cDNA Clone TEC

• Synonyms: MGC126760, MGC126762, PSCTK4
• Plasmid: pEBG6P-1 TEC
• Parent Plasmid: pEBG6P
• Expressed: GST-TEC
• Genbank: NM_003215.2 GI:130977758
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTC
ACAGCAGAAAAAGAAGACATCGCCCTTAAACTACAAAGAGAGACTTTTTG
TACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAAGAAA
TACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGT
GAAGAATGATGATGGTGTCATTCCCTGTCAAAATAAGTATCCATTTCAGG
TTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGTCCACAAAGC
AGGGACCTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAA
TATTATGATTAAATATCATCCTAAATTCTGGACAGATGGAAGTTATCAGT
GTTGTAGACAAACTGAAAAATTAGCACCCGGATGTGAAAAATACAATCTT
TTTGAGAGCAGTATAAGAAAAGCACTACCTCCAGCACCAGAAACAAAGAA
GCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGTGAAG
AAATCGTTGTAGCCATGTATGATTTCCAAGCAGCAGAAGGACATGATCTC
AGATTAGAGAGAGGCCAAGAGTATCTCATTTTAGAAAAGAATGATGTTCA
TTGGTGGAGAGCAAGAGATAAATATGGGAATGAAGGATATATCCCAAGTA
ATTACGTAACGGGAAAGAAATCAAACAACTTAGATCAATATGAATGGTAT
TGCAGAAATATGAATAGAAGCAAGGCAGAGCAACTCCTCCGCAGTGAAGA
TAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTGTACA
CAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGG
CATTATCATATAAAGGAAACAACAACATCTCCAAAGAAGTATTACCTAGC
TGAAAAACATGCTTTTGGCTCCATTCCTGAGATTATTGAATATCATAAGC
ACAATGCAGCAGGACTTGTCACCAGGCTTCGGTACCCAGTTAGTGTGAAA
GGGAAGAATGCACCCACCACTGCAGGATTCAGCTATGAGAAATGGGAGAT
TAACCCTTCAGAACTGACCTTTATGAGGGAATTGGGAAGTGGACTGTTTG
GAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAATCAAA
GCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAA
AGTGATGATGAAACTGACACACCCGAAGTTAGTGCAGCTTTATGGTGTGT
GCACCCAGCAGAAACCAATATACATTGTTACTGAGTTCATGGAAAGGGGC
TGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCATTTCAGTAGAGACGT
ACTGCTGAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTGGAGA
GAAACAGCTTCATCCACAGAGATCTGGCTGCCAGAAATTGTCTAGTAAGT
GAGGCGGGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATGTTCT
GGATGATCAGTACACAAGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGT
GTCCACCTGAAGTGTTTAATTACAGCCGCTTCAGCAGCAAATCAGATGTC
TGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACGGAAGGCAGAATGCC
TTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGAGGCC
ACCGACTCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATG
CTGAGATGTTGGCAGGAGAAACCAGAGGGAAGGCCTTCTTTCGAAGATCT
GCTGCGCACAATAGATGAACTAGTTGAATGTGAAGAAACTTTTGGAAGAT
AAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMNFNTILEEILIK
RSQQKKKTSPLNYKERLFVLTKSMLTYYEGRAEKKYRKGFIDVSKIKCVE
IVKNDDGVIPCQNKYPFQVVHDANTLYIFAPSPQSRDLWVKKLKEEIKNN
NNIMIKYHPKFWTDGSYQCCRQTEKLAPGCEKYNLFESSIRKALPPAPET
KKRRPPPPIPLEEEDNSEEIVVAMYDFQAAEGHDLRLERGQEYLILEKND
VHWWRARDKYGNEGYIPSNYVTGKKSNNLDQYEWYCRNMNRSKAEQLLRS
EDKEGGFMVRDSSQPGLYTVSLYTKFGGEGSSGFRHYHIKETTTSPKKYY
LAEKHAFGSIPEIIEYHKHNAAGLVTRLRYPVSVKGKNAPTTAGFSYEKW
EINPSELTFMRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEE
AKVMMKLTHPKLVQLYGVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSR
DVLLSMCQDVCEGMEYLERNSFIHRDLAARNCLVSEAGVVKVSDFGMARY
VLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEVFTEGR
MPFEKYTNYEVVTMVTRGHRLYQPKLASNYVYEVMLRCWQEKPEGRPSFE
DLLRTIDELVECEETFGR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36462
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express TEC with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 100996 Daltons