cDNA Clone SCRIB

• Plasmid: pCMV5-HA SCRIB
• Parent Plasmid: pCMV5 HA1
• Expressed: HA SCRIB
• Genbank: NM_182706.3
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCTCAAGTGCATCCCGCTGTGGCGCTGCAACCGGCACGTGGA
GTCGGTGGACAAGCGGCACTGTTCGCTGCAGGCCGTGCCGGAGGAGATCT
ACCGCTACAGCCGCAGCCTGGAGGAGCTGCTGCTCGACGCCAACCAGCTG
CGCGAGCTGCCCAAGCCTTTTTTCCGGCTGCTGAACTTGCGCAAGCTGGG
CCTGAGCGACAACGAGATCCAGCGGTTGCCTCCCGAGGTGGCCAACTTCA
TGCAGCTGGTGGAGCTGGACGTGTCCCGGAACGATATCCCTGAGATCCCG
GAGAGCATCAAGTTCTGCAAGGCTCTGGAGATCGCGGACTTCAGCGGGAA
CCCCCTCTCCAGGCTCCCTGATGGCTTCACTCAGCTGCGCAGCCTGGCTC
ACCTGGCCCTGAATGATGTGTCTCTGCAGGCACTGCCCGGGGACGTGGGC
AACCTCGCCAACCTGGTGACCCTGGAGCTCCGGGAGAACCTGCTCAAGTC
CCTGCCAGCGTCCCTGTCATTTCTGGTCAAGCTGGAACAGCTGGATCTGG
GAGGCAACGATCTGGAAGTGCTGCCAGACACTCTGGGGGCTCTGCCCAAT
CTTCGGGAGCTGTGGCTTGACCGGAACCAGCTGTCAGCACTGCCCCCGGA
GCTCGGGAACCTGCGGCGCCTGGTGTGCCTGGACGTGTCGGAAAACCGGC
TGGAGGAGCTGCCTGCTGAGCTCGGCGGGCTGGTGCTGCTCACTGACCTG
CTGCTGTCCCAGAACCTGCTGCGGAGGCTGCCCGACGGCATCGGTCAGCT
GAAGCAGCTATCCATCCTAAAGGTAGACCAGAATCGGCTGTGCGAGGTGA
CTGAGGCCATCGGGGACTGTGAGAACCTCTCTGAGCTGATCCTCACGGAG
AACCTGCTGATGGCCCTGCCCCGCTCCCTGGGAAAGCTGACTAAGCTGAC
CAACCTCAACGTGGACCGGAACCACCTCGAGGCGCTGCCGCCCGAGATCG
GGGGCTGTGTGGCACTCAGCGTCCTCTCCTTGAGGGACAACCGCCTGGCC
GTCCTGCCACCAGAGCTGGCCCACACGACAGAGCTGCACGTGCTGGACGT
GGCGGGGAACCGCCTGCAGAGTCTGCCGTTCGCGCTCACCCACCTCAATC
TCAAGGCCCTGTGGCTGGCAGAGAACCAGGCGCAGCCCATGCTCCGGTTC
CAGACGGAGGATGATGCCCGGACCGGCGAGAAGGTGCTCACCTGCTACTT
GCTGCCCCAGCAGCCCCCACTCAGCCTCGAGGATGCTGGGCAGCAGGGGA
GCCTCTCGGAGACCTGGAGCGATGCCCCGCCGAGCCGCGTCAGCGTCATC
CAGTTCCTGGAGGCCCCCATAGGTGATGAGGACGCTGAGGAAGCTGCAGC
TGAGAAGCGGGGCCTACAGCGCCGGGCCACACCTCACCCCAGCGAGCTCA
AGGTGATGAAGAGGAGCATCGAGGGGCGGCGGAGCGAGGCCTGCCCTTGC
CAGCCAGACTCTGGGTCGCCCTTGCCTGCAGAGGAGGAGAAGCGGCTGAG
TGCCGAGTCTGGCCTGAGTGAAGACTCTCGCCCATCTGCCAGCACAGTCT
CTGAGGCTGAGCCCGAGGGCCCGTCGGCTGAGGCACAGGGTGGGAGCCAG
CAGGAAGCCACGACTGCTGGCGGGGAGGAAGACGCCGAAGAGGACTACCA
GGAGCCCACGGTGCATTTCGCAGAGGACGCACTGCTGCCCGGGGATGACA
GGGAGATCGAGGAGGGGCAGCCTGAGGCCCCCTGGACCCTGCCAGGCGGG
AGGCAGCGGCTCATCCGCAAGGACACACCTCACTACAAAAAGCACTTCAA
GATCTCCAAGCTGCCCCAGCCCGAGGCCGTTGTGGCTCTGCTGCAGGGCA
TGCAGCCTGATGGGGAGGGCCCTGTGGCTCCCGGGGGCTGGCACAATGGC
CCCCACGCACCCTGGGCTCCTCGGGCCCAGAAGGAGGAGGAGGAGGAGGA
AGAGGGTAGTCCTCAGGAGGAGGAGGAAGAGGAGGAGGAGGAAAACAGGG
CTGAAGAGGAAGAGGCCAGCACTGAGGAGGAGGACAAGGAGGGGGCCGTG
GTTTCTGCGCCCTCTGTCAAGGGAGTGTCGTTTGACCAGGCCAATAACCT
GCTGATAGAGCCTGCTCGCATTGAGGAGGAAGAGCTGACCCTCACCATCC
TGCGGCAGACTGGGGGCCTGGGCATCAGCATTGCGGGCGGCAAGGGCTCC
ACACCCTATAAGGGGGACGACGAGGGCATATTCATCTCTCGGGTGTCCGA
GGAAGGCCCTGCGGCCCGGGCTGGAGTCCGTGTGGGTGACAAGCTCCTGG
AGGTGAATGGTGTGGCTCTGCAGGGCGCCGAGCACCACGAGGCCGTGGAG
GCGCTCCGGGGGGCCGGCACTGCCGTGCAGATGCGAGTGTGGCGGGAGCG
CATGGTGGAGCCTGAGAACGCGGTCACCATCACGCCGCTGCGGCCCGAGG
ATGATTACAGCCCCCGAGAGCGGCGGGGAGGGGGGCTGCGCCTGCCCCTG
CTCCCGCCTGAGAGCCCCGGGCCCCTCCGTCAGCGCCACGTGGCCTGCCT
GGCACGCAGCGAGAGGGGGCTGGGCTTCAGCATTGCTGGTGGGAAAGGCT
CCACACCCTACAGGGCTGGTGATGCGGGCATCTTCGTCTCCCGCATTGCC
GAGGGCGGTGCTGCTCACCGCGCGGGCACACTGCAGGTTGGCGACCGCGT
CCTCTCTATTAATGGAGTGGACGTGACTGAGGCCAGGCATGACCACGCCG
TCTCCCTGCTGACCGCTGCCTCCCCCACCATCGCCCTGCTGTTGGAGCGG
GAGGCTGGGGGCCCTCTTCCTCCCAGCCCTCTGCCACATTCCTCACCCCC
CACCGCTGCTGTTGCCACCACCAGCATAACCACTGCCACCCCCGGGGTGC
CTGGGTTGCCGAGCCTGGCCCCCAGCCTGCTGGCTGCCGCGTTGGAAGGG
CCATACCCAGTGGAGGAGATCCGTCTGCCAAGAGCTGGGGGCCCTCTGGG
GCTTAGTATTGTCGGAGGCTCCGACCATTCCAGCCACCCGTTTGGTGTCC
AGGAGCCTGGTGTGTTCATCTCCAAGGTGCTCCCGCGGGGCCTGGCCGCT
CGCAGCGGCCTGCGGGTTGGGGACCGCATCCTGGCAGTGAACGGGCAAGA
CGTGCGGGATGCCACGCACCAAGAAGCAGTCAGTGCCCTGCTCCGGCCCT
GCCTGGAGCTGTCGCTGCTGGTGCGGAGGGACCCGGCACCCCCGGGCCTA
CGGGAACTGTGCATCCAGAAGGCACCTGGGGAGAGGCTGGGCATCAGCAT
CCGCGGGGGTGCCAGGGGCCACGCTGGCAACCCCCGCGACCCCACAGACG
AGGGCATCTTCATCTCCAAGGTGAGCCCCACGGGGGCAGCCGGGCGCGAC
GGTCGGCTGCGTGTGGGTTTGCGGCTGTTGGAGGTGAACCAGCAGAGCCT
GCTGGGCCTGACGCACGGCGAGGCGGTGCAGCTGCTCCGCAGTGTGGGCG
ACACCCTCACCGTGCTGGTCTGTGACGGCTTCGAGGCCAGCACCGACGCA
GCCCTGGAGGTGTCCCCAGGTGTCATTGCCAACCCCTTTGCGGCAGGCAT
CGGCCACCGGAACAGCCTGGAGAGCATCTCTTCCATCGACCGGGAGCTGA
GCCCTGAGGGCCCAGGCAAGGAGAAGGAGCTGCCTGGACAGACCCTGCAC
TGGGGGCCCGAGGCCACAGAAGCCGCAGGTCGGGGTCTGCAGCCCCTGAA
GCTGGACTACCGCGCCCTGGCCGCCGTGCCCAGCGCTGGCAGCGTGCAGA
GGGTACCGTCTGGAGCAGCTGGAGGGAAGATGGCTGAATCTCCCTGCTCC
CCTAGTGGCCAGCAGCCGCCCTCCCCGCCTTCTCCGGATGAGCTGCCCGC
CAATGTGAAGCAGGCCTACAGGGCCTTCGCGGCCGTGCCCACTTCTCACC
CGCCTGAGGATGCCCCTGCCCAGCCCCCCACGCCTGGGCCTGCAGCCTCC
CCGGAGCAGCTGTCCTTCCGGGAGCGGCAGAAGTACTTTGAGCTGGAGGT
GCGCGTGCCCCAGGCCGAGGGCCCCCCTAAGCGCGTGTCCCTGGTGGGTG
CTGACGACCTGCGGAAGATGCAGGAGGAGGAAGCCAGAAAACTACAGCAG
AAGAGAGCGCAGATGCTGCGGGAGGCGGCAGAGGCTGGGGCCGAAGCGAG
GCTCGCCCTGGACGGGGAGACGCTGGGCGAGGAGGAACAGGAGGATGAGC
AGCCACCCTGGGCCAGCCCGAGCCCCACCTCAAGGCAGAGCCCGGCGTCC
CCCCCGCCCCTGGGAGGTGGCGCCCCGGTGCGGACGGCCAAAGCTGAACG
GCGCCACCAGGAGCGGCTGCGCGTGCAGAGTCCGGAGCCACCGGCACCCG
AGCGTGCCCTGTCCCCTGCCGAGCTCCGGGCCCTGGAGGCCGAGAAGCGT
GCGCTGTGGAGGGCAGCCAGGATGAAGTCATTGGAACAGGACGCTCTCCG
AGCACAGATGGTCCTCAGCAGGTCCCAGGAAGGCCGGGGCACGCGGGGGC
CCCTGGAGCGACTGGCCGAGGCCCCTTCCCCTGCGCCCACCCCGTCGCCC
ACCCCTGTGGAAGACCTCGGCCCCCAGACCAGCACCTCCCCGGGACGCCT
GCCCTTGTCTGGAAAGAAGTTTGACTACAGGGCCTTTGCGGCCCTGCCTT
CTTCCAGACCTGTCTATGACATCCAGTCACCGGACTTTGCTGAGGAGTTG
AGGTCCCTGGAACCATCTCCCAGCCCTGGCCCGCAGGAGGAGGATGGAGA
AGTGGCTCTGGTGCTTCTGGGCAGGCCCTCACCCGGCGCTGTGGGCCCTG
AAGATGTGGCACTGTGCAGCAGCCGCCGCCCCGTAAGGCCTGGGCGCCGT
GGCCTGGGCCCTGTGCCCTCCTAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYRYSRSL
EELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELD
VSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDV
SLQALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEV
LPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPAE
LGGLVLLTDLLLSQNLLRRLPDGIGQLKQLSILKVDQNRLCEVTEAIGDC
ENLSELILTENLLMALPRSLGKLTKLTNLNVDRNHLEALPPEIGGCVALS
VLSLRDNRLAVLPPELAHTTELHVLDVAGNRLQSLPFALTHLNLKALWLA
ENQAQPMLRFQTEDDARTGEKVLTCYLLPQQPPLSLEDAGQQGSLSETWS
DAPPSRVSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSELKVMKRSI
EGRRSEACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSEAEPEG
PSAEAQGGSQQEATTAGGEEDAEEDYQEPTVHFAEDALLPGDDREIEEGQ
PEAPWTLPGGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQGMQPDGEG
PVAPGGWHNGPHAPWAPRAQKEEEEEEEGSPQEEEEEEEEENRAEEEEAS
TEEEDKEGAVVSAPSVKGVSFDQANNLLIEPARIEEEELTLTILRQTGGL
GISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVAL
QGAEHHEAVEALRGAGTAVQMRVWRERMVEPENAVTITPLRPEDDYSPRE
RRGGGLRLPLLPPESPGPLRQRHVACLARSERGLGFSIAGGKGSTPYRAG
DAGIFVSRIAEGGAAHRAGTLQVGDRVLSINGVDVTEARHDHAVSLLTAA
SPTIALLLEREAGGPLPPSPLPHSSPPTAAVATTSITTATPGVPGLPSLA
PSLLAAALEGPYPVEEIRLPRAGGPLGLSIVGGSDHSSHPFGVQEPGVFI
SKVLPRGLAARSGLRVGDRILAVNGQDVRDATHQEAVSALLRPCLELSLL
VRRDPAPPGLRELCIQKAPGERLGISIRGGARGHAGNPRDPTDEGIFISK
VSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLV
CDGFEASTDAALEVSPGVIANPFAAGIGHRNSLESISSIDRELSPEGPGK
EKELPGQTLHWGPEATEAAGRGLQPLKLDYRALAAVPSAGSVQRVPSGAA
GGKMAESPCSPSGQQPPSPPSPDELPANVKQAYRAFAAVPTSHPPEDAPA
QPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLVGADDLRKM
QEEEARKLQQKRAQMLREAAEAGAEARLALDGETLGEEEQEDEQPPWASP
SPTSRQSPASPPPLGGGAPVRTAKAERRHQERLRVQSPEPPAPERALSPA
ELRALEAEKRALWRAARMKSLEQDALRAQMVLSRSQEGRGTRGPLERLAE
APSPAPTPSPTPVEDLGPQTSTSPGRLPLSGKKFDYRAFAALPSSRPVYD
IQSPDFAEELRSLEPSPSPGPQEEDGEVALVLLGRPSPGAVGPEDVALCS
SRRPVRPGRRGLGPVPS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31900
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 179090 Daltons