cDNA Clone ERBB3

• Synonyms: c-erbB3, c-erbB-3, ErbB-3, erbB3-S, HER3
• Plasmid: pGEX6P-2-ERBB3
• Parent Plasmid: pGEX6P2
• Expressed: GST-ERBB3
• Genbank: NM_001982.2 GI:54792099
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGGGCGAACGACGCTCTGCAGGTGCTGGGCTTGCTTTT
CAGCCTGGCCCGGGGCTCCGAGGTGGGCAACTCTCAGGCAGTGTGTCCTG
GGACTCTGAATGGCCTGAGTGTGACCGGCGATGCTGAGAACCAATACCAG
ACACTGTACAAGCTCTACGAGAGGTGTGAGGTGGTGATGGGGAACCTTGA
GATTGTGCTCACGGGACACAATGCCGACCTCTCCTTCCTGCAGTGGATTC
GAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCTACTCTA
CCATTGCCCAACCTCCGCGTGGTGCGAGGGACCCAGGTCTACGATGGGAA
GTTTGCCATCTTCGTCATGTTGAACTATAACACCAACTCCAGCCACGCTC
TGCGCCAGCTCCGCTTGACTCAGCTCACCGAGATTCTGTCAGGGGGTGTT
TATATTGAGAAGAACGATAAGCTTTGTCACATGGACACAATTGACTGGAG
GGACATCGTGAGGGACCGAGATGCTGAGATAGTGGTGAAGGACAATGGCA
GAAGCTGTCCCCCCTGTCATGAGGTTTGCAAGGGGCGATGCTGGGGTCCT
GGATCAGAAGACTGCCAGACATTGACCAAGACCATCTGTGCTCCTCAGTG
TAATGGTCACTGCTTTGGGCCCAACCCCAACCAGTGCTGCCATGATGAGT
GTGCCGGGGGCTGCTCAGGCCCTCAGGACACAGACTGCTTTGCCTGCCGG
CACTTCAATGACAGTGGAGCCTGTGTACCTCGCTGTCCACAGCCTCTTGT
CTACAACAAGCTAACTTTCCAGCTGGAACCCAATCCCCACACCAAGTATC
AGTATGGAGGAGTTTGTGTAGCCAGCTGTCCCCATAACTTTGTGGTGGAT
CAAACATCCTGTGTCAGGGCCTGTCCTCCTGACAAGATGGAAGTAGATAA
AAATGGGCTCAAGATGTGTGAGCCTTGTGGGGGACTATGTCCCAAAGCCT
GTGAGGGAACAGGCTCTGGGAGCCGCTTCCAGACTGTGGACTCGAGCAAC
ATTGATGGATTTGTGAACTGCACCAAGATCCTGGGCAACCTGGACTTTCT
GATCACCGGCCTCAATGGAGACCCCTGGCACAAGATCCCTGCCCTGGACC
CAGAGAAGCTCAATGTCTTCCGGACAGTACGGGAGATCACAGGTTACCTG
AACATCCAGTCCTGGCCGCCCCACATGCACAACTTCAGTGTTTTTTCCAA
TTTGACAACCATTGGAGGCAGAAGCCTCTACAACCGGGGCTTCTCATTGT
TGATCATGAAGAACTTGAATGTCACATCTCTGGGCTTCCGATCCCTGAAG
GAAATTAGTGCTGGGCGTATCTATATAAGTGCCAATAGGCAGCTCTGCTA
CCACCACTCTTTGAACTGGACCAAGGTGCTTCGGGGGCCTACGGAAGAGC
GACTAGACATCAAGCATAATCGGCCGCGCAGAGACTGCGTGGCAGAGGGC
AAAGTGTGTGACCCACTGTGCTCCTCTGGGGGATGCTGGGGCCCAGGCCC
TGGTCAGTGCTTGTCCTGTCGAAATTATAGCCGAGGAGGTGTCTGTGTGA
CCCACTGCAACTTTCTGAATGGGGAGCCTCGAGAATTTGCCCATGAGGCC
GAATGCTTCTCCTGCCACCCGGAATGCCAACCCATGGAGGGCACTGCCAC
ATGCAATGGCTCGGGCTCTGATACTTGTGCTCAATGTGCCCATTTTCGAG
ATGGGCCCCACTGTGTGAGCAGCTGCCCCCATGGAGTCCTAGGTGCCAAG
GGCCCAATCTACAAGTACCCAGATGTTCAGAATGAATGTCGGCCCTGCCA
TGAGAACTGCACCCAGGGGTGTAAAGGACCAGAGCTTCAAGACTGTTTAG
GACAAACACTGGTGCTGATCGGCAAAACCCATCTGACAATGGCTTTGACA
GTGATAGCAGGATTGGTAGTGATTTTCATGATGCTGGGCGGCACTTTTCT
CTACTGGCGTGGGCGCCGGATTCAGAATAAAAGGGCTATGAGGCGATACT
TGGAACGGGGTGAGAGCATAGAGCCTCTGGACCCCAGTGAGAAGGCTAAC
AAAGTCTTGGCCAGAATCTTCAAAGAGACAGAGCTAAGGAAGCTTAAAGT
GCTTGGCTCGGGTGTCTTTGGAACTGTGCACAAAGGAGTGTGGATCCCTG
AGGGTGAATCAATCAAGATTCCAGTCTGCATTAAAGTCATTGAGGACAAG
AGTGGACGGCAGAGTTTTCAAGCTGTGACAGATCATATGCTGGCCATTGG
CAGCCTGGACCATGCCCACATTGTAAGGCTGCTGGGACTATGCCCAGGGT
CATCTCTGCAGCTTGTCACTCAATATTTGCCTCTGGGTTCTCTGCTGGAT
CATGTGAGACAACACCGGGGGGCACTGGGGCCACAGCTGCTGCTCAACTG
GGGAGTACAAATTGCCAAGGGAATGTACTACCTTGAGGAACATGGTATGG
TGCATAGAAACCTGGCTGCCCGAAACGTGCTACTCAAGTCACCCAGTCAG
GTTCAGGTGGCAGATTTTGGTGTGGCTGACCTGCTGCCTCCTGATGATAA
GCAGCTGCTATACAGTGAGGCCAAGACTCCAATTAAGTGGATGGCCCTTG
AGAGTATCCACTTTGGGAAATACACACACCAGAGTGATGTCTGGAGCTAT
GGTGTGACAGTTTGGGAGTTGATGACCTTCGGGGCAGAGCCCTATGCAGG
GCTACGATTGGCTGAAGTACCAGACCTGCTAGAGAAGGGGGAGCGGTTGG
CACAGCCCCAGATCTGCACAATTGATGTCTACATGGTGATGGTCAAGTGT
TGGATGATTGATGAGAACATTCGCCCAACCTTTAAAGAACTAGCCAATGA
GTTCACCAGGATGGCCCGAGACCCACCACGGTATCTGGTCATAAAGAGAG
AGAGTGGGCCTGGAATAGCCCCTGGGCCAGAGCCCCATGGTCTGACAAAC
AAGAAGCTAGAGGAAGTAGAGCTGGAGCCAGAACTAGACCTAGACCTAGA
CTTGGAAGCAGAGGAGGACAACCTGGCAACCACCACACTGGGCTCCGCCC
TCAGCCTACCAGTTGGAACACTTAATCGGCCACGTGGGAGCCAGAGCCTT
TTAAGTCCATCATCTGGATACATGCCCATGAACCAGGGTAATCTTGGGGA
GTCTTGCCAGGAGTCTGCAGTTTCTGGGAGCAGTGAACGGTGCCCCCGTC
CAGTCTCTCTACACCCAATGCCACGGGGATGCCTGGCATCAGAGTCATCA
GAGGGGCATGTAACAGGCTCTGAGGCTGAGCTCCAGGAGAAAGTGTCAAT
GTGTAGGAGCCGGAGCAGGAGCCGGAGCCCACGGCCACGCGGAGATAGCG
CCTACCATTCCCAGCGCCACAGTCTGCTGACTCCTGTTACCCCACTCTCC
CCACCCGGGTTAGAGGAAGAGGATGTCAACGGTTATGTCATGCCAGATAC
ACACCTCAAAGGTACTCCCTCCTCCCGGGAAGGCACCCTTTCTTCAGTGG
GTCTCAGTTCTGTCCTGGGTACTGAAGAAGAAGATGAAGATGAGGAGTAT
GAATACATGAACCGGAGGAGAAGGCACAGTCCACCTCATCCCCCTAGGCC
AAGTTCCCTTGAGGAGCTGGGTTATGAGTACATGGATGTGGGGTCAGACC
TCAGTGCCTCTCTGGGCAGCACACAGAGTTGCCCACTCCACCCTGTACCC
ATCATGCCCACTGCAGGCACAACTCCAGATGAAGACTATGAATATATGAA
TCGGCAACGAGATGGAGGTGGTCCTGGGGGTGATTATGCAGCCATGGGGG
CCTGCCCAGCATCTGAGCAAGGGTATGAAGAGATGAGAGCTTTTCAGGGG
CCTGGACATCAGGCCCCCCATGTCCATTATGCCCGCCTAAAAACTCTACG
TAGCTTAGAGGCTACAGACTCTGCCTTTGATAACCCTGATTACTGGCATA
GCAGGCTTTTCCCCAAGGCTAATGCCCAGAGAACGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMRANDALQ
VLGLLFSLARGSEVGNSQAVCPGTLNGLSVTGDAENQYQTLYKLYERCEV
VMGNLEIVLTGHNADLSFLQWIREVTGYVLVAMNEFSTLPLPNLRVVRGT
QVYDGKFAIFVMLNYNTNSSHALRQLRLTQLTEILSGGVYIEKNDKLCHM
DTIDWRDIVRDRDAEIVVKDNGRSCPPCHEVCKGRCWGPGSEDCQTLTKT
ICAPQCNGHCFGPNPNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPR
CPQPLVYNKLTFQLEPNPHTKYQYGGVCVASCPHNFVVDQTSCVRACPPD
KMEVDKNGLKMCEPCGGLCPKACEGTGSGSRFQTVDSSNIDGFVNCTKIL
GNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHN
FSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRIYISA
NRQLCYHHSLNWTKVLRGPTEERLDIKHNRPRRDCVAEGKVCDPLCSSGG
CWGPGPGQCLSCRNYSRGGVCVTHCNFLNGEPREFAHEAECFSCHPECQP
MEGTATCNGSGSDTCAQCAHFRDGPHCVSSCPHGVLGAKGPIYKYPDVQN
ECRPCHENCTQGCKGPELQDCLGQTLVLIGKTHLTMALTVIAGLVVIFMM
LGGTFLYWRGRRIQNKRAMRRYLERGESIEPLDPSEKANKVLARIFKETE
LRKLKVLGSGVFGTVHKGVWIPEGESIKIPVCIKVIEDKSGRQSFQAVTD
HMLAIGSLDHAHIVRLLGLCPGSSLQLVTQYLPLGSLLDHVRQHRGALGP
QLLLNWGVQIAKGMYYLEEHGMVHRNLAARNVLLKSPSQVQVADFGVADL
LPPDDKQLLYSEAKTPIKWMALESIHFGKYTHQSDVWSYGVTVWELMTFG
AEPYAGLRLAEVPDLLEKGERLAQPQICTIDVYMVMVKCWMIDENIRPTF
KELANEFTRMARDPPRYLVIKRESGPGIAPGPEPHGLTNKKLEEVELEPE
LDLDLDLEAEEDNLATTTLGSALSLPVGTLNRPRGSQSLLSPSSGYMPMN
QGNLGESCQESAVSGSSERCPRPVSLHPMPRGCLASESSEGHVTGSEAEL
QEKVSMCRSRSRSRSPRPRGDSAYHSQRHSLLTPVTPLSPPGLEEEDVNG
YVMPDTHLKGTPSSREGTLSSVGLSSVLGTEEEDEDEEYEYMNRRRRHSP
PHPPRPSSLEELGYEYMDVGSDLSASLGSTQSCPLHPVPIMPTAGTTPDE
DYEYMNRQRDGGGPGGDYAAMGACPASEQGYEEMRAFQGPGHQAPHVHYA
RLKTLRSLEATDSAFDNPDYWHSRLFPKANAQRT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36094
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 175897 Daltons