cDNA Clone MUSK

• Plasmid: pGEX6P-2-MUSK isoform 2
• Parent Plasmid: pGEX6P2
• Expressed: GST-MUSK isoform 2
• Genbank: NM_001166280.1 GI:261878454*
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGAGAGCTCGTCAACATTCCACTGGTACATATTCTTAC
TCTGGTTGCCTTCAGCGGAACTGAGAAACTTCCAAAAGCTCCTGTCATCA
CCACTCCTCTTGAAACAGTGGATGCCTTAGTTGAAGAAGTGGCTACTTTC
ATGTGTGCAGTGGAATCCTACCCCCAGCCTGAGATTTCCTGGACTAGAAA
TAAAATTCTCATTAAACTCTTTGACACCCGGTACAGCATCCGGGAGAATG
GGCAGCTCCTCACCATCCTGAGTGTGGAAGACAGTGATGATGGCATTTAC
TGCTGCACGGCCAACAATGGTGTGGGAGGAGCTGTGGAGAGTTGTGGAGC
CCTGCAAGTGAAGATGAAACCTAAAATAACTCGTCCTCCCATAAATGTGA
AAATAATAGAAGGATTAAAAGCAGTCCTACCATGTACTACAATGGGTAAT
CCCAAACCATCAGTGTCTTGGATAAAGGGAGACAGCCCTCTCAGGGAAAA
TTCCCGAATTGCAGTTCTTGAATCTGGGAGCTTGAGGATTCATAACGTAC
AAAAGGAAGATGCAGGACAGTATCGATGTGTGGCAAAAAACAGCCTCGGG
ACAGCATATTCCAAAGTGGTGAAGCTGGAAGTTGAGGAAGAAAGTGAACC
CGAACAAGATACTAAAGTTTTTGCCAGGATCCTGCGGGCTCCTGAATCCC
ACAATGTCACCTTTGGCTCCTTTGTGACCCTGCACTGTACAGCAACAGGC
ATTCCTGTCCCCACCATCACCTGGATTGAAAACGGAAATGCTGTTTCTTC
TGGGTCCATTCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTGC
AGCTGTTTATCACCAAGCCAGGACTCTACACATGCATAGCTACCAATAAG
CATGGGGAGAAGTTCAGTACTGCCAAGGCTGCAGCCACCATCAGCATAGC
AGAATGGAGAGAGTACTGCTTGGCAGTAAAGGAGCTCTTCTGCGCAAAAG
AATGGCTGGTAATGGAAGAGAAGACCCACAGAGGACTCTACAGATCCGAG
ATGCATTTGCTGTCCGTGCCAGAATGCAGCAAGCTTCCCAGCATGCATTG
GGACCCCACGGCCTGTGCCAGACTGCCACATCTAGCATTCCCACCAATGA
CGTCCTCAAAGCCAAGTGTGGACATTCCAAATCTGCCTTCCTCCTCCTCT
TCTTCCTTCTCTGTCTCACCTACATACTCCATGACTGTAATAATCTCCAT
CATGTCCAGCTTTGCAATATTTGTGCTTCTTACCATAACTACTCTCTATT
GCTGCCGAAGAAGAAAACAATGGAAAAATAAGAAAAGAGAATCAGCAGCA
GTAACCCTCACCACACTGCCTTCTGAGCTCTTACTAGATAGACTTCATCC
CAACCCCATGTACCAGAGGATGCCGCTCCTTCTGAACCCCAAATTGCTCA
GCCTGGAGTATCCAAGGAATAACATTGAATATGTGAGAGACATCGGAGAG
GGAGCGTTTGGAAGGGTGTTTCAAGCAAGGGCACCAGGCTTACTTCCCTA
TGAACCTTTCACTATGGTGGCAGTAAAGATGCTCAAAGAAGAAGCCTCGG
CAGATATGCAAGCGGACTTTCAGAGGGAGGCAGCCCTCATGGCAGAATTT
GACAACCCTAACATTGTGAAGCTATTAGGAGTGTGTGCTGTCGGGAAGCC
AATGTGCCTGCTCTTTGAATACATGGCCTATGGTGACCTCAATGAGTTCC
TCCGCAGCATGTCCCCTCACACCGTGTGCAGCCTCAGTCACAGTGACTTG
TCTATGAGGGCTCAGGTCTCCAGCCCTGGGCCCCCACCCCTCTCCTGTGC
TGAGCAGCTTTGCATTGCCAGGCAGGTGGCAGCTGGCATGGCTTACCTCT
CAGAACGTAAGTTTGTTCACCGAGATTTAGCCACCAGGAACTGCCTGGTG
GGCGAGAACATGGTGGTGAAAATTGCCGACTTTGGCCTCTCCAGGAACAT
CTACTCAGCAGACTACTACAAAGCTAATGAAAACGACGCTATCCCTATCC
GTTGGATGCCACCAGAGTCCATTTTTTATAACCGCTACACTACAGAGTCT
GATGTGTGGGCCTATGGCGTGGTCCTCTGGGAGATCTTCTCCTATGGCCT
GCAGCCCTACTATGGGATGGCCCATGAGGAGGTCATTTACTACGTGCGAG
ATGGCAACATCCTCTCCTGCCCTGAGAACTGCCCCGTGGAGCTGTACAAT
CTCATGCGTCTATGTTGGAGCAAGCTGCCTGCAGACAGACCCAGTTTCAC
CAGTATTCACCGAATTCTGGAACGCATGTGTGAGAGGGCAGAGGGAACTG
TGAGTGTCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMRELVNIP
LVHILTLVAFSGTEKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPE
ISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCTANNGVGGA
VESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGD
SPLRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKVVKLEV
EEESEPEQDTKVFARILRAPESHNVTFGSFVTLHCTATGIPVPTITWIEN
GNAVSSGSIQESVKDRVIDSRLQLFITKPGLYTCIATNKHGEKFSTAKAA
ATISIAEWREYCLAVKELFCAKEWLVMEEKTHRGLYRSEMHLLSVPECSK
LPSMHWDPTACARLPHLAFPPMTSSKPSVDIPNLPSSSSSSFSVSPTYSM
TVIISIMSSFAIFVLLTITTLYCCRRRKQWKNKKRESAAVTLTTLPSELL
LDRLHPNPMYQRMPLLLNPKLLSLEYPRNNIEYVRDIGEGAFGRVFQARA
PGLLPYEPFTMVAVKMLKEEASADMQADFQREAALMAEFDNPNIVKLLGV
CAVGKPMCLLFEYMAYGDLNEFLRSMSPHTVCSLSHSDLSMRAQVSSPGP
PPLSCAEQLCIARQVAAGMAYLSERKFVHRDLATRNCLVGENMVVKIADF
GLSRNIYSADYYKANENDAIPIRWMPPESIFYNRYTTESDVWAYGVVLWE
IFSYGLQPYYGMAHEEVIYYVRDGNILSCPENCPVELYNLMRLCWSKLPA
DRPSFTSIHRILERMCERAEGTVSV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36160
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_001166280.1 CDS:; g402a (silent); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 115397 Daltons