cDNA Clone PBK

• Synonyms: CT84, FLJ14385, Nori-3, SPK, TOPK
• Plasmid: pGEX6P-3-PBK
• Parent Plasmid: pGEX6P3
• Expressed: GST-PBK
• Genbank: NM_018492.2 GI:18490990*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAGGGATCAGTAATTTCAAGACACCAAGCAAATTATCAGA
AAAAAAGAAATCTGTATTATGTTCAACTCCAACTATAAATATCCCGGCCT
CTCCATTTATGCAGAAGCTTGGCTTTGGTACTGGGGTAAATGTGTACCTA
ATGAAAAGATCTCCAAGAGGTTTGTCTCATTCTCCTTGGGCTGTAAAAAA
GATTAATCCTATATGTAATGATCATTATCGAAGTGTGTATCAAAAGAGAC
TAATGGATGAAGCTAAGATTTTGAAAAGCCTTCATCATCCAAACATTGTT
GGTTATCGTGCTTTTACTGAAGCCAATGATGGCAGTCTGTGTCTTGCTAT
GGAATATGGAGGTGAAAAGTCTCTAAATGACTTAATAGAAGAACGATATA
AAGCCAGCCAAGATCCTTTTCCAGCAGCCATAATTTTAAAAGTTGCTTTG
AATATGGCAAGAGGGTTAAAGTATCTGCACCAAGAAAAGAAACTGCTTCA
TGGAGACATAAAGTCTTCAAATGTTGTAATTAAAGGCGATTTTGAAACAA
TTAAAATCTGTGATGTAGGAGTCTCTCTACCACTGGATGAAAATATGACT
GTGACTGACCCTGAGGCTTGTTACATTGGCACAGAGCCATGGAAACCCAA
AGAAGCTGTGGAGGAGAATGGTGTTATTACTGACAAGGCAGACATATTTG
CCTTTGGCCTTACTTTGTGGGAAATGATGACTTTATCGATTCCACACATT
AATCTTTCAAATGATGATGATGATGAAGATAAAACTTTTGATGAAAGTGA
TTTTGATGATGAAGCATACTATGCAGCGTTGGGAACTAGGCCACCTATTA
ATATGGAAGAACTGGATGAATCATACCAGAAAGTAATTGAACTCTTCTCT
GTATGCACTAATGAAGACCCTAAAGATCGTCCTTCTGCTGCACACATTGT
TGAAGCTCTGGAAACAGATGTCTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEGISNFKTPSKLSEKKKS
VLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPI
CNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGG
EKSLNDLIEERYKASQDPFPAAIILKVALNMARGLKYLHQEKKLLHGDIK
SSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVE
ENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDE
AYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALE
TDV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36097
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_018492.2 CDS:; g99a (silent); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 62907 Daltons