cDNA Clone TCF3 (mouse)

• Synonyms: A1, AA408400, ALF2, AW209082, bHLHb21, E12, E47
• Plasmid: pGEX6P-3 E47 (TCF3) (mouse) -6His
• Parent Plasmid: pGEX6P3
• Expressed: GST-E47 (TCF3) (mouse)-6His
• Genbank: NM_001164150.1 GI:255918159
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGATGAACCAGTCTCAGAGAATGGCACCCGTGGGCTCTGACAA
GGAACTGAGTGACCTCCTGGACTTCAGCATGATGTTCCCGCTACCTGTGG
CCAATGGGAAGAGCCGGCCCGCCTCCCTCGGGGGAACCCAGTTTGCAGGC
TCAGGACTGGAGGACCGACCCAGCTCAGGCTCCTGGGGCAGCAGTGACCA
GAACAGTTCTTCCTTTGACCCTAGCCGGACATACAGCGAAGGTGCCCACT
TCAGTGACTCCCACAGCAGCCTGCCGCCTTCCACGTTCCTAGGAGCTGGG
CTTGGAGGCAAGGGCAGTGAGCGGAATGCCTATGCCACCTTTGGGAGAGA
CACCAGTGTTGGCACCTTGAGTCAGGCTGGCTTCCTGCCAGGTGAGCTGA
GCCTCAGCAGTCCCGGGCCACTGTCCCCATCGGGCATCAAGAGCAGCTCC
CAGTATTACCCCTCATTCCCCAGCAACCCTCGTCGGAGAGCTGCAGATGG
TGGCCTGGCAGATACTCAGCCGAAGAAGGTCCGGAAGGTTCCGCCTGGTC
TCCCTTCCTCGGTGTATCCGCCCAGCTCAGGTGACAGCTACAGCAGGGAT
GCTGCAGCCTACCCCTCCGCCAAGACCCCCAGCAGCGCTTACCCCTCCCC
CTTCTACGTGGCAGATGGCAGCCTGCACCCATCAGCTGAGCTCTGGAGTA
CGCCTAGCCAGGTGGGCTTTGGGCCCATGCTAGGTGACGGCTCTTCCCCT
CTGCCCCTTGCACCGGGCAGCAGCTCCGTGGGCAGTGGTACCTTTGGGGG
CCTCCAGCAGCAGGATCGCATGGGCTACCAGCTGCATGGATCTGAGGTTA
ATGGCTCGCTCCCAGCTGTATCCAGCTTTTCGGCTGCCCCTGGCACTTAC
AGTGGGACTTCCGGCCACACGCCCCCTGTGAGTGGGGCCGCAGCTGAAAG
CCTCCTAGGCACCCGAGGGACTACAGCCAGCAGCTCAGGGGATGCCCTTG
GGAAGGCACTGGCCTCGATCTACTCCCCGGATCACTCCAGCAATAATTTC
TCACCTAGCCCCTCAACGCCTGTGGGTTCACCCCAGGGCCTGCCAGGGAC
ATCACAGTGGCCCCGGGCAGGAGCGCCCAGTGCCTTATCCCCCAACTACG
ATGCAGGTCTCCATGGCCTGCAGAGCAAGATGGAGGACCGCTTGGACGAG
GCCATCCATGTCCTGCGAAGCCACGCTGTTGGCACCGCTAGCGATCTCCA
TGGGCTTTTGCCTGGCCATGGCGCACTGACCACGAGCTTCACCGGCCCCA
TGTCACTGGGCGGGCGGCATGCCGGCCTGGTCGGGGGAAGCCATCCTGAG
GAGGGCCTCACAAGTGGGGCCAGTCTTTTGCATAACCATGCCAGCCTCCC
CAGCCAGCCCAGTTCCCTCCCTGACCTCTCACAGAGACCTCCCGACTCCT
ATAGTGGACTCGGGAGGGCAGGCACAACAGCGGGTGCCAGCGAGATCAAG
CGGGAGGAGAAAGAGGATGAGGAAATCGCATCAGTAGCCGACGCCGAAGA
GGACAAGAAGGACCTGAAGGTCCCACGCACGCGCACCAGCAGTACAGATG
AGGTGCTGTCCCTGGAGGAGAAGGACCTGAGGGACCGGGAGAGGCGTATG
GCCAATAACGCTCGGGAGCGGGTGCGCGTGCGGGACATTAACGAGGCCTT
CCGGGAGCTGGGCCGCATGTGCCAGCTGCACCTCAAGTCGGATAAGGCGC
AGACCAAGCTGCTCATCCTGCAGCAGGCGGTGCAGGTCATCCTGGGCCTG
GAGCAGCAGGTGCGAGAACGCAACCTGAACCCCAAAGCAGCCTGCTTGAA
GCGGAGGGAGGAGGAGAAGGTGTCTGGCGTGGTCGGGGACCCACAGCTGG
CCCTGTCAGCCGCCCACCCGGGCCTGGGTGAGGCCCACAACCCAGCCGGG
CACCTGCACCATCACCATCACCATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMMNQSQRMAPVGSDKELSD
LLDFSMMFPLPVANGKSRPASLGGTQFAGSGLEDRPSSGSWGSSDQNSSS
FDPSRTYSEGAHFSDSHSSLPPSTFLGAGLGGKGSERNAYATFGRDTSVG
TLSQAGFLPGELSLSSPGPLSPSGIKSSSQYYPSFPSNPRRRAADGGLAD
TQPKKVRKVPPGLPSSVYPPSSGDSYSRDAAAYPSAKTPSSAYPSPFYVA
DGSLHPSAELWSTPSQVGFGPMLGDGSSPLPLAPGSSSVGSGTFGGLQQQ
DRMGYQLHGSEVNGSLPAVSSFSAAPGTYSGTSGHTPPVSGAAAESLLGT
RGTTASSSGDALGKALASIYSPDHSSNNFSPSPSTPVGSPQGLPGTSQWP
RAGAPSALSPNYDAGLHGLQSKMEDRLDEAIHVLRSHAVGTASDLHGLLP
GHGALTTSFTGPMSLGGRHAGLVGGSHPEEGLTSGASLLHNHASLPSQPS
SLPDLSQRPPDSYSGLGRAGTTAGASEIKREEKEDEEIASVADAEEDKKD
LKVPRTRTSSTDEVLSLEEKDLRDRERRMANNARERVRVRDINEAFRELG
RMCQLHLKSDKAQTKLLILQQAVQVILGLEQQVRERNLNPKAACLKRREE
EKVSGVVGDPQLALSAAHPGLGEAHNPAGHLHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36609
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express E47 with N-terminal GST and C-terminal 6His ; ; Estimated MW of expressed protein : 95259 Daltons