cDNA Clone EPHA7

• Synonyms: EHK3, HEK11
• Plasmid: pGEX6P-3-EPHA7
• Parent Plasmid: pGEX6P3
• Expressed: GST-EPHA7
• Genbank: NM_004440.3 GI:205277372*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGTTTTTCAAACTCGGTACCCTTCATGGATTATTTTATGCTA
CATCTGGCTGCTCCGCTTTGCACACACAGGGGAGGCGCAGGCTGCGAAGG
AAGTACTACTGCTGGATTCTAAAGCACAACAAACAGAGTTGGAGTGGATT
TCCTCTCCACCCAATGGGTGGGAAGAAATTAGTGGTTTGGATGAGAACTA
TACCCCGATACGAACATACCAGGTGTGCCAAGTCATGGAGCCCAACCAAA
ACAACTGGCTGCGGACTAACTGGATTTCCAAAGGCAATGCACAAAGGATT
TTTGTAGAATTGAAATTCACCCTGAGGGATTGTAACAGTCTTCCTGGAGT
ACTGGGAACTTGCAAGGAAACATTTAATTTGTACTATTATGAAACAGACT
ATGACACTGGCAGGAATATAAGAGAAAACCTCTATGTAAAAATAGACACC
ATTGCTGCAGATGAAAGTTTTACCCAAGGTGACCTTGGTGAAAGAAAGAT
GAAGCTTAACACTGAGGTGAGAGAGATTGGACCTTTGTCCAAAAAGGGAT
TCTATCTTGCCTTTCAGGATGTAGGGGCTTGCATAGCTTTGGTTTCTGTC
AAAGTGTACTACAAGAAGTGCTGGTCCATTATTGAGAACTTAGCTATCTT
TCCAGATACAGTGACTGGTTCAGAATTTTCCTCTTTAGTCGAGGTTCGAG
GGACATGTGTCAGCAGTGCAGAGGAAGAAGCGGAAAACGCCCCCAGGATG
CACTGCAGTGCAGAAGGAGAATGGTTAGTGCCCATTGGAAAATGTATCTG
CAAAGCAGGCTACCAGCAAAAAGGAGACACTTGTGAACCCTGTGGCCGTG
GGTTCTACAAGTCTTCCTCTCAAGATCTTCAGTGCTCTCGTTGTCCAACT
CACAGTTTTTCTGATAAAGAAGGCTCCTCCAGATGTGAATGTGAAGATGG
GTATTACAGGGCTCCATCTGACCCACCATACGTTGCATGCACAAGGCCTC
CATCTGCACCACAGAACCTCATTTTCAACATCAACCAAACCACAGTAAGT
TTGGAATGGAGTCCTCCTGCAGACAATGGGGGAAGAAACGATGTGACCTA
CAGAATATTGTGTAAGCGGTGCAGTTGGGAGCAGGGCGAATGTGTTCCCT
GTGGGAGTAACATTGGATACATGCCCCAGCAGACTGGATTAGAGGATAAC
TATGTCACTGTCATGGACCTGCTAGCCCACGCTAATTATACTTTTGAAGT
TGAAGCTGTAAATGGAGTTTCTGACTTAAGCCGATCCCAGAGGCTCTTTG
CTGCTGTCAGTATCACCACTGGTCAAGCAGCTCCCTCGCAAGTGAGCGGA
GTAATGAAGGAGAGAGTACTGCAGCGGAGTGTCGAGCTTTCCTGGCAGGA
ACCAGAGCATCCCAATGGAGTCATCACAGAATATGAAATCAAGTATTACG
AGAAAGATCAAAGGGAACGGACCTACTCAACAGTAAAAACCAAGTCTACT
TCAGCCTCCATTAATAATCTGAAACCAGGAACAGTGTATGTTTTCCAGAT
TCGGGCTTTTACTGCTGCTGGTTATGGAAATTACAGTCCCAGACTTGATG
TTGCTACACTAGAGGAAGCTACAGGTAAAATGTTTGAAGCTACAGCTGTC
TCCAGTGAACAGAATCCTGTTATTATCATTGCTGTGGTTGCTGTAGCTGG
GACCATCATTTTGGTGTTCATGGTCTTTGGCTTCATCATTGGGAGAAGGC
ACTGTGGTTATAGCAAAGCTGACCAAGAAGGCGATGAAGAGCTTTACTTT
CATTTTAAATTTCCAGGCACCAAAACCTACATTGACCCTGAAACCTATGA
GGACCCAAATAGAGCTGTCCATCAATTCGCCAAGGAGCTAGATGCCTCCT
GTATTAAAATTGAGCGTGTGATTGGTGCAGGAGAATTCGGTGAAGTCTGC
AGTGGCCGTTTGAAACTTCCAGGGAAAAGAGATGTTGCAGTAGCCATAAA
AACCCTGAAAGTTGGTTACACAGAAAAACAAAGGAGAGACTTTTTGTGTG
AAGCAAGCATCATGGGGCAGTTTGACCACCCGAATGTTGTCCATTTGGAA
GGGGTTGTTACAAGAGGGAAACCAGTCATGATAGTAATAGAGTTCATGGA
AAATGGAGCCCTAGATGCATTTCTCAGGAAACATGATGGGCAATTTACAG
TCATTCAGTTAGTAGGAATGCTGAGAGGAATTGCTGCTGGAATGAGATAT
TTGGCTGATATGGGATATGTTCACAGGGACCTTGCAGCTCGCAATATTCT
TGTCAACAGCAATCTCGTTTGTAAAGTGTCAGATTTTGGCCTGTCCCGAG
TTATAGAGGATGATCCAGAAGCTGTCTATACAACTACTGGTGGAAAAATT
CCAGTAAGGTGGACAGCACCCGAAGCCATCCAGTACCGGAAATTCACATC
AGCCAGTGATGTATGGAGCTATGGAATAGTCATGTGGGAAGTTATGTCTT
ATGGAGAAAGACCTTATTGGGACATGTCAAATCAAGATGTTATAAAAGCA
ATAGAAGAAGGTTATCGTTTACCAGCACCCATGGACTGCCCAGCTGGCCT
TCACCAGCTAATGTTGGATTGTTGGCAAAAGGAGCGTGCTGAAAGGCCAA
AATTTGAACAGATAGTTGGAATTCTAGACAAAATGATTCGAAACCCAAAT
AGTCTGAAAACTCCCCTGGGAACTTGTAGTAGGCCAATAAGCCCTCTTCT
GGATCAAAACACTCCTGATTTCACTACCTTTTGTTCAGTTGGAGAATGGC
TACAAGCTATTAAGATGGAAAGATATAAAGATAATTTCACGGCAGCTGGC
TACAATTCCCTTGAATCAGTAGCCAGGATGACTATTGAGGATGTGATGAG
TTTAGGGATCACACTGGTTGGTCATCAAAAGAAAATCATGAGCAGCATTC
AGACTATGAGAGCACAAATGCTACATTTACATGGAACTGGCATTCAAGTG
TGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVFQTRYPSWIILCYIWLL
RFAHTGEAQAAKEVLLLDSKAQQTELEWISSPPNGWEEISGLDENYTPIR
TYQVCQVMEPNQNNWLRTNWISKGNAQRIFVELKFTLRDCNSLPGVLGTC
KETFNLYYYETDYDTGRNIRENLYVKIDTIAADESFTQGDLGERKMKLNT
EVREIGPLSKKGFYLAFQDVGACIALVSVKVYYKKCWSIIENLAIFPDTV
TGSEFSSLVEVRGTCVSSAEEEAENAPRMHCSAEGEWLVPIGKCICKAGY
QQKGDTCEPCGRGFYKSSSQDLQCSRCPTHSFSDKEGSSRCECEDGYYRA
PSDPPYVACTRPPSAPQNLIFNINQTTVSLEWSPPADNGGRNDVTYRILC
KRCSWEQGECVPCGSNIGYMPQQTGLEDNYVTVMDLLAHANYTFEVEAVN
GVSDLSRSQRLFAAVSITTGQAAPSQVSGVMKERVLQRSVELSWQEPEHP
NGVITEYEIKYYEKDQRERTYSTVKTKSTSASINNLKPGTVYVFQIRAFT
AAGYGNYSPRLDVATLEEATGKMFEATAVSSEQNPVIIIAVVAVAGTIIL
VFMVFGFIIGRRHCGYSKADQEGDEELYFHFKFPGTKTYIDPETYEDPNR
AVHQFAKELDASCIKIERVIGAGEFGEVCSGRLKLPGKRDVAVAIKTLKV
GYTEKQRRDFLCEASIMGQFDHPNVVHLEGVVTRGKPVMIVIEFMENGAL
DAFLRKHDGQFTVIQLVGMLRGIAAGMRYLADMGYVHRDLAARNILVNSN
LVCKVSDFGLSRVIEDDPEAVYTTTGGKIPVRWTAPEAIQYRKFTSASDV
WSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPAPMDCPAGLHQLM
LDCWQKERAERPKFEQIVGILDKMIRNPNSLKTPLGTCSRPISPLLDQNT
PDFTTFCSVGEWLQAIKMERYKDNFTAAGYNSLESVARMTIEDVMSLGIT
LVGHQKKIMSSIQTMRAQMLHLHGTGIQV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36154
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_004440.3 CDS:; g981a, t1341c both silent; Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 138916 Daltons