cDNA Clone DCLK2 (DCSMKL2)

• Synonyms: CL2, CLICK-II, CLICK2, CLIK2, DCAMKL2, DCDC3, DCDC3B, DCK2, DKFZp761I032, MGC45428
• Plasmid: pGEX6P-2-DCLK2 (DCSMKL2)
• Parent Plasmid: pGEX6P2
• Expressed: GST-DCLK2 (DCSMKL2)
• Genbank: NM_152619.1 GI:22749266
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCCAGCACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAAGGCCGCGGCCGGGGTCGCGGAGAGGGGCCCCCAGCTCCTCCGGGGGCAGCAGCAGCTCGGGCCCCAAGGGGAACGGGCTCATCCCCAGTCCGGCGCACAGTGCCCACTGCAGCTTCTACCGCACGCGGACCCTGCAGGCCCTCAGCTCGGAGAAGAAGGCCAAGAAGGCGCGCTTCTACCGGAACGGGGACCGCTACTTCAAGGGCCTGGTGTTTGCCATCTCCAGCGACCGCTTCCGGTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCCCTGTCGGACAACGTGAACCTGCCCCAGGGTGTCCGCACTATCTACACCATCGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAAGGTGAGAGTTACGTGTGTGCATCCAATGAA
CCATTTCGTAAAGTCGATTACACCAAAAATATTAATCCAAACTGGTCTGT
GAACATCAAGGGTGGGACATCCCGAGCGCTGGCTGCTGCCTCCTCTGTGA
AAAGTGAAGTAAAAGAAAGTAAAGATTTCATCAAACCCAAGTTAGTGACT
GTGATTCGAAGTGGAGTGAAGCCTAGAAAAGCCGTGCGGATCCTTCTGAA
TAAAAAGACTGCTCATTCCTTTGAACAAGTCTTAACAGATATCACCGAAG
CCATTAAACTAGACTCAGGAGTCGTCAAGAGGCTCTGCACCCTGGATGGA
AAGCAGGTTACTTGTCTGCAAGACTTTTTTGGTGATGACGATGTTTTTAT
TGCATGTGGACCAGAAAAATTTCGTTATGCCCAAGATGACTTTGTCCTGG
ATCATAGTGAATGTCGTGTCCTGAAGTCATCTTATTCTCGATCCTCAGCT
GTTAAGTATTCTGGATCCAAAAGCCCTGGGCCCTCTCGACGCAGCAAATC
ACCAGCTTCAGTTAATGGAACTCCCAGCAGCCAACTTTCTACTCCTAAAT
CTACGAAATCCTCCAGTTCCTCTCCAACTAGTCCAGGAAGTTTCAGAGGA
TTAAAGATTTCTGCTCATGGCAGATCTTCTTCCAATGTAAACGGTGGACC
TGAGCTTGACCGTTGCATAAGTCCTGAAGGTGTGAATGGAAACAGATGCT
CTGAATCATCAACTCTTCTTGAGAAATACAAAATTGGAAAGGTCATTGGT
GATGGCAATTTTGCAGTAGTCAAAGAGTGTATAGACAGGTCCACTGGAAA
GGAGTTTGCCCTAAAGATTATAGACAAAGCCAAATGTTGTGGAAAGGAAC
ACCTGATTGAGAATGAAGTGTCAATACTGCGCCGAGTGAAACATCCCAAT
ATCATTATGCTGGTCGAGGAGATGGAAACAGCAACTGAGCTCTTTCTGGT
GATGGAATTGGTCAAAGGTGGAGATCTCTTTGATGCAATTACTTCGTCGA
CCAAGTACACTGAGAGAGATGGCAGTGCCATGGTGTACAACTTAGCCAAT
GCCCTCAGGTATCTCCATGGCCTCAGCATCGTGCACAGAGACATCAAACC
AGAGAATCTCTTGGTGTGTGAATATCCTGATGGAACCAAGTCTTTGAAAC
TGGGAGACTTTGGGCTTGCGACTGTGGTAGAAGGCCCTTTATACACAGTC
TGTGGCACACCCACTTATGTGGCTCCAGAAATCATTGCTGAAACTGGCTA
TGGCCTGAAGGTGGACATTTGGGCAGCTGGTGTGATCACATACATACTTC
TCTGTGGATTCCCACCATTCCGAAGTGAGAACAATCTCCAGGAAGATCTC
TTCGACCAGATCTTGGCTGGGAAGCTGGAGTTTCCGGCCCCCTACTGGGA
TAACATCACGGACTCTGCCAAGGAATTAATCAGTCAAATGCTTCAGGTAA
ATGTTGAAGCTCGGTGTACCGCGGGACAAATCCTGAGTCACCCCTGGGTG
TCAGATGATGCCTCCCAGGAGAATAACATGCAAGCTGAGGTGACAGGTAA
ACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACTACCA
CCGGGGTCTCCGTCATCATGTTTGATTTGACAGTTTGAGAGGCACCTCAG
AGTTTCCTTCTTTACCCCGCTGAGACAGTGGGAGAGCCTGAAGCAGAATC
CTGAACACGGCTCTAGATAAGGAGGGGCAGATTTTCTGCAGCAAGCACTG
TCAAGACAGCGGCAGGCCTGGGATGGAGCCCATCTCTCCAGTTCCTCCCT
CAGTGGAGGAGATCCCTGTGCCTGGGGAAGCAGTCCCGGCCCCCACCCCT
CCGGAATCTCCCACCCCCCACTGTCCTCCCGCTGCCCCGGGTGGTGAGCG
GGCAGGAACCTGGCGCCGCCACCGAGACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMASTRSIE
LEHFEERDKRPRPGSRRGAPSSSGGSSSSGPKGNGLIPSPAHSAHCSFYR
TRTLQALSSEKKAKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTR
SLSDNVNLPQGVRTIYTIDGSRKVTSLDELLEGESYVCASNEPFRKVDYT
KNINPNWSVNIKGGTSRALAAASSVKSEVKESKDFIKPKLVTVIRSGVKP
RKAVRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQD
FFGDDDVFIACGPEKFRYAQDDFVLDHSECRVLKSSYSRSSAVKYSGSKS
PGPSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGLKISAHGR
SSSNVNGGPELDRCISPEGVNGNRCSESSTLLEKYKIGKVIGDGNFAVVK
ECIDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEM
ETATELFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHGL
SIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVA
PEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGK
LEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWVSDDASQEN
NMQAEVTGKLKQHFNNALPKQNSTTTGVSVIMFDLTV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36124
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 103948 Daltons