cDNA Clone TESK1

• Plasmid: pEBG6P-1 TESK1
• Parent Plasmid: pEBG6P
• Expressed: GST-TESK1
• Genbank: NM_006285.2 GI:66932998*
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGGCCGGGGAACGGCCCCCACTGCGGGGCCCTGGGCCCGGGCC
TGGAGAGGTGCCGGGGGAGGGGCCCCCGGGGCCGGGGGGCACGGGCGGAG
GCCCGGGCCGGGGCCGCCCCTCCTCCTACCGGGCTCTCCGCAGCGCCGTG
TCTAGCCTGGCGCGTGTGGACGATTTTCACTGCGCGGAGAAGATCGGGGC
CGGCTTCTTCTCTGAGGTCTACAAGGTTCGGCACCGACAGTCAGGGCAAG
TCATGGTGCTGAAGATGAACAAGCTCCCCAGTAACCGGGGCAACACACTA
CGGGAAGTGCAGCTGATGAACCGGCTCAGGCACCCCAACATCCTAAGGTT
CATGGGAGTCTGTGTGCACCAGGGACAGCTGCACGCTCTTACAGAGTATA
TGAATGGGGGGACATTGGAACAGCTGCTCAGCTCCCCTGAACCCCTATCC
TGGCCGGTCAGGCTCCACCTGGCCCTGGACATTGCCCGAGGCCTGCGGTA
CCTGCACTCCAAAGGTGTATTTCACCGCGACCTCACATCCAAGAACTGTC
TAGTCCGACGGGAAGATCGAGGCTTCACCGCTGTCGTGGGTGACTTCGGG
CTGGCCGAAAAGATTCCTGTGTATAGGGAGGGGGCAAGGAAGGAGCCATT
GGCCGTGGTGGGCTCCCCATACTGGATGGCTCCAGAGGTGTTACGGGGTG
AGCTGTATGATGAGAAGGCTGATGTCTTTGCCTTCGGGATTGTCCTCTGT
GAGCTCATCGCCCGAGTACCTGCAGACCCTGACTACCTACCACGCACTGA
GGACTTTGGCCTGGATGTGCCTGCTTTCCGAACTCTGGTGGGGGATGACT
GCCCACTGCCTTTCTTGCTCCTGGCCATCCACTGCTGCAACCTGGAACCC
AGCACCCGTGCCCCCTTCACCGAAATTACCCAGCACCTGGAATGGATCCT
GGAGCAGCTGCCTGAGCCAGCCCCACTCACCAGGACCGCCCTGACACACA
ATCAGGGCTCTGTTGCAAGAGGGGGTCCCTCTGCCACGCTTCCCAGGCCA
GATCCCCGGCTTTCCCGAAGCCGGTCAGACCTCTTCCTGCCCCCATCACC
AGAATCACCCCCCAACTGGGGGGACAATCTGACTCGAGTCAACCCCTTCT
CACTACGGGAAGACCTCAGGGGTGGCAAGATCAAGCTCTTAGACACACCC
AGCAAGCCAGTCCTGCCTCTTGTGCCACCATCACCATTCCCATCCACTCA
GCTGCCCTTGGTGACCACTCCGGAGACCCTGGTCCAGCCTGGGACACCTG
CCCGCCGCTGCCGCTCACTACCCTCATCCCCCGAGCTCCCCCGCCGTATG
GAGACAGCACTGCCAGGTCCTGGCCCTCCCGCTGTGGGCCCCTCGGCTGA
AGAGAAGATGGAGTGCGAGGGCAGCAGCCCTGAGCCGGAACCTCCAGGGC
CAGCGCCCCAGCTGCCTCTGGCTGTGGCCACAGACAACTTCATCAGCACC
TGTTCCTCGGCCTCCCAACCCTGGTCCCCTAGATCAGGACCCGTCCTCAA
TAACAATCCCCCAGCTGTGGTGGTGAACTCCCCACAAGGCTGGGCTGGGG
AGCCCTGGAACCGGGCCCAGCATAGCCTGCCCCGGGCGGCAGCCCTGGAG
CGGACAGAACCCTCGCCACCCCCTTCAGCTCCCCGGGAGCCCGATGAGGG
GCTGCCCTGTCCTGGCTGCTGCCTCGGCCCCTTCAGCTTTGGCTTCCTGT
CCATGTGCCCCCGCCCCACACCAGCTGTTGCCCGCTACCGCAACCTGAAC
TGTGAGGCGGGCAGTCTCCTCTGCCACCGAGGGCACCACGCCAAGCCACC
CACACCCAGCCTGCAGCTGCCTGGGGCACGCTCTTAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAGERPPLRGPGP
GPGEVPGEGPPGPGGTGGGPGRGRPSSYRALRSAVSSLARVDDFHCAEKI
GAGFFSEVYKVRHRQSGQVMVLKMNKLPSNRGNTLREVQLMNRLRHPNIL
RFMGVCVHQGQLHALTEYMNGGTLEQLLSSPEPLSWPVRLHLALDIARGL
RYLHSKGVFHRDLTSKNCLVRREDRGFTAVVGDFGLAEKIPVYREGARKE
PLAVVGSPYWMAPEVLRGELYDEKADVFAFGIVLCELIARVPADPDYLPR
TEDFGLDVPAFRTLVGDDCPLPFLLLAIHCCNLEPSTRAPFTEITQHLEW
ILEQLPEPAPLTRTALTHNQGSVARGGPSATLPRPDPRLSRSRSDLFLPP
SPESPPNWGDNLTRVNPFSLREDLRGGKIKLLDTPSKPVLPLVPPSPFPS
TQLPLVTTPETLVQPGTPARRCRSLPSSPELPRRMETALPGPGPPAVGPS
AEEKMECEGSSPEPEPPGPAPQLPLAVATDNFISTCSSASQPWSPRSGPV
LNNNPPAVVVNSPQGWAGEPWNRAQHSLPRAAALERTEPSPPPSAPREPD
EGLPCPGCCLGPFSFGFLSMCPRPTPAVARYRNLNCEAGSLLCHRGHHAK
PPTPSLQLPGARS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36042
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_006285.2 CDS:; g441a (silent); Mammalian expression plasmid to express STAT3 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 95099 Daltons