cDNA Clone AS160 (mouse)

• Synonyms: TBC1D4
• Plasmid: pGEX6P-3 AS160 (mouse) M1-K609
• Parent Plasmid: pGEX6P3
• Expressed: GST AS160 (mouse) M1-K609
• Genbank: NM_001081278.2 GI:163644269
• Tag: GST
• Species: Mouse

Sequence of Insert

AGATCTATGGAGTCGCCTAGCTGCATTCAGGATGAGCCTTTCCCGCACCC
CCTGGAGCCTGAGCCGAGTGCCCCAGCACAGCCAGGTGCCACCAAGCCTG
GCGACAAGCGCTTCCGGCTATGGTACGTGGGAGGGTCTTGCTTGGATCGC
AGGACCACGCTACCCATGCTCCCTTGGCTCATGGCCGAGATCCGTCGGCG
CAGCCAGAAGCCCGATGCGGGCGGCTGCGGGGCGCCCGCAGCCCGGGAGG
TGATCCTGGTGCTCAGCGCCCCCTTTCTGCGTTGTGTCCCTGCGCCGGGT
GCCGGGGTCGGCGGGGGCGCAGGCTCCGGGGCTGTGCAGCCCAACACCGG
GGTGTTCATCTTCGAGCACAAGGCTCAGCACATCTCGCGCTTCATCCACA
ACAGTCACGACCTCACCTACTTTGCCTATTTGATCAAGGCGCAGCCCGAC
GACCCCGAGTCACAGATGGCCTGCCACGTTTTCCGCGCCACGGATCCTAA
CCAGGTTCCAGATGTGATCAGTAGCATCAGGCAGTTATCCAAGGCTGCCA
TGAAGGAGGACTCCAAACCCAGCAAAGATAACGAGGATGCCTTCTACAAC
TCTCAGAAATTCGAAGTGCTTTACTGTGGGCGGGTGATCGTGACCCATAA
GAAGGCGCCCTCCAGCCTCATTGATGACTGCAAGGACAAGTTCAGCCTGC
ATGAGCAGCAGCGACTGAAGCTGCAAGGCGAGAGAGGTGGTGACCCTGGC
GACGAGATGGGTGTCCTTGAGGTCGAGTCTCCTGTGTCTCCAGATGACAG
CCTGCCCGAGAAGGCAGATGGTACAGTCAACAGCCCCCGTGCCCTGCCCT
CTCTGGCTAGCCTTCCTGCCTTGGCCAGCCAACCCGCACTAGCCAGCTCT
CGGGTTTGCTTCCCAGAGAGGATTTTGGAAGACTGTGGCTTTGACGAGCA
GCAAGAGTTCCGATCTCGGTGCAGCAGCGTCACAGGCGTCATGCAAAAGA
AGGTTCATGAGAACAACCAGAAAACACAGCCAAGAAGGAGGCACGCGAGC
GCGCCCAGTCACGTGCAGCCCTCAGACTCGGAGAAGAATCGGACCATGCT
CTTCCAGGTCGGCCGCTTTGAGATTAACCTCATCAGTCCCGACACTAAAT
CAGTCGTGCTGGAGAAGAATTTCAAAGATATCTCCTCTTGTTCTCAGGGA
ATAAAGCATGTTGATCACTTTGGCTTTATCTGTCGGGAGTCTCCGGAGCC
GGGGCTGAGCCAGTACATCTGTTATGTGTTCCAGTGCGCCAACGAATCTC
TGGTGGATGAGGTCATGCTGACTCTGAAGCAAGCTTTCAGCACGGCTGCT
GCGCTGCAGAGTGCCAAGACGCAGATTAAACTGTGCGAGACCTGCCCCAT
GCACTCTTTGCATAAGCTCTGTGAGAGAATCGAAGGTCTCTACCCACCTC
GAGCCAAGCTGGTAATCCAGAGGCACCTCTCTTCGCTGACGGATAACGAG
CAAGCTGACATCTTTGAACGGGTTCAGAAGATGAAGCCCATCAGCGACCA
GGAGGAGAACGAACTTGTGATTCTGCACCTGAGGCAGCTCTGTGAAGCCA
AGCAGAGGACCCACGTGCACATCGGGGAAGGGCCGGCGATTATTTCAAAT
AGTACAATCCCAGAAAATGTGACAAGCGGTGGGAGGTTCAAGCTTGATGT
TCTGAAAAACAAAGCGAAGAGATCCTTAACTTCCTCCCTGGAAAATATCT
TCTCCAGGGGAGCTAACAGGATGAGAGGTCGGCTTGGAAGTATGGACAGC
TTTGAACGCGCCAACAGTCTTGCCTCAGAGAAGTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESPSCIQDEPFPHPLEPE
PSAPAQPGATKPGDKRFRLWYVGGSCLDRRTTLPMLPWLMAEIRRRSQKP
DAGGCGAPAAREVILVLSAPFLRCVPAPGAGVGGGAGSGAVQPNTGVFIF
EHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPNQVPD
VISSIRQLSKAAMKEDSKPSKDNEDAFYNSQKFEVLYCGRVIVTHKKAPS
SLIDDCKDKFSLHEQQRLKLQGERGGDPGDEMGVLEVESPVSPDDSLPEK
ADGTVNSPRALPSLASLPALASQPALASSRVCFPERILEDCGFDEQQEFR
SRCSSVTGVMQKKVHENNQKTQPRRRHASAPSHVQPSDSEKNRTMLFQVG
RFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQ
YICYVFQCANESLVDEVMLTLKQAFSTAAALQSAKTQIKLCETCPMHSLH
KLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPISDQEENE
LVILHLRQLCEAKQRTHVHIGEGPAIISNSTIPENVTSGGRFKLDVLKNK
AKRSLTSSLENIFSRGANRMRGRLGSMDSFERANSLASEK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36667
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 94225 Daltons