cDNA Clone USP27X

• Synonyms: USP22L, USP27
• Plasmid: pFB.HTb USP27X
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-USP27X
• Genbank: NM_001145073.1 GI:223005905
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTGTAAGGACTATGTATATGACAAAGACATTGAGCAAATTGC
CAAAGAAGAGCAAGGAGAAGCTTTGAAATTACAAGCCTCCACCTCAACAG
AGGTTTCTCACCAGCAGTGTTCAGTGCCAGGCCTTGGTGAGAAATTCCCA
ACCTGGGAAACAACCAAACCAGAATTAGAACTGCTGGGGCACAACCCGAG
GAGAAGAAGAATCACCTCCAGCTTTACGATCGGTTTAAGAGGACTCATCA
ATCTTGGCAACACGTGCTTTATGAACTGCATTGTCCAGGCCCTCACCCAC
ACGCCGATACTGAGAGATTTCTTTCTCTCTGACAGGCACCGATGTGAGAT
GCCGAGTCCCGAGTTGTGTCTGGTCTGTGAGATGTCGTCGCTGTTTCGGG
AGTTGTATTCTGGAAACCCGTCTCCTCATGTGCCCTATAAGTTACTGCAC
CTGGTGTGGATACATGCCCGCCATTTAGCAGGGTACAGGCAACAGGATGC
CCACGAGTTCCTCATTGCAGCGTTAGATGTCCTGCACAGGCACTGCAAAG
GTGATGATGTCGGGAAGGCGGCCAACAATCCCAACCACTGTAACTGCATC
ATAGACCAAATCTTCACAGGTGGCCTGCAGTCTGATGTCACCTGTCAAGC
CTGCCATGGCGTCTCCACCACGATAGACCCATGCTGGGACATTAGTTTGG
ACTTGCCTGGCTCTTGCACCTCCTTCTGGCCCATGAGCCCAGGGAGGGAG
AGCAGTGTGAACGGGGAAAGCCACATACCAGGAATCACCACCCTCACGGA
CTGCTTGCGGAGGTTTACGAGGCCAGAGCACTTAGGAAGCAGTGCCAAAA
TCAAATGTGGTAGTTGCCAAAGCTACCAGGAATCTACCAAACAGCTCACA
ATGAATAAATTACCTGTCGTTGCCTGTTTTCATTTCAAACGGTTTGAACA
TTCAGCGAAACAGAGGCGCAAGATCACTACATACATTTCCTTTCCTCTGG
AGCTGGATATGACGCCGTTTATGGCCTCAAGTAAAGAGAGCAGAATGAAT
GGACAATTGCAGCTGCCAACCAATAGTGGAAACAACGAAAATAAGTATTC
CTTGTTTGCTGTGGTTAATCACCAAGGAACCTTGGAGAGTGGCCACTATA
CCAGCTTCATCCGGCACCACAAGGACCAGTGGTTCAAGTGTGATGATGCC
GTCATCACTAAGGCCAGTATTAAGGACGTACTGGACAGTGAAGGGTATTT
ACTGTTCTATCACAAACAGGTGCTAGAACATGAGTCAGAAAAAGTGAAAG
AAATGAACACACAAGCCTACTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMCKDYVYDKDIEQIAKEEQGEA
LKLQASTSTEVSHQQCSVPGLGEKFPTWETTKPELELLGHNPRRRRITSS
FTIGLRGLINLGNTCFMNCIVQALTHTPILRDFFLSDRHRCEMPSPELCL
VCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQDAHEFLIAA
LDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGVSTT
IDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTLTDCLRRFTR
PEHLGSSAKIKCGSCQSYQESTKQLTMNKLPVVACFHFKRFEHSAKQRRK
ITTYISFPLELDMTPFMASSKESRMNGQLQLPTNSGNNENKYSLFAVVNH
QGTLESGHYTSFIRHHKDQWFKCDDAVITKASIKDVLDSEGYLLFYHKQV
LEHESEKVKEMNTQAY*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU36343
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; ; Estimated MW of expressed protein : 53000 Daltons