cDNA Clone Nudel (NDEL1-LLET)

• Plasmid: pGEX6P-2-NDEL1 (Nudel)-LLET
• Parent Plasmid: pGEX6P2
• Expressed: GST-NDEL1 (Nudel)-LLET
• Genbank: AL832648.1 GI:21733224
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGATGGTGAAGATATACCAGATTTTTCAAGTTTAAAGGAAGA
AACTGCTTATTGGAAGGAACTTTCCTTGAAGTATAAGCAAAGCTTCCAGG
AAGCTCGGGATGAGCTAGTTGAATTCCAGGAAGGAAGCAGAGAATTAGAA
GCAGAGTTGGAGGCACAATTAGTACAGGCTGAACAAAGAAATAGAGACTT
GCAGGCTGATAACCAAAGACTGAAATATGAAGTGGAGGCATTAAAGGAGA
AGCTAGAGCATCAATATGCACAGAGCTATAAGCAGGTCTCAGTGTTAGAA
GATGATTTAAGTCAGACTCGGGCCATTAAGGAGCAGTTGCATAAGTATGT
GAGAGAGCTGGAGCAGGCCAACGACGACCTGGAGCGAGCCAAAAGGGCAA
CAATAGTTTCACTGGAAGACTTTGAACAAAGGCTAAACCAGGCCATTGAA
CGAAATGCATTTTTAGAAAGTGAACTTGATGAAAAGGAATCTTTGTTGGT
CTCTGTACAGAGGTTAAAGGATGAAGCAAGAGATTTAAGGCAAGAACTAG
CAGTTCGGGAAAGACAACAGGAAGTAACTAGAAAGTCGGCTCCTAGCTCT
CCAACTCTAGACTGTGAAAAGATGGACTCCGCCGTCCAAGCATCACTTTC
TTTGCCAGCTACCCCTGTTGGCAAAGGAACGGAGAACACTTTTCCTTCAC
CGAAAGCTATACCAAATGGTTTTGGTACCAGTCCACTAACTCCCTCTGCT
AGGATATCAGCACTAAACATCGTGGGGGATCTCTTACGGAAAGTAGGGGC
TTTAGAATCCAAATTAGCAGCTTGCAGGAATTTTGCAAAGGACCAAGCAT
CACGAAAATCCTATATTTCAGGGAATGTTAACTGTGGGGTGCTGAATGGC
AATGGCACAAAGTTCTCTCGATCAGGGCATACATCTTTCTTCGACAAAGG
CTCCTCCTCCAGCTGTGCGATCCGAGCTTCCAGACGCCGCTTCTCATCCA
GCAGCGCGGACCTGGCGGGGAGAGGAGGAGGGGACGGTTCAATCCCAGCT
CAGCACAGCACGGGAGCCTCAGGGAAGGCTCCTCCACCCACCCTGTTGCT
GGAAACCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDGEDIPDFSSLKEETAYW
KELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADN
QRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELE
QANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQR
LKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPAT
PVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESK
LAACRNFAKDQASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGSSSS
CAIRASRRRFSSSSADLAGRGGGDGSIPAQHSTGASGKAPPPTLLLET*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31763
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 67437 Daltons