cDNA Clone ATP1A1

• Plasmid: pGEX6P-3-ATP1A1 T339-L772-FLAG
• Parent Plasmid: pGEX6P3
• Expressed: GST-ATP1A1 339-772-FLAG
• Genbank: NM_000701.6 GI:48762680
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCTGCCACCACTGTCACGGTCTGTCTGACACTTACTGCCAAACGCAT
GGCAAGGAAAAACTGCTTAGTGAAGAACTTAGAAGCTGTGGAGACCTTGG
GGTCCACGTCCACCATCTGCTCTGATAAAACTGGAACTCTGACTCAGAAC
CGGATGACAGTGGCCCACATGTGGTTTGACAATCAAATCCATGAAGCTGA
TACGACAGAGAATCAGAGTGGTGTCTCTTTTGACAAGACTTCAGCTACCT
GGCTTGCTCTGTCCAGAATTGCAGGTCTTTGTAACAGGGCAGTGTTTCAG
GCTAACCAGGAAAACCTACCTATTCTTAAGCGGGCAGTTGCAGGAGATGC
CTCTGAGTCAGCACTCTTAAAGTGCATAGAGCTGTGCTGTGGTTCCGTGA
AGGAGATGAGAGAAAGATACGCCAAAATCGTCGAGATACCCTTCAACTCC
ACCAACAAGTACCAGTTGTCTATTCATAAGAACCCCAACACATCGGAGCC
CCAACACCTGTTGGTGATGAAGGGCGCCCCAGAAAGGATCCTAGACCGTT
GCAGCTCTATCCTCCTCCACGGCAAGGAGCAGCCCCTGGATGAGGAGCTG
AAAGACGCCTTTCAGAACGCCTATTTGGAGCTGGGGGGCCTCGGAGAACG
AGTCCTAGGTTTCTGCCACCTCTTTCTGCCAGATGAACAGTTTCCTGAAG
GGTTCCAGTTTGACACTGACGATGTGAATTTCCCTATCGATAATCTGTGC
TTTGTTGGGCTCATCTCCATGATTGACCCTCCACGGGCGGCCGTTCCTGA
TGCCGTGGGCAAATGTCGAAGTGCTGGAATTAAGGTCATCATGGTCACAG
GAGACCATCCAATCACAGCTAAAGCTATTGCCAAAGGTGTGGGCATCATC
TCAGAAGGCAATGAGACCGTGGAAGACATTGCTGCCCGCCTCAACATCCC
AGTCAGCCAGGTGAACCCCAGGGATGCCAAGGCCTGCGTAGTACACGGCA
GTGATCTAAAGGACATGACCTCCGAGCAGCTGGATGACATTTTGAAGTAC
CACACTGAGATAGTGTTTGCCAGGACCTCCCCTCAGCAGAAGCTCATCAT
TGTGGAAGGCTGCCAAAGACAGGGTGCTATCGTGGCTGTGACTGGTGACG
GTGTGAATGACTCTCCAGCTTTGAAGAAAGCAGACATTGGGGTTGCTATG
GGGATTGCTGGCTCAGATGTGTCCAAGCAAGCTGCTGACATGATTCTTCT
GGATGACAACTTTGCCTCAATTGTGACTGGAGTAGAGGAAGGTCGTCTGA
TCTTTGATAACTTGGACTACAAGGACGACGATGACAAGTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATTVTVCLTLTAKRMARKN
CLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEN
QSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESA
LLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLL
VMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGF
CHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGK
CRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQV
NPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGC
QRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNF
ASIVTGVEEGRLIFDNLDYKDDDDK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36607
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 75116 Daltons