cDNA Clone ATP1A1

• Plasmid: pcDNA5-FRT/TO ATP1A1-FLAG
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: ATP1A1-FLAG
• Genbank: NM_000701.6 GI:48762680 (with silent change to kill internal BamH1 site)
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGGGAAGGGGGTTGGACGTGATAAGTATGAGCCTGC
AGCTGTTTCAGAACAAGGTGATAAAAAGGGCAAAAAGGGCAAAAAAGACA
GGGACATGGATGAACTGAAGAAAGAAGTTTCTATGGATGATCATAAACTT
AGCCTTGATGAACTTCATCGTAAATATGGAACAGACTTGAGCCGGGGATT
AACATCTGCTCGTGCAGCTGAGATCCTGGCGCGAGATGGTCCCAACGCCC
TCACTCCCCCTCCCACTACTCCTGAATGGATCAAGTTTTGTCGGCAGCTC
TTTGGGGGGTTCTCAATGTTACTGTGGATTGGAGCGATTCTTTGTTTCTT
GGCTTATAGCATCCAAGCTGCTACAGAAGAGGAACCTCAAAACGATAATC
TGTACCTGGGTGTGGTGCTATCAGCCGTTGTAATCATAACTGGTTGCTTC
TCCTACTATCAAGAAGCTAAAAGTTCAAAGATCATGGAATCCTTCAAAAA
CATGGTCCCTCAGCAAGCCCTTGTGATTCGAAATGGTGAGAAAATGAGCA
TAAATGCGGAGGAAGTTGTGGTTGGGGATCTGGTGGAAGTAAAAGGAGGA
GACCGAATTCCTGCTGACCTCAGAATCATATCTGCAAATGGCTGCAAGGT
GGATAACTCCTCGCTCACTGGTGAATCAGAACCCCAGACTAGGTCTCCAG
ATTTCACAAATGAAAACCCCCTGGAGACGAGGAACATTGCCTTCTTTTCA
ACCAATTGTGTTGAAGGCACCGCACGTGGTATTGTTGTCTACACTGGGGA
TCGCACTGTGATGGGAAGAATTGCCACACTTGCTTCTGGGCTGGAAGGAG
GCCAGACCCCCATTGCTGCAGAAATTGAACATTTTATCCACATCATCACG
GGTGTGGCTGTGTTCCTGGGTGTGTCTTTCTTCATCCTTTCTCTCATCCT
TGAGTACACCTGGCTTGAGGCTGTCATCTTCCTCATCGGTATCATCGTAG
CCAATGTGCCGGAAGGTTTGCTGGCCACTGTCACGGTCTGTCTGACACTT
ACTGCCAAACGCATGGCAAGGAAAAACTGCTTAGTGAAGAACTTAGAAGC
TGTGGAGACCTTGGGGTCCACGTCCACCATCTGCTCTGATAAAACTGGAA
CTCTGACTCAGAACCGGATGACAGTGGCCCACATGTGGTTTGACAATCAA
ATCCATGAAGCTGATACGACAGAGAATCAGAGTGGTGTCTCTTTTGACAA
GACTTCAGCTACCTGGCTTGCTCTGTCCAGAATTGCAGGTCTTTGTAACA
GGGCAGTGTTTCAGGCTAACCAGGAAAACCTACCTATTCTTAAGCGGGCA
GTTGCAGGAGATGCCTCTGAGTCAGCACTCTTAAAGTGCATAGAGCTGTG
CTGTGGTTCCGTGAAGGAGATGAGAGAAAGATACGCCAAAATCGTCGAGA
TACCCTTCAACTCCACCAACAAGTACCAGTTGTCTATTCATAAGAACCCC
AACACATCGGAGCCCCAACACCTGTTGGTGATGAAGGGCGCCCCAGAAAG
AATCCTAGACCGTTGCAGCTCTATCCTCCTCCACGGCAAGGAGCAGCCCC
TGGATGAGGAGCTGAAAGACGCCTTTCAGAACGCCTATTTGGAGCTGGGG
GGCCTCGGAGAACGAGTCCTAGGTTTCTGCCACCTCTTTCTGCCAGATGA
ACAGTTTCCTGAAGGGTTCCAGTTTGACACTGACGATGTGAATTTCCCTA
TCGATAATCTGTGCTTTGTTGGGCTCATCTCCATGATTGACCCTCCACGG
GCGGCCGTTCCTGATGCCGTGGGCAAATGTCGAAGTGCTGGAATTAAGGT
CATCATGGTCACAGGAGACCATCCAATCACAGCTAAAGCTATTGCCAAAG
GTGTGGGCATCATCTCAGAAGGCAATGAGACCGTGGAAGACATTGCTGCC
CGCCTCAACATCCCAGTCAGCCAGGTGAACCCCAGGGATGCCAAGGCCTG
CGTAGTACACGGCAGTGATCTAAAGGACATGACCTCCGAGCAGCTGGATG
ACATTTTGAAGTACCACACTGAGATAGTGTTTGCCAGGACCTCCCCTCAG
CAGAAGCTCATCATTGTGGAAGGCTGCCAAAGACAGGGTGCTATCGTGGC
TGTGACTGGTGACGGTGTGAATGACTCTCCAGCTTTGAAGAAAGCAGACA
TTGGGGTTGCTATGGGGATTGCTGGCTCAGATGTGTCCAAGCAAGCTGCT
GACATGATTCTTCTGGATGACAACTTTGCCTCAATTGTGACTGGAGTAGA
GGAAGGTCGTCTGATCTTTGATAACTTGAAGAAATCCATTGCTTATACCT
TAACCAGTAACATTCCCGAGATCACCCCGTTCCTGATATTTATTATTGCA
AACATTCCACTACCACTGGGGACTGTCACCATCCTCTGCATTGACTTGGG
CACTGACATGGTTCCTGCCATCTCCCTGGCTTATGAGCAGGCTGAGAGTG
ACATCATGAAGAGACAGCCCAGAAATCCCAAAACAGACAAACTTGTGAAT
GAGCGGCTGATCAGCATGGCCTATGGGCAGATTGGAATGATCCAGGCCCT
GGGAGGCTTCTTTACTTACTTTGTGATTCTGGCTGAGAACGGCTTCCTCC
CAATTCACCTGTTGGGCCTCCGAGTGGACTGGGATGACCGCTGGATCAAC
GATGTGGAAGACAGCTACGGGCAGCAGTGGACCTATGAGCAGAGGAAAAT
CGTGGAGTTCACCTGCCACACAGCCTTCTTCGTCAGTATCGTGGTGGTGC
AGTGGGCCGACTTGGTCATCTGTAAGACCAGGAGGAATTCGGTCTTCCAG
CAGGGGATGAAGAACAAGATCTTGATATTTGGCCTCTTTGAAGAGACAGC
CCTGGCTGCTTTCCTTTCCTACTGCCCTGGAATGGGTGTTGCTCTTAGGA
TGTATCCCCTCAAACCTACCTGGTGGTTCTGTGCCTTCCCCTACTCTCTT
CTCATCTTCGTATATGACGAAGTCAGAAAACTCATCATCAGGCGACGCCC
TGGCGGCTGGGTGGAGAAGGAAACCTACTATGACTACAAGGACGACGATG
ACAAGTAGTCGACAGGATCC

Amino Acid Sequence

MGKGVGRDKYEPAAVSEQGDKKGKKGKKDRDMDELKKEVSMDDHKLSLDE
LHRKYGTDLSRGLTSARAAEILARDGPNALTPPPTTPEWIKFCRQLFGGF
SMLLWIGAILCFLAYSIQAATEEEPQNDNLYLGVVLSAVVIITGCFSYYQ
EAKSSKIMESFKNMVPQQALVIRNGEKMSINAEEVVVGDLVEVKGGDRIP
ADLRIISANGCKVDNSSLTGESEPQTRSPDFTNENPLETRNIAFFSTNCV
EGTARGIVVYTGDRTVMGRIATLASGLEGGQTPIAAEIEHFIHIITGVAV
FLGVSFFILSLILEYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKR
MARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEA
DTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGD
ASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSE
PQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGE
RVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVP
DAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNI
PVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLI
IVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMIL
LDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPL
PLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNERLI
SMAYGQIGMIQALGGFFTYFVILAENGFLPIHLLGLRVDWDDRWINDVED
SYGQQWTYEQRKIVEFTCHTAFFVSIVVVQWADLVICKTRRNSVFQQGMK
NKILIFGLFEETALAAFLSYCPGMGVALRMYPLKPTWWFCAFPYSLLIFV
YDEVRKLIIRRRPGGWVEKETYYDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31843
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee