cDNA Clone EPHB6

• Plasmid: pGEX6P-3-EPHB6 E660-end (lacks transmembrane domain)
• Parent Plasmid: pGEX6P3
• Expressed: GST-EPHB6 E660-end
• Genbank: NM_004445.3 GI:194097331
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGAACTTGCCCGGGAAGTCGATCCTGCTTATATCAAGATTGAGGA
GGTCATTGGGACAGGCTCTTTTGGAGAAGTGCGCCAGGGCCGCCTGCAGC
CACGGGGACGGAGGGAGCAGACTGTGGCCATCCAGGCCCTGTGGGCCGGG
GGCGCCGAAAGCCTGCAGATGACCTTCCTGGGCCGGGCCGCAGTGCTGGG
TCAGTTCCAGCACCCCAACATCCTGCGGCTGGAGGGCGTGGTCACCAAGA
GCCGACCCCTCATGGTGCTGACGGAGTTCATGGAGCTTGGCCCCCTGGAC
AGCTTCCTCAGGCAGCGGGAGGGCCAGTTCAGCAGCCTGCAGCTGGTGGC
CATGCAGCGGGGAGTGGCTGCTGCCATGCAGTACCTGTCCAGCTTTGCCT
TCGTCCATCGCTCGCTGTCTGCCCACAGCGTGCTGGTGAATAGCCACTTG
GTGTGCAAGGTGGCCCGTCTTGGCCACAGTCCTCAGGGCCCAAGTTGTTT
GCTTCGCTGGGCAGCCCCAGAGGTCATTGCACATGGAAAGCATACAACAT
CCAGTGATGTCTGGAGCTTTGGGATACTCATGTGGGAAGTGATGAGTTAT
GGAGAACGGCCTTACTGGGACATGAGTGAGCAGGAGGTACTAAATGCAAT
AGAGCAGGAGTTCCGGCTGCCCCCGCCTCCAGGCTGTCCTCCTGGATTAC
ATCTACTTATGTTGGACACTTGGCAGAAGGACCGTGCCCGGCGGCCTCAT
TTTGACCAGCTGGTGGCTGCATTTGACAAGATGATCCGCAAGCCAGATAC
CCTGCAGGCTGGCGGGGACCCAGGGGAAAGGCCTTCCCAGGCCCTTCTGA
CCCCTGTGGCCCTGGACTTTCCTTGTCTGGACTCACCCCAGGCCTGGCTT
TCAGCCATTGGACTGGAGTGCTACCAGGACAACTTCTCCAAGTTTGGCCT
CTGTACCTTCAGTGATGTGGCTCAGCTCAGCCTAGAAGACCTGCCTGCCC
TGGGCATCACCCTGGCTGGCCACCAGAAGAAGCTGCTGCACCACATCCAG
CTCCTTCAGCAACACCTGAGGCAGCAGGGCTCAGTGGAGGTCTGAGCGGC
CGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSELAREVDPAYIKIEEVIGT
GSFGEVRQGRLQPRGRREQTVAIQALWAGGAESLQMTFLGRAAVLGQFQH
PNILRLEGVVTKSRPLMVLTEFMELGPLDSFLRQREGQFSSLQLVAMQRG
VAAAMQYLSSFAFVHRSLSAHSVLVNSHLVCKVARLGHSPQGPSCLLRWA
APEVIAHGKHTTSSDVWSFGILMWEVMSYGERPYWDMSEQEVLNAIEQEF
RLPPPPGCPPGLHLLMLDTWQKDRARRPHFDQLVAAFDKMIRKPDTLQAG
GDPGERPSQALLTPVALDFPCLDSPQAWLSAIGLECYQDNFSKFGLCTFS
DVAQLSLEDLPALGITLAGHQKKLLHHIQLLQQHLRQQGSVEV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36434
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 67122 Daltons