM_004217.2 GI:83776599*
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGGCCCAGAAGGAGAACTCCTACCCCTGGCCCTACGGCCGACA
GACGGCTCCATCTGGCCTGAGCACCCTGCCCCAGCGAGTCCTCCGGAAAG
AGCCTGTCACCCCATCTGCACTTGTCCTCATGAGCCGCTCCAATGTCCAG
CCCACAGCTGCCCCTGGCCAGAAGGTGATGGAGAATAGCAGTGGGACACC
CGACATCTTAACGCGGCACTTCACAATTGATGACTTTGAGATTGGGCGTC
CTCTGGGCAAAGGCAAGTTTGGAAACGTGTACTTGGCTCGGGAGAAGAAA
AGCCATTTCATCGTGGCGCTCAAGGTCCTCTTCAAGTCCCAGATAGAGAA
GGAGGGCGTGGAGCATCAGCTGCGCAGAGAGATCGAAATCCAGGCCCACC
TGCACCATCCCAACATCCTGCGTCTCTACAACTATTTTTATGACCGGAGG
AGGATCTACTTGATTCTAGAGTATGCCCCCCGCGGGGAGCTCTACAAGGA
GCTGCAGAAGAGCTGCACATTTGACGAGCAGCGAACAGCCACGATCATGG
AGGAGTTGGCAGATGCTCTAATGTACTGCCATGGGAAGAAGGTGATTCAC
AGAGACATAAAGCCAGAAAATCTGCTCTTAGGGCTCAAGGGAGAGCTGAA
GATTGCTGACTTCGGCTGGTCTGTGCATGCGCCCTCCCTGAGGAGGAAGA
CAATGTGTGGCACCCTGGACTACCTGCCCCCAGAGATGATTGAGGGGCGC
ATGCACAATGAGAAGGTGGATCTGTGGTGCATTGGAGTGCTTTGCTATGA
GCTGCTGGTGGGGAACCCACCCTTTGAGAGTGCATCACACAACGAGACCT
ATCGCCGCATCGTCAAGGTGGACCTAAAGTTCCCCGCTTCTGTGCCCACG
GGAGCCCAGGACCTCATCTCCAAACTGCTCAGGCATAACCCCTCGGAACG
GCTGCCCCTGGCCCAGGTCTCAGCCCACCCTTGGGTCCGGGCCAACTCTC
GGAGGGTGCTGCCTCCCTCTGCCCTTCAATCTGTCGCCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAQKENSYPWPYGRQTAPS
GLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILT
RHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVE
HQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKS
CTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADF
GWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVG
NPPFESASHNETYRRIVKVDLKFPASVPTGAQDLISKLLRHNPSERLPLA
QVSAHPWVRANSRRVLPPSALQSVA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36687
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differnces compared with NM_004217.2 CDS:; c885t (silent); t893c (M298T); Both derive from MP clone DU1773 used as template, and match AAV38341.1; Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 66102 Daltons