Plasmid
pGEX6P-2-Atxn1
Parent Plasmid
pGEX6P2
Expressed
GST-Atxn1
Genbank
NM_001128164.1 GI:189491747
Tag
GST
Species
Human
Sequence of Insert
View Sequence
AGATCTATGAAATCCAACCAAGAGCGGAGCAACGAATGCCTGCCTCCCAA
GAAGCGCGAGATCCCCGCCACCAGCCGGTCCTCCGAGGAGAAGGCCCCTA
CCCTGCCCAGCGACAACCACCGGGTGGAGGGCACAGCATGGCTCCCGGGC
AACCCTGGTGGCCGGGGCCACGGGGGCGGGAGGCATGGGCCGGCAGGGAC
CTCGGTGGAGCTTGGTTTACAACAGGGAATAGGTTTACACAAAGCATTGT
CCACAGGGCTGGACTACTCCCCGCCCAGCGCTCCCAGGTCTGTCCCCGTG
GCCACCACGCTGCCTGCCGCGTACGCCACCCCGCAGCCAGGGACCCCGGT
GTCCCCCGTGCAGTACGCTCACCTGCCGCACACCTTCCAGTTCATTGGGT
CCTCCCAATACAGTGGAACCTATGCCAGCTTCATCCCATCACAGCTGATC
CCCCCAACCGCCAACCCCGTCACCAGTGCAGTGGCCTCGGCCGCAGGGGC
CACCACTCCATCCCAGCGCTCCCAGCTGGAGGCCTATTCCACTCTGCTGG
CCAACATGGGCAGTCTGAGCCAGACGCCGGGACACAAGGCTGAGCAGCAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCAGCATCAGCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTCAGCAGGGCTCCGG
GGCTCATCACCCCGGGGTCCCCCCCACCAGCCCAGCAGAACCAGTACGTC
CACATTTCCAGTTCTCCGCAGAACACCGGCCGCACCGCCTCTCCTCCGGC
CATCCCCGTCCACCTCCACCCCCACCAGACGATGATCCCACACACGCTCA
CCCTGGGGCCCCCCTCCCAGGTCGTCATGCAATACGCCGACTCCGGCAGC
CACTTTGTCCCTCGGGAGGCCACCAAGAAAGCTGAGAGCAGCCGGCTGCA
GCAGGCCATCCAGGCCAAGGAGGTCCTGAACGGTGAGATGGAGAAGAGCC
GGCGGTACGGGGCCCCGTCCTCAGCCGACCTGGGCCTGGGCAAGGCAGGC
GGCAAGTCGGTTCCTCACCCGTACGAGTCCAGGCACGTGGTGGTCCACCC
GAGCCCCTCAGACTACAGCAGTCGTGATCCTTCGGGGGTCCGGGCCTCTG
TGATGGTCCTGCCCAACAGCAACACGCCCGCAGCTGACCTGGAGGTGCAA
CAGGCCACTCATCGTGAAGCCTCCCCTTCTACCCTCAACGACAAAAGTGG
CCTGCATTTAGGGAAGCCTGGCCACCGGTCCTACGCGCTCTCACCCCACA
CGGTCATTCAGACCACACACAGTGCTTCAGAGCCACTCCCGGTGGGACTG
CCAGCCACGGCCTTCTACGCAGGGACTCAACCCCCTGTCATCGGCTACCT
GAGCGGCCAGCAGCAAGCAATCACCTACGCCGGCAGCCTGCCCCAGCACC
TGGTGATCCCCGGCACACAGCCCCTGCTCATCCCGGTCGGCAGCACTGAC
ATGGAAGCGTCGGGGGCAGCCCCGGCCATAGTCACGTCATCCCCCCAGTT
TGCTGCAGTGCCTCACACGTTCGTCACCACCGCCCTTCCCAAGAGCGAGA
ACTTCAACCCTGAGGCCCTGGTCACCCAGGCCGCCTACCCAGCCATGGTG
CAGGCCCAGATCCACCTGCCTGTGGTGCAGTCCGTGGCCTCCCCGGCGGC
GGCTCCCCCTACGCTGCCTCCCTACTTCATGAAAGGCTCCATCATCCAGT
TGGCCAACGGGGAGCTAAAGAAGGTGGAAGACTTAAAAACAGAAGATTTC
ATCCAGAGTGCAGAGATAAGCAACGACCTGAAGATCGACTCCAGCACCGT
AGAGAGGATTGAAGACAGCCATAGCCCGGGCGTGGCCGTGATACAGTTCG
CCGTCGGGGAGCACCGAGCCCAGGTCAGCGTTGAAGTTTTGGTAGAGTAT
CCTTTTTTTGTGTTTGGACAGGGCTGGTCATCCTGCTGTCCGGAGAGAAC
CAGCCAGCTCTTTGATTTGCCGTGTTCCAAACTCTCAGTTGGGGATGTCT
GCATCTCGCTTACCCTCAAGAACCTGAAGAACGGCTCTGTTAAAAAGGGC
CAGCCCGTGGATCCCGCCAGCGTCCTGCTGAAGCACTCAAAGGCCGACGG
CCTGGCGGGCAGCAGACACAGGTATGCCGAGCAGGAAAACGGAATCAACC
AGGGGAGTGCCCAGATGCTCTCTGAGAATGGCGAACTGAAGTTTCCAGAG
AAAATGGGATTGCCTGCAGCGCCCTTCCTCACCAAAATAGAACCCAGCAA
GCCCGCGGCAACGAGGAAGAGGAGGTGGTCGGCGCCAGAGAGCCGCAAAC
TGGAGAAGTCAGAAGACGAACCACCTTTGACTCTTCCTAAGCCTTCTCTA
ATTCCTCAGGAGGTTAAGATTTGCATTGAAGGCCGGTCTAATGTAGGCAA
GTAGCGGCCGC
GAAGCGCGAGATCCCCGCCACCAGCCGGTCCTCCGAGGAGAAGGCCCCTA
CCCTGCCCAGCGACAACCACCGGGTGGAGGGCACAGCATGGCTCCCGGGC
AACCCTGGTGGCCGGGGCCACGGGGGCGGGAGGCATGGGCCGGCAGGGAC
CTCGGTGGAGCTTGGTTTACAACAGGGAATAGGTTTACACAAAGCATTGT
CCACAGGGCTGGACTACTCCCCGCCCAGCGCTCCCAGGTCTGTCCCCGTG
GCCACCACGCTGCCTGCCGCGTACGCCACCCCGCAGCCAGGGACCCCGGT
GTCCCCCGTGCAGTACGCTCACCTGCCGCACACCTTCCAGTTCATTGGGT
CCTCCCAATACAGTGGAACCTATGCCAGCTTCATCCCATCACAGCTGATC
CCCCCAACCGCCAACCCCGTCACCAGTGCAGTGGCCTCGGCCGCAGGGGC
CACCACTCCATCCCAGCGCTCCCAGCTGGAGGCCTATTCCACTCTGCTGG
CCAACATGGGCAGTCTGAGCCAGACGCCGGGACACAAGGCTGAGCAGCAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCAGCATCAGCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTCAGCAGGGCTCCGG
GGCTCATCACCCCGGGGTCCCCCCCACCAGCCCAGCAGAACCAGTACGTC
CACATTTCCAGTTCTCCGCAGAACACCGGCCGCACCGCCTCTCCTCCGGC
CATCCCCGTCCACCTCCACCCCCACCAGACGATGATCCCACACACGCTCA
CCCTGGGGCCCCCCTCCCAGGTCGTCATGCAATACGCCGACTCCGGCAGC
CACTTTGTCCCTCGGGAGGCCACCAAGAAAGCTGAGAGCAGCCGGCTGCA
GCAGGCCATCCAGGCCAAGGAGGTCCTGAACGGTGAGATGGAGAAGAGCC
GGCGGTACGGGGCCCCGTCCTCAGCCGACCTGGGCCTGGGCAAGGCAGGC
GGCAAGTCGGTTCCTCACCCGTACGAGTCCAGGCACGTGGTGGTCCACCC
GAGCCCCTCAGACTACAGCAGTCGTGATCCTTCGGGGGTCCGGGCCTCTG
TGATGGTCCTGCCCAACAGCAACACGCCCGCAGCTGACCTGGAGGTGCAA
CAGGCCACTCATCGTGAAGCCTCCCCTTCTACCCTCAACGACAAAAGTGG
CCTGCATTTAGGGAAGCCTGGCCACCGGTCCTACGCGCTCTCACCCCACA
CGGTCATTCAGACCACACACAGTGCTTCAGAGCCACTCCCGGTGGGACTG
CCAGCCACGGCCTTCTACGCAGGGACTCAACCCCCTGTCATCGGCTACCT
GAGCGGCCAGCAGCAAGCAATCACCTACGCCGGCAGCCTGCCCCAGCACC
TGGTGATCCCCGGCACACAGCCCCTGCTCATCCCGGTCGGCAGCACTGAC
ATGGAAGCGTCGGGGGCAGCCCCGGCCATAGTCACGTCATCCCCCCAGTT
TGCTGCAGTGCCTCACACGTTCGTCACCACCGCCCTTCCCAAGAGCGAGA
ACTTCAACCCTGAGGCCCTGGTCACCCAGGCCGCCTACCCAGCCATGGTG
CAGGCCCAGATCCACCTGCCTGTGGTGCAGTCCGTGGCCTCCCCGGCGGC
GGCTCCCCCTACGCTGCCTCCCTACTTCATGAAAGGCTCCATCATCCAGT
TGGCCAACGGGGAGCTAAAGAAGGTGGAAGACTTAAAAACAGAAGATTTC
ATCCAGAGTGCAGAGATAAGCAACGACCTGAAGATCGACTCCAGCACCGT
AGAGAGGATTGAAGACAGCCATAGCCCGGGCGTGGCCGTGATACAGTTCG
CCGTCGGGGAGCACCGAGCCCAGGTCAGCGTTGAAGTTTTGGTAGAGTAT
CCTTTTTTTGTGTTTGGACAGGGCTGGTCATCCTGCTGTCCGGAGAGAAC
CAGCCAGCTCTTTGATTTGCCGTGTTCCAAACTCTCAGTTGGGGATGTCT
GCATCTCGCTTACCCTCAAGAACCTGAAGAACGGCTCTGTTAAAAAGGGC
CAGCCCGTGGATCCCGCCAGCGTCCTGCTGAAGCACTCAAAGGCCGACGG
CCTGGCGGGCAGCAGACACAGGTATGCCGAGCAGGAAAACGGAATCAACC
AGGGGAGTGCCCAGATGCTCTCTGAGAATGGCGAACTGAAGTTTCCAGAG
AAAATGGGATTGCCTGCAGCGCCCTTCCTCACCAAAATAGAACCCAGCAA
GCCCGCGGCAACGAGGAAGAGGAGGTGGTCGGCGCCAGAGAGCCGCAAAC
TGGAGAAGTCAGAAGACGAACCACCTTTGACTCTTCCTAAGCCTTCTCTA
ATTCCTCAGGAGGTTAAGATTTGCATTGAAGGCCGGTCTAATGTAGGCAA
GTAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKSNQERSNECLPPKKREI
PATSRSSEEKAPTLPSDNHRVEGTAWLPGNPGGRGHGGGRHGPAGTSVEL
GLQQGIGLHKALSTGLDYSPPSAPRSVPVATTLPAAYATPQPGTPVSPVQ
YAHLPHTFQFIGSSQYSGTYASFIPSQLIPPTANPVTSAVASAAGATTPS
QRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQQQHQHQQQQQQQQ
QQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQNTGRTASPPAIPVH
LHPHQTMIPHTLTLGPPSQVVMQYADSGSHFVPREATKKAESSRLQQAIQ
AKEVLNGEMEKSRRYGAPSSADLGLGKAGGKSVPHPYESRHVVVHPSPSD
YSSRDPSGVRASVMVLPNSNTPAADLEVQQATHREASPSTLNDKSGLHLG
KPGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAGTQPPVIGYLSGQQ
QAITYAGSLPQHLVIPGTQPLLIPVGSTDMEASGAAPAIVTSSPQFAAVP
HTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPAAAPPT
LPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIE
DSHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLF
DLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKHSKADGLAGS
RHRYAEQENGINQGSAQMLSENGELKFPEKMGLPAAPFLTKIEPSKPAAT
RKRRWSAPESRKLEKSEDEPPLTLPKPSLIPQEVKICIEGRSNVGK*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKSNQERSNECLPPKKREI
PATSRSSEEKAPTLPSDNHRVEGTAWLPGNPGGRGHGGGRHGPAGTSVEL
GLQQGIGLHKALSTGLDYSPPSAPRSVPVATTLPAAYATPQPGTPVSPVQ
YAHLPHTFQFIGSSQYSGTYASFIPSQLIPPTANPVTSAVASAAGATTPS
QRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQQQHQHQQQQQQQQ
QQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQNTGRTASPPAIPVH
LHPHQTMIPHTLTLGPPSQVVMQYADSGSHFVPREATKKAESSRLQQAIQ
AKEVLNGEMEKSRRYGAPSSADLGLGKAGGKSVPHPYESRHVVVHPSPSD
YSSRDPSGVRASVMVLPNSNTPAADLEVQQATHREASPSTLNDKSGLHLG
KPGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAGTQPPVIGYLSGQQ
QAITYAGSLPQHLVIPGTQPLLIPVGSTDMEASGAAPAIVTSSPQFAAVP
HTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPAAAPPT
LPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIE
DSHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLF
DLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKHSKADGLAGS
RHRYAEQENGINQGSAQMLSENGELKFPEKMGLPAAPFLTKIEPSKPAAT
RKRRWSAPESRKLEKSEDEPPLTLPKPSLIPQEVKICIEGRSNVGK*
Vector Type
Bacterial
Antibiotic
Amp
DU Number
DU31668
Unit Source
Location
PPU
MTA
Notes
Bacterial construct to express GST N terminal fusion
Estimated MW of expressed protein : 113741 Daltons
Estimated MW of expressed protein : 113741 Daltons
Price
£125.00
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