cDNA Clone GRK4

• Synonyms: GPRK2L, GPRK4, GRK4a, IT11
• Plasmid: pGEX6P-3-GRK4
• Parent Plasmid: pGEX6P3
• Expressed: GST-GRK4
• Genbank: NM_182982.2 GI:51873042*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGCTCGAGAACATCGTGGCCAACTCGCTGCTGCTGAAAGC
GCGTCAAGGAGGATATGGCAAAAAAAGTGGTCGTAGTAAAAAATGGAAGG
AGATACTGACACTGCCTCCTGTCAGCCAGTGCAGTGAGCTTAGACATTCC
ATTGAAAAGGATTATAGCAGTCTTTGTGACAAGCAACCGATAGGAAGACG
TCTCTTCAGGCAGTTCTGTGATACCAAACCCACTCTAAAGAGGCACATTG
AATTCTTGGATGCAGTGGCAGAATATGAAGTTGCCGATGATGAGGACCGA
AGTGATTGTGGACTGTCAATCTTAGATAGATTCTTCAATGATAAGTTGGC
AGCCCCTTTACCAGAAATACCTCCAGATGTTGTGACAGAATGTAGATTGG
GACTGAAGGAGGAGAACCCTTCCAAAAAAGCCTTTGAGGAATGTACTAGA
GTTGCCCATAACTACCTAAGAGGGGAACCATTTGAAGAATACCAAGAAAG
CTCATATTTTTCTCAGTTTTTACAATGGAAATGGCTGGAAAGGCAACCCG
TAACAAAGAACACATTTAGACATTACAGAGTTCTAGGAAAAGGCGGATTT
GGAGAGGTTTGCGCCTGTCAAGTGCGAGCCACAGGAAAAATGTATGCCTG
CAAAAAGCTACAAAAAAAAAGAATAAAGAAGAGGAAAGGTGAAGCTATGG
CTCTAAATGAGAAAAGAATTCTGGAGAAAGTGCAAAGTAGATTCGTAGTT
AGTTTAGCCTACGCTTATGAAACCAAAGATGCCTTGTGCTTGGTGCTCAC
CATTATGAATGGAGGGGATTTGAAGTTTCACATTTACAACCTGGGCAATC
CCGGCTTTGATGAGCAGAGAGCCGTTTTCTATGCTGCAGAGCTGTGTTGC
GGCTTGGAAGATTTACAGAGGGAAAGAATTGTATACAGAGACTTGAAGCC
TGAGAATATTCTCCTTGATGATCGTGGACACATCCGGATTTCAGACCTCG
GTTTGGCCACAGAGATCCCAGAAGGACAGAGGGTTCGAGGAAGAGTTGGA
ACAGTCGGCTACATGGCACCTGAAGTTGTCAATAATGAAAAGTATACGTT
TAGTCCCGATTGGTGGGGACTTGGCTGTCTGATCTATGAAATGATTCAGG
GACATTCTCCATTCAAAAAATACAAAGAGAAAGTCAAATGGGAGGAGGTC
GATCAAAGAATCAAGAATGATACCGAGGAGTATTCTGAGAAGTTTTCAGA
GGATGCCAAATCTATCTGCAGGATGTTACTCACCAAGAATCCAAGCAAGC
GGCTGGGCTGCAGGGGCGAGGGAGCGGCTGGGGTGAAGCAGCACCCCGTG
TTCAAGGACATCAACTTCAGGAGGCTGGAGGCAAACATGCTGGAGCCCCC
TTTCTGTCCTGATCCTCATGCCGTTTACTGTAAGGACGTCCTGGATATCG
AGCAGTTCTCGGCGGTGAAAGGGATCTACCTGGACACCGCAGATGAAGAC
TTCTATGCTCGGTTTGCTACCGGGTGTGTCTCCATCCCCTGGCAGAATGA
GATGATCGAATCTGGGTGTTTCAAAGACATCAACAAAAGTGAAAGTGAGG
AAGCTTTGCCATTAGATCTAGACAAGAACATACATACCCCGGTTTCCAGA
CCAAACAGAGGCTTCTTCTATAGACTCTTCAGAAGAGGGGGCTGCCTGAC
CATGGTCCCCAGTGAGAAGGAAGTGGAACCCAAGCAATGCTGAGCGGCCG
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMELENIVANSLLLKARQGG
YGKKSGRSKKWKEILTLPPVSQCSELRHSIEKDYSSLCDKQPIGRRLFRQ
FCDTKPTLKRHIEFLDAVAEYEVADDEDRSDCGLSILDRFFNDKLAAPLP
EIPPDVVTECRLGLKEENPSKKAFEECTRVAHNYLRGEPFEEYQESSYFS
QFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLQ
KKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNG
GDLKFHIYNLGNPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENIL
LDDRGHIRISDLGLATEIPEGQRVRGRVGTVGYMAPEVVNNEKYTFSPDW
WGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKS
ICRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDINFRRLEANMLEPPFCPD
PHAVYCKDVLDIEQFSAVKGIYLDTADEDFYARFATGCVSIPWQNEMIES
GCFKDINKSESEEALPLDLDKNIHTPVSRPNRGFFYRLFRRGGCLTMVPS
EKEVEPKQC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_182982.2 CDS:; t1457c (V486A) - present in many published sequences; Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 93377 Daltons