cDNA Clone GRK5

• Synonyms: GPRK5
• Plasmid: pGEX6P-2-GRK5
• Parent Plasmid: pGEX6P2
• Expressed: GST-GRK5
• Genbank: NM_005308.2 GI:51896033
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGC
CAGGGAAGGGGGCGGAGGAAAGCGCAAAGGGAAAAGCAAGAAGTGGAAAG
AAATCCTGAAGTTCCCTCACATTAGCCAGTGTGAAGACCTCCGAAGGACC
ATAGACAGAGATTACTGCAGTTTATGTGACAAGCAGCCAATCGGGAGGCT
GCTTTTCCGGCAGTTTTGTGAAACCAGGCCTGGGCTGGAGTGTTACATTC
AGTTCCTGGACTCCGTGGCAGAATATGAAGTTACTCCAGATGAAAAACTG
GGAGAGAAAGGGAAGGAAATTATGACCAAGTACCTCACCCCAAAGTCCCC
TGTTTTCATAGCCCAAGTTGGCCAAGACCTGGTCTCCCAGACGGAGGAGA
AGCTCCTACAGAAGCCGTGCAAAGAACTCTTTTCTGCCTGTGCACAGTCT
GTCCACGAGTACCTGAGGGGAGAACCATTCCACGAATATCTGGACAGCAT
GTTTTTTGACCGCTTTCTCCAGTGGAAGTGGTTGGAAAGGCAACCGGTGA
CCAAAAACACTTTCAGGCAGTATCGAGTGCTAGGAAAAGGGGGCTTCGGG
GAGGTCTGTGCCTGCCAGGTTCGGGCCACGGGTAAAATGTATGCCTGCAA
GCGCTTGGAGAAGAAGAGGATCAAAAAGAGGAAAGGGGAGTCCATGGCCC
TCAATGAGAAGCAGATCCTCGAGAAGGTCAACAGTCAGTTTGTGGTCAAC
CTGGCCTATGCCTACGAGACCAAGGATGCACTGTGCTTGGTCCTGACCAT
CATGAATGGGGGTGACCTGAAGTTCCACATCTACAACATGGGCAACCCTG
GCTTCGAGGAGGAGCGGGCCTTGTTTTATGCGGCAGAGATCCTCTGCGGC
TTAGAAGACCTCCACCGTGAGAACACCGTCTACCGAGATCTGAAACCTGA
AAACATCCTGTTAGATGATTATGGCCACATTAGGATCTCAGACCTGGGCT
TGGCTGTGAAGATCCCCGAGGGAGACCTGATCCGCGGCCGGGTGGGCACT
GTTGGCTACATGGCTCCAGAGGTCCTGAACAACCAGAGGTACGGCCTGAG
CCCCGACTACTGGGGCCTTGGCTGCCTCATCTATGAGATGATCGAGGGCC
AGTCGCCGTTCCGCGGCCGCAAGGAGAAGGTGAAGCGGGAGGAGGTGGAC
CGCCGGGTCCTGGAGACGGAGGAGGTGTACTCCCACAAGTTCTCCGAGGA
GGCCAAGTCCATCTGCAAGATGCTGCTCACGAAAGATGCGAAGCAGAGGC
TGGGCTGCCAGGAGGAGGGGGCTGCAGAGGTCAAGAGACACCCCTTCTTC
AGGAACATGAACTTCAAGCGCTTAGAAGCCGGGATGTTGGACCCTCCCTT
CGTTCCAGACCCCCGCGCTGTGTACTGTAAGGACGTGCTGGACATCGAGC
AGTTCTCCACTGTGAAGGGCGTCAATCTGGACCACACAGACGACGACTTC
TACTCCAAGTTCTCCACGGGCTCTGTGTCCATCCCATGGCAAAACGAGAT
GATAGAAACAGAATGCTTTAAGGAGCTGAACGTGTTTGGACCTAATGGTA
CCCTCCCGCCAGATCTGAACAGAAACCACCCTCCGGAACCGCCCAAGAAA
GGGCTGCTCCAGAGACTCTTCAAGCGGCAGCATCAGAACAATTCCAAGAG
TTCGCCCAGCTCCAAGACCAGTTTTAACCACCACATAAACTCAAACCATG
TCAGCTCGAACTCCACCGGAAGCAGCTAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMELENIVANTVLLKAREGG
GGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYCSLCDKQPIGRLLFRQ
FCETRPGLECYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFIA
QVGQDLVSQTEEKLLQKPCKELFSACAQSVHEYLRGEPFHEYLDSMFFDR
FLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEK
KRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGG
DLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILL
DDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYW
GLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSI
CKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDP
RAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETE
CFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSS
KTSFNHHINSNHVSSNSTGSS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36101
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 94608 Daltons