cDNA Clone RSK2

• Synonyms: RPS6KA3, MAPKAP-K1b, CLS, HU-3, MRX19, p90-RSK2, pp90RSK2, RSK, RSK2, S6K-alpha3
• Plasmid: pEBG6P-1 RSK2
• Parent Plasmid: pEBG6P
• Expressed: GST-RSK2
• Genbank: NM_004586.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCGCTGGCGCAGCTGGCGGACCCGTGGCAGAAGATGGCTGT
GGAGAGCCCGTCCGACAGCGCTGAGAATGGACAGCAAATTATGGATGAAC
CTATGGGAGAGGAGGAGATTAACCCACAAACTGAAGAAGTCAGTATCAAA
GAAATTGCAATCACACATCATGTAAAGGAAGGACATGAAAAGGCAGATCC
TTCCCAGTTTGAACTTTTAAAAGTATTAGGGCAGGGATCATTTGGAAAGG
TTTTCTTAGTTAAAAAAATCTCAGGCTCTGATGCTAGGCAGCTTTATGCC
ATGAAGGTATTGAAGAAGGCCACACTGAAAGTTCGAGACCGAGTTCGGAC
AAAAATGGAACGTGATATCTTGGTAGAGGTTAATCATCCTTTTATTGTCA
AGTTGCATTATGCTTTTCAAACTGAAGGGAAGTTGTATCTTATTTTGGAT
TTTCTCAGGGGAGGAGATTTGTTTACACGCTTATCCAAAGAGGTGATGTT
CACAGAAGAAGATGTCAAATTCTACTTGGCTGAACTTGCACTTGCTTTAG
ACCATCTACATAGCCTGGGAATAATTTATAGAGACTTAAAACCAGAAAAT
ATACTTCTTGATGAAGAAGGTCACATCAAGTTAACAGATTTCGGCCTAAG
TAAAGAGTCTATTGACCATGAAAAGAAGGCATATTCTTTTTGTGGAACTG
TGGAGTATATGGCTCCAGAAGTAGTTAATCGTCGAGGTCATACTCAGAGT
GCTGACTGGTGGTCTTTTGGTGTGTTAATGTTTGAAATGCTTACTGGTAC
ACTACCTTTCCAAGGAAAAGATCGAAAAGAAACAATGACTATGATTCTTA
AAGCCAAACTTGGAATGCCACAGTTTTTGAGTCCTGAAGCGCAGAGTCTT
TTACGAATGCTTTTCAAGCGAAATCCTGCAAACAGATTAGGTGCAGGACC
AGATGGAGTTGAAGAAATTAAAAGACATTCATTTTTCTCAACGATAGACT
GGAATAAACTGTATAGAAGAGAAATTCATCCGCCATTTAAACCTGCAACG
GGCAGGCCTGAAGATACATTCTATTTTGATCCTGAGTTTACTGCAAAAAC
TCCCAAAGATTCACCTGGCATTCCACCTAGTGCTAATGCACATCAGCTTT
TTCGGGGGTTTAGTTTTGTTGCTATTACCTCAGATGATGAAAGCCAAGCT
ATGCAGACAGTTGGTGTACATTCAATTGTTCAGCAGTTACACAGGAACAG
TATTCAGTTTACTGATGGATATGAAGTAAAAGAAGATATTGGAGTTGGCT
CCTACTCTGTTTGCAAGAGATGTATACATAAAGCTACAAACATGGAGTTT
GCAGTGAAGATTATTGATAAAAGCAAGAGAGACCCAACAGAAGAAATTGA
AATTCTTCTTCGTTATGGACAGCATCCAAACATTATCACTCTAAAGGATG
TATATGATGATGGAAAGTATGTGTATGTAGTAACAGAACTTATGAAAGGA
GGTGAATTGCTGGATAAAATTCTTAGACAAAAATTTTTCTCTGAACGAGA
GGCCAGTGCTGTCCTGTTCACTATAACTAAAACCGTTGAATATCTTCACG
CACAAGGGGTGGTTCATAGAGACTTGAAACCTAGCAACATTCTTTATGTG
GATGAATCTGGTAATCCGGAATCTATTCGAATTTGTGATTTTGGCTTTGC
AAAACAGCTGAGAGCGGAAAATGGTCTTCTCATGACTCCTTGTTACACTG
CAAATTTTGTTGCACCAGAGGTTTTAAAAAGACAAGGCTATGATGCTGCT
TGTGATATATGGAGTCTTGGTGTCCTACTCTATACAATGCTTACCGGTTA
CACTCCATTTGCAAATGGTCCTGATGATACACCAGAGGAAATATTGGCAC
GAATAGGTAGCGGAAAATTCTCACTCAGTGGTGGTTACTGGAATTCTGTT
TCAGACACAGCAAAGGACCTGGTGTCAAAGATGCTTCATGTAGACCCTCA
TCAGAGACTGACTGCTGCTCTTGTGCTCAGACATCCTTGGATCGTCCACT
GGGACCAACTGCCACAATACCAACTAAACAGACAGGATGCACCACATCTA
GTAAAGGGTGCCATGGCAGCTACATATTCTGCTTTGAACCGTAATCAGTC
ACCAGTTTTGGAACCAGTAGGCCGCTCTACTCTTGCTCAGCGGAGAGGTA
TTAAAAAAATCACCTCAACAGCCCTGTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPLAQLADPWQKM
AVESPSDSAENGQQIMDEPMGEEEINPQTEEVSIKEIAITHHVKEGHEKA
DPSQFELLKVLGQGSFGKVFLVKKISGSDARQLYAMKVLKKATLKVRDRV
RTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEV
MFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKLTDFG
LSKESIDHEKKAYSFCGTVEYMAPEVVNRRGHTQSADWWSFGVLMFEMLT
GTLPFQGKDRKETMTMILKAKLGMPQFLSPEAQSLLRMLFKRNPANRLGA
GPDGVEEIKRHSFFSTIDWNKLYRREIHPPFKPATGRPEDTFYFDPEFTA
KTPKDSPGIPPSANAHQLFRGFSFVAITSDDESQAMQTVGVHSIVQQLHR
NSIQFTDGYEVKEDIGVGSYSVCKRCIHKATNMEFAVKIIDKSKRDPTEE
IEILLRYGQHPNIITLKDVYDDGKYVYVVTELMKGGELLDKILRQKFFSE
REASAVLFTITKTVEYLHAQGVVHRDLKPSNILYVDESGNPESIRICDFG
FAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGVLLYTMLT
GYTPFANGPDDTPEEILARIGSGKFSLSGGYWNSVSDTAKDLVSKMLHVD
PHQRLTAALVLRHPWIVHWDQLPQYQLNRQDAPHLVKGAMAATYSALNRN
QSPVLEPVGRSTLAQRRGIKKITSTAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36055
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express RSK2 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 111150 Daltons