cDNA Clone Atxn1

• Plasmid: pGEX6P-2-Atxn1
• Parent Plasmid: pGEX6P2
• Expressed: GST-Atxn1
• Genbank: NM_001128164.1 GI:189491747
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGAAATCCAACCAAGAGCGGAGCAACGAATGCCTGCCTCCCAA
GAAGCGCGAGATCCCCGCCACCAGCCGGTCCTCCGAGGAGAAGGCCCCTA
CCCTGCCCAGCGACAACCACCGGGTGGAGGGCACAGCATGGCTCCCGGGC
AACCCTGGTGGCCGGGGCCACGGGGGCGGGAGGCATGGGCCGGCAGGGAC
CTCGGTGGAGCTTGGTTTACAACAGGGAATAGGTTTACACAAAGCATTGT
CCACAGGGCTGGACTACTCCCCGCCCAGCGCTCCCAGGTCTGTCCCCGTG
GCCACCACGCTGCCTGCCGCGTACGCCACCCCGCAGCCAGGGACCCCGGT
GTCCCCCGTGCAGTACGCTCACCTGCCGCACACCTTCCAGTTCATTGGGT
CCTCCCAATACAGTGGAACCTATGCCAGCTTCATCCCATCACAGCTGATC
CCCCCAACCGCCAACCCCGTCACCAGTGCAGTGGCCTCGGCCGCAGGGGC
CACCACTCCATCCCAGCGCTCCCAGCTGGAGGCCTATTCCACTCTGCTGG
CCAACATGGGCAGTCTGAGCCAGACGCCGGGACACAAGGCTGAGCAGCAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCAGCATCAGCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTCAGCAGGGCTCCGG
GGCTCATCACCCCGGGGTCCCCCCCACCAGCCCAGCAGAACCAGTACGTC
CACATTTCCAGTTCTCCGCAGAACACCGGCCGCACCGCCTCTCCTCCGGC
CATCCCCGTCCACCTCCACCCCCACCAGACGATGATCCCACACACGCTCA
CCCTGGGGCCCCCCTCCCAGGTCGTCATGCAATACGCCGACTCCGGCAGC
CACTTTGTCCCTCGGGAGGCCACCAAGAAAGCTGAGAGCAGCCGGCTGCA
GCAGGCCATCCAGGCCAAGGAGGTCCTGAACGGTGAGATGGAGAAGAGCC
GGCGGTACGGGGCCCCGTCCTCAGCCGACCTGGGCCTGGGCAAGGCAGGC
GGCAAGTCGGTTCCTCACCCGTACGAGTCCAGGCACGTGGTGGTCCACCC
GAGCCCCTCAGACTACAGCAGTCGTGATCCTTCGGGGGTCCGGGCCTCTG
TGATGGTCCTGCCCAACAGCAACACGCCCGCAGCTGACCTGGAGGTGCAA
CAGGCCACTCATCGTGAAGCCTCCCCTTCTACCCTCAACGACAAAAGTGG
CCTGCATTTAGGGAAGCCTGGCCACCGGTCCTACGCGCTCTCACCCCACA
CGGTCATTCAGACCACACACAGTGCTTCAGAGCCACTCCCGGTGGGACTG
CCAGCCACGGCCTTCTACGCAGGGACTCAACCCCCTGTCATCGGCTACCT
GAGCGGCCAGCAGCAAGCAATCACCTACGCCGGCAGCCTGCCCCAGCACC
TGGTGATCCCCGGCACACAGCCCCTGCTCATCCCGGTCGGCAGCACTGAC
ATGGAAGCGTCGGGGGCAGCCCCGGCCATAGTCACGTCATCCCCCCAGTT
TGCTGCAGTGCCTCACACGTTCGTCACCACCGCCCTTCCCAAGAGCGAGA
ACTTCAACCCTGAGGCCCTGGTCACCCAGGCCGCCTACCCAGCCATGGTG
CAGGCCCAGATCCACCTGCCTGTGGTGCAGTCCGTGGCCTCCCCGGCGGC
GGCTCCCCCTACGCTGCCTCCCTACTTCATGAAAGGCTCCATCATCCAGT
TGGCCAACGGGGAGCTAAAGAAGGTGGAAGACTTAAAAACAGAAGATTTC
ATCCAGAGTGCAGAGATAAGCAACGACCTGAAGATCGACTCCAGCACCGT
AGAGAGGATTGAAGACAGCCATAGCCCGGGCGTGGCCGTGATACAGTTCG
CCGTCGGGGAGCACCGAGCCCAGGTCAGCGTTGAAGTTTTGGTAGAGTAT
CCTTTTTTTGTGTTTGGACAGGGCTGGTCATCCTGCTGTCCGGAGAGAAC
CAGCCAGCTCTTTGATTTGCCGTGTTCCAAACTCTCAGTTGGGGATGTCT
GCATCTCGCTTACCCTCAAGAACCTGAAGAACGGCTCTGTTAAAAAGGGC
CAGCCCGTGGATCCCGCCAGCGTCCTGCTGAAGCACTCAAAGGCCGACGG
CCTGGCGGGCAGCAGACACAGGTATGCCGAGCAGGAAAACGGAATCAACC
AGGGGAGTGCCCAGATGCTCTCTGAGAATGGCGAACTGAAGTTTCCAGAG
AAAATGGGATTGCCTGCAGCGCCCTTCCTCACCAAAATAGAACCCAGCAA
GCCCGCGGCAACGAGGAAGAGGAGGTGGTCGGCGCCAGAGAGCCGCAAAC
TGGAGAAGTCAGAAGACGAACCACCTTTGACTCTTCCTAAGCCTTCTCTA
ATTCCTCAGGAGGTTAAGATTTGCATTGAAGGCCGGTCTAATGTAGGCAA
GTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKSNQERSNECLPPKKREI
PATSRSSEEKAPTLPSDNHRVEGTAWLPGNPGGRGHGGGRHGPAGTSVEL
GLQQGIGLHKALSTGLDYSPPSAPRSVPVATTLPAAYATPQPGTPVSPVQ
YAHLPHTFQFIGSSQYSGTYASFIPSQLIPPTANPVTSAVASAAGATTPS
QRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQQQHQHQQQQQQQQ
QQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQNTGRTASPPAIPVH
LHPHQTMIPHTLTLGPPSQVVMQYADSGSHFVPREATKKAESSRLQQAIQ
AKEVLNGEMEKSRRYGAPSSADLGLGKAGGKSVPHPYESRHVVVHPSPSD
YSSRDPSGVRASVMVLPNSNTPAADLEVQQATHREASPSTLNDKSGLHLG
KPGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAGTQPPVIGYLSGQQ
QAITYAGSLPQHLVIPGTQPLLIPVGSTDMEASGAAPAIVTSSPQFAAVP
HTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPAAAPPT
LPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIE
DSHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLF
DLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKHSKADGLAGS
RHRYAEQENGINQGSAQMLSENGELKFPEKMGLPAAPFLTKIEPSKPAAT
RKRRWSAPESRKLEKSEDEPPLTLPKPSLIPQEVKICIEGRSNVGK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31668
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 113741 Daltons