cDNA Clone EPHB6

• Plasmid: pGEX6P-3-EPHB6 (includes transmembrane domain)
• Parent Plasmid: pGEX6P3
• Expressed: GST-EPHB6
• Genbank: NM_004445.3 GI:194097331
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCTACTGAAGGGGCTGCCCAGTTAGGGAACAGAGTGGCGGG
CATGGTGTGTAGCCTATGGGTGCTGCTCCTGGTGTCTTCAGTTCTGGCTC
TGGAAGAGGTATTGCTGGACACCACCGGAGAGACATCTGAGATTGGCTGG
CTCACCTACCCACCAGGGGGGTGGGACGAGGTGAGTGTTCTGGACGACCA
GCGACGCCTGACTCGGACCTTTGAGGCATGTCATGTGGCAGGGGCCCCTC
CAGGCACCGGGCAGGACAATTGGTTGCAGACACACTTTGTGGAGCGGCGC
GGGGCCCAGAGGGCGCACATTCGACTCCACTTCTCTGTGCGGGCATGCTC
CAGCCTGGGTGTGAGCGGCGGCACCTGCCGGGAGACCTTCACCCTTTACT
ACCGTCAGGCTGAGGAGCCCGACAGCCCTGACAGCGTTTCCTCCTGGCAC
CTCAAACGCTGGACCAAGGTGGACACAATTGCAGCAGACGAGAGCTTTCC
CTCCTCCTCCTCCTCCTCCTCCTCTTCTTCCTCTGCAGCGTGGGCTGTGG
GACCCCACGGGGCTGGGCAGCGGGCTGGACTGCAACTGAACGTCAAAGAG
CGGAGCTTTGGGCCTCTCACCCAACGCGGCTTCTACGTGGCCTTCCAGGA
CACGGGGGCCTGCCTGGCCCTGGTCGCTGTCAGGCTCTTCTCCTACACCT
GCCCTGCCGTGCTCCGATCCTTTGCTTCCTTTCCAGAGACGCAGGCCAGT
GGGGCTGGGGGGGCCTCCCTGGTGGCAGCTGTGGGCACCTGTGTGGCTCA
TGCAGAGCCAGAGGAGGATGGAGTAGGGGGCCAGGCAGGAGGCAGCCCCC
CCAGGCTGCACTGCAACGGGGAGGGCAAGTGGATGGTAGCTGTCGGGGGC
TGCCGCTGCCAGCCTGGATACCAACCAGCACGAGGAGACAAGGCCTGCCA
AGCCTGCCCACGGGGGCTCTATAAGTCTTCTGCTGGGAATGCTCCCTGCT
CACCATGCCCTGCCCGCAGTCACGCTCCCAACCCAGCAGCCCCCGTTTGC
CCCTGCCTGGAGGGCTTCTACCGGGCCAGTTCCGACCCACCAGAGGCCCC
CTGCACTGGTCCTCCATCGGCTCCCCAGGAGCTTTGGTTTGAGGTGCAAG
GCTCAGCACTCATGCTACACTGGCGCCTGCCTCGGGAGCTGGGGGGTCGA
GGGGACCTGCTCTTCAATGTCGTGTGCAAGGAGTGTGAAGGCCGCCAGGA
ACCTGCCAGCGGTGGTGGGGGCACTTGTCACCGCTGCAGGGATGAGGTCC
ACTTCGACCCTCGCCAGAGAGGCCTGACTGAGAGCCGAGTGTTAGTGGGG
GGACTCCGGGCACACGTACCCTACATCTTAGAGGTGCAGGCTGTTAATGG
GGTGTCTGAGCTCAGCCCTGACCCTCCTCAGGCTGCAGCCATCAATGTCA
GCACCAGCCATGAAGTGCCCTCTGCTGTCCCTGTGGTGCACCAGGTGAGC
CGGGCATCCAACAGCATCACGGTGTCCTGGCCGCAGCCCGACCAGACCAA
TGGGAACATCCTGGACTATCAGCTCCGCTACTATGACCAGGCAGAAGACG
AATCCCACTCCTTCACCCTGACCAGCGAGACCAACACTGCCACCGTGACA
CAGCTGAGCCCTGGCCACATCTATGGTTTCCAGGTGCGGGCCCGGACTGC
TGCCGGCCACGGCCCCTACGGGGGCAAAGTCTATTTCCAGACACTTCCTC
AAGGGGAGCTGTCTTCCCAGCTTCCGGAAAGACTCTCCTTGGTGATCGGC
TCCATCCTGGGGGCTTTGGCCTTCCTCCTGCTGGCAGCCATCACCGTGCT
GGCGGTCGTCTTCCAGCGGAAGCGGCGTGGGACTGGCTACACGGAGCAGC
TGCAGCAATACAGCAGCCCAGGACTCGGGGTGAAGTATTACATCGACCCC
TCCACCTACGAGGACCCCTGTCAGGCCATCCGAGAACTTGCCCGGGAAGT
CGATCCTGCTTATATCAAGATTGAGGAGGTCATTGGGACAGGCTCTTTTG
GAGAAGTGCGCCAGGGCCGCCTGCAGCCACGGGGACGGAGGGAGCAGACT
GTGGCCATCCAGGCCCTGTGGGCCGGGGGCGCCGAAAGCCTGCAGATGAC
CTTCCTGGGCCGGGCCGCAGTGCTGGGTCAGTTCCAGCACCCCAACATCC
TGCGGCTGGAGGGCGTGGTCACCAAGAGCCGACCCCTCATGGTGCTGACG
GAGTTCATGGAGCTTGGCCCCCTGGACAGCTTCCTCAGGCAGCGGGAGGG
CCAGTTCAGCAGCCTGCAGCTGGTGGCCATGCAGCGGGGAGTGGCTGCTG
CCATGCAGTACCTGTCCAGCTTTGCCTTCGTCCATCGCTCGCTGTCTGCC
CACAGCGTGCTGGTGAATAGCCACTTGGTGTGCAAGGTGGCCCGTCTTGG
CCACAGTCCTCAGGGCCCAAGTTGTTTGCTTCGCTGGGCAGCCCCAGAGG
TCATTGCACATGGAAAGCATACAACATCCAGTGATGTCTGGAGCTTTGGG
ATACTCATGTGGGAAGTGATGAGTTATGGAGAACGGCCTTACTGGGACAT
GAGTGAGCAGGAGGTACTAAATGCAATAGAGCAGGAGTTCCGGCTGCCCC
CGCCTCCAGGCTGTCCTCCTGGATTACATCTACTTATGTTGGACACTTGG
CAGAAGGACCGTGCCCGGCGGCCTCATTTTGACCAGCTGGTGGCTGCATT
TGACAAGATGATCCGCAAGCCAGATACCCTGCAGGCTGGCGGGGACCCAG
GGGAAAGGCCTTCCCAGGCCCTTCTGACCCCTGTGGCCCTGGACTTTCCT
TGTCTGGACTCACCCCAGGCCTGGCTTTCAGCCATTGGACTGGAGTGCTA
CCAGGACAACTTCTCCAAGTTTGGCCTCTGTACCTTCAGTGATGTGGCTC
AGCTCAGCCTAGAAGACCTGCCTGCCCTGGGCATCACCCTGGCTGGCCAC
CAGAAGAAGCTGCTGCACCACATCCAGCTCCTTCAGCAACACCTGAGGCA
GCAGGGCTCAGTGGAGGTCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATEGAAQLGNRVAGMVCS
LWVLLLVSSVLALEEVLLDTTGETSEIGWLTYPPGGWDEVSVLDDQRRLT
RTFEACHVAGAPPGTGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGV
SGGTCRETFTLYYRQAEEPDSPDSVSSWHLKRWTKVDTIAADESFPSSSS
SSSSSSSAAWAVGPHGAGQRAGLQLNVKERSFGPLTQRGFYVAFQDTGAC
LALVAVRLFSYTCPAVLRSFASFPETQASGAGGASLVAAVGTCVAHAEPE
EDGVGGQAGGSPPRLHCNGEGKWMVAVGGCRCQPGYQPARGDKACQACPR
GLYKSSAGNAPCSPCPARSHAPNPAAPVCPCLEGFYRASSDPPEAPCTGP
PSAPQELWFEVQGSALMLHWRLPRELGGRGDLLFNVVCKECEGRQEPASG
GGGTCHRCRDEVHFDPRQRGLTESRVLVGGLRAHVPYILEVQAVNGVSEL
SPDPPQAAAINVSTSHEVPSAVPVVHQVSRASNSITVSWPQPDQTNGNIL
DYQLRYYDQAEDESHSFTLTSETNTATVTQLSPGHIYGFQVRARTAAGHG
PYGGKVYFQTLPQGELSSQLPERLSLVIGSILGALAFLLLAAITVLAVVF
QRKRRGTGYTEQLQQYSSPGLGVKYYIDPSTYEDPCQAIRELAREVDPAY
IKIEEVIGTGSFGEVRQGRLQPRGRREQTVAIQALWAGGAESLQMTFLGR
AAVLGQFQHPNILRLEGVVTKSRPLMVLTEFMELGPLDSFLRQREGQFSS
LQLVAMQRGVAAAMQYLSSFAFVHRSLSAHSVLVNSHLVCKVARLGHSPQ
GPSCLLRWAAPEVIAHGKHTTSSDVWSFGILMWEVMSYGERPYWDMSEQE
VLNAIEQEFRLPPPPGCPPGLHLLMLDTWQKDRARRPHFDQLVAAFDKMI
RKPDTLQAGGDPGERPSQALLTPVALDFPCLDSPQAWLSAIGLECYQDNF
SKFGLCTFSDVAQLSLEDLPALGITLAGHQKKLLHHIQLLQQHLRQQGSV
EV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36419
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 137518 Daltons