cDNA Clone IRS4

• Synonyms: IRS-4, PY160
• Plasmid: pcDNA5-FRT/TO-FLAG IRS4
• Parent Plasmid: pcDNA5 FRT/TO FLAG
• Expressed: FLAG IRS4
• Genbank: NM_003604.2 GI:224586939 (with silent changes to kill Not1 and BglII)
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCTATGGCGAGTTGCTCCTTCACTCGCGACCAAGCGACAAGAAGACT AAGAGGTGCAGCAGCGGCGGCAGCGGCAGCTCTAGCAGCAGTGGTGACCA CCCCGCTTCTTTCCTCGGGAACCCCGACCGCACTCATTGGGACCGGGTCG TCTTGTCCGGGAGCCATGTGGCTCTCCACGGCCACTGGCTCCCGGTCAGA CTCCGAGTCCGAAGAGGAGGACCTGCCCGTCGGGGAGGAAGTCTGCAAAC GCGGCTACCTGCGGAAACAGAAGCATGGGCACAGGCGCTACTTCGTGCTC AAACTCGAGACTGCTGACGCCCCAGCTCGGCTGGAATACTACGAAAATGC CAGGAAGTTCCGGCACAGTGTCCGCGCCGCGGCGGCTGCAGCAGCGGCTG CTGCCTCTGGCGCCGCGATCCCCCCGCTCATTCCACCGCGGCGCGTGATC ACCCTATACCAGTGCTTTTCCGTGAGCCAGCGAGCAGATGCAAGGTACCG ACACCTCATTGCTCTTTTCACCCAAGACGAATACTTCGCGATGGTGGCCG AGAACGAGTCGGAGCAGGAAAGCTGGTACTTGCTGCTCAGCCGCCTCATC CTCGAGAGCAAGCGCCGCCGCTGCGGCACGCTCGGCGCGCAGCCGGACGG AGAGCCGGCCGCGCTGGCGGCGGCAGCGGCGGCGGAGCCACCCTTCTATA AAGATGTGTGGCAGGTAATAGTCAAACCCAGGGGGCTGGGGCACAGAAAA GAGCTGAGCGGCGTGTTCCGGCTGTGTCTAACCGACGAGGAGGTCGTGTT TGTGAGGCTGAACACCGAAGTGGCCAGCGTGGTCGTCCAGCTCCTGAGCA TCCGTCGCTGTGGACACTCGGAGCAGTATTTCTTCTTGGAAGTAGGCAGG TCCACTGTCATCGGTCCGGGAGAGCTCTGGATGCAGGTCGATGACTGTGT GGTTGCCCAAAACATGCATGAGCTGTTTTTGGAGAAGATGAGAGCCTTGT GTGCAGACGAATACAGAGCCCGCTGCCGCAGCTACAGCATCAGCATCGGC GCCCACCTGTTAACCCTGCTGTCCGCTAGGAGGCACCTGGGCTTGGTGCC GCTCGAGCCGGGAGGCTGGCTCAGAAGGTCCCGCTTTGAGCAGTTTTGCC ACCTCAGGGCCATCGGCGACGGGGAAGACGAGATGCTTTTCACCAGGCGC TTCGTAACACCCAGCGAGCCTGTGGCCCACTCCAGGCGAGGAAGACTGCA CCTGCCCAGAGGGCGCAGGTCAAGGAGAGCGGTTTCAGTGCCGGCCAGCT TTTTTCGCCGCTTAGCACCCAGCCCAGCACGTCCCCGGCACCCTGCAGAA GCCCCGAACAATGGAGCTCGCCTGTCTTCTGAAGTGTCTGGTTCTGGCTC TGGCAACTTTGGGGAGGAAGGCAATCCCCAGGGCAAAGAAGATCAGGAAG GAAGCGGAGGTGACTACATGCCTATGAACAATTGGGGCTCAGGAAATGGC CGGGGCTCAGGAGGTGGCCAGGGCTCAAATGGCCAAGGCTCCAGTAGCCA TAGCTCGGGAGGAAACCAGTGTTCAGGCGAGGGACAGGGATCCCGAGGTG GTCAGGGCTCAAATGGCCAGGGCTCAGGAGGAAACCAGTGCTCTAGAGAT GGCCAGGGCACCGCAGGTGGGCACGGTTCAGGTGGTGGCCAGAGACCTGG AGGTGGGCATGGCTCAGGTGGTGGCCAGGGACCTGGAGATGGCCATGGCT CAGGTGGTGGCAAGAACTCTGGGGGGGGCAAAGGCTCAGGAAGTGGGAAA GGATCCGATGGTGATGGTGAACGTGGAAAATCTCTGAAGAAAAGATCCTA TTTTGGCAAATTAACTCAAAGCAAGCAACAGCAAATGCCACCACCTCCAC CACCTCCTCCTCCACCCCCACCAGCTGGAGGAACTGGTGGAAAAGGGAAG TCTGGGGGAAGATTCAGACTTTATTTTTGTGTTGACAGAGGAGCCACGAA AGAATGCAAAGAAGCCAAAGAAGTGAAAGATGCAGAGATCCCAGAAGGTG CAGCTCGAGGTCCCCACAGAGCCAGAGCTTTTGATGAAGATGAGGATGAC CCATACGTGCCAATGAGGCCAGGGGTGGCCACCCCTCTTGTAAGCTCCAG TGATTATATGCCAATGGCTCCTCAAAATGTCTCTGCTTCAAAAAAGCGCC ACTCTCGATCCCCTTTTGAAGATTCAAGAGGGTACATGATGATGTTTCCC AGAGTGAGCCCACCACCTGCTCCGAGTCCTCCAAAAGCACCTGATACTAA TAAAGAGGATGACTCAAAGGACAATGACAGTGAGAGTGACTACATGTTTA TGGCTCCTGGAGCCGGTGCAATTCCAAAAAACCCCAGAAATCCTCAGGGT GGCTCTTCCTCCAAAAGTTGGAGCTCCTACTTCTCTCTACCAAACCCTTT TCGGAGCTCACCTTTGGGACAGAATGACAACAGTGAGTATGTGCCAATGT TACCTGGAAAGTTCCTGGGGAGGGGCCTAGACAAAGAAGTCTCCTATAAC TGGGACCCCAAAGATGCAGCTTCAAAGCCTTCAGGTGAGGGATCATTCTC AAAGCCTGGAGATGGGGGATCACCTTCAAAGCCTTCAGATCATGAGCCCC CAAAGAATAAAGCTAAGAGACCTAACCGACTTTCTTTTATTACAAAAGGA TATAAAATCAAGCCAAAACCACAAAAGCCCACACATGAGCAGAGAGAAGC TGACAGCTCTAGTGACTACGTCAACATGGACTTCACTAAAAGAGAGAGCA ATACACCAGCTCCCTCTACTCAAGGACTACCAGATTCGTGGGGCATAATT GCTGAACCCAGACAGTCAGCCTTTTCTAATTATGTGAATGTTGAGTTTGG AGTGCCATTTCCAAATCCAGCAAACGACCTCTCAGACCTTTTAAGAGCTA TACCACGTGCCAACCCCTTATCTCTGGACAGTGCTAGGTGGCCACTTCCT CCCCTTCCCCTCAGTGCTACAGGTAGCAATGCTATTGAGGAAGAGGGTGA CTACATTGAAGTAATTTTCAACTCAGCAATGACACCAGCCATGGCTCTTG CTGACAGTGCCATTCGCTATGATGCTGAAACAGGTCGAATCTATGTGGTC GACCCATTTTCTGAGTGCTGTATGGATATTTCTCTCTCCCCCAGCCGATG TTCTGAACCACCACCTGTAGCTAGGCTGCTGCAGGAAGAAGAGCAGGAGA GAAGACGCCCACAAAGCCGTTCTCAAAGTTTCTTTGCAGCAGCCAGAGCC GCTGTCTCTGCTTTTCCAACAGACAGCCTCGAGAGAGACCTTTCCCCATC CTCAGCCCCGGCTGTCGCTTCGGCTGCAGAGCCGACTTTAGCCCTCAGCC AAGTTGTAGCTGCGGCCTCCGCGCTCGCCGCAGCCCCGGGCATCGGCGCA GCAGCCGCAGCTGCTGGATTTGACTCCGCCTCTGCCCGCTGGTTTCAACC TGTTGCTAATGCTGCTGATGCCGAAGCAGTAAGGGGAGCCCAAGACGTTG CCGGTGGCTCGAACCCTGGAGCCCACAACCCATCTGCAAACCTTGCCAGA GGTGATAACCAGGCTGGCGGGGCTGCCGCTGCAGCTGCCGCTCCGGAACC ACCACCTCGCAGTCGCCGGGTGCCAAGACCCCCGGAGAGAGAAGATTCTG ACAACGACGACGACACTCACGTGAGAATGGATTTTGCCAGACGTGATAAT CAGTTCGACTCTCCCAAAAGAGGTCGGTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMASCSFTRDQATRRLRGAAAAAAAALAAVVTTPLLSSGT
PTALIGTGSSCPGAMWLSTATGSRSDSESEEEDLPVGEEVCKRGYLRKQK
HGHRRYFVLKLETADAPARLEYYENARKFRHSVRAAAAAAAAAASGAAIP
PLIPPRRVITLYQCFSVSQRADARYRHLIALFTQDEYFAMVAENESEQES
WYLLLSRLILESKRRRCGTLGAQPDGEPAALAAAAAAEPPFYKDVWQVIV
KPRGLGHRKELSGVFRLCLTDEEVVFVRLNTEVASVVVQLLSIRRCGHSE
QYFFLEVGRSTVIGPGELWMQVDDCVVAQNMHELFLEKMRALCADEYRAR
CRSYSISIGAHLLTLLSARRHLGLVPLEPGGWLRRSRFEQFCHLRAIGDG
EDEMLFTRRFVTPSEPVAHSRRGRLHLPRGRRSRRAVSVPASFFRRLAPS
PARPRHPAEAPNNGARLSSEVSGSGSGNFGEEGNPQGKEDQEGSGGDYMP
MNNWGSGNGRGSGGGQGSNGQGSSSHSSGGNQCSGEGQGSRGGQGSNGQG
SGGNQCSRDGQGTAGGHGSGGGQRPGGGHGSGGGQGPGDGHGSGGGKNSG
GGKGSGSGKGSDGDGERGKSLKKRSYFGKLTQSKQQQMPPPPPPPPPPPP
AGGTGGKGKSGGRFRLYFCVDRGATKECKEAKEVKDAEIPEGAARGPHRA
RAFDEDEDDPYVPMRPGVATPLVSSSDYMPMAPQNVSASKKRHSRSPFED
SRGYMMMFPRVSPPPAPSPPKAPDTNKEDDSKDNDSESDYMFMAPGAGAI
PKNPRNPQGGSSSKSWSSYFSLPNPFRSSPLGQNDNSEYVPMLPGKFLGR
GLDKEVSYNWDPKDAASKPSGEGSFSKPGDGGSPSKPSDHEPPKNKAKRP
NRLSFITKGYKIKPKPQKPTHEQREADSSSDYVNMDFTKRESNTPAPSTQ
GLPDSWGIIAEPRQSAFSNYVNVEFGVPFPNPANDLSDLLRAIPRANPLS
LDSARWPLPPLPLSATGSNAIEEEGDYIEVIFNSAMTPAMALADSAIRYD
AETGRIYVVDPFSECCMDISLSPSRCSEPPPVARLLQEEEQERRRPQSRS
QSFFAAARAAVSAFPTDSLERDLSPSSAPAVASAAEPTLALSQVVAAASA
LAAAPGIGAAAAAAGFDSASARWFQPVANAADAEAVRGAQDVAGGSNPGA
HNPSANLARGDNQAGGAAAAAAAPEPPPRSRRVPRPPEREDSDNDDDTHV
RMDFARRDNQFDSPKRGR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36186
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee