cDNA Clone ETV1

• Plasmid: pGEX6P-2-ETV1
• Parent Plasmid: pGEX6P2
• Expressed: GST-ETV1
• Genbank: NM_004956.3 GI:64368770*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGATGGATTTTATGACCAGCAAGTGCCTTACATGGTCACCAA
TAGTCAGCGTGGGAGAAATTGTAACGAGAAACCAACAAATGTCAGGAAAA
GAAAATTCATTGACAGAGATCTGGCTCATGATTCAGAAGAACTCTTTCAA
GATCTAAGTCAATTACAGGAAACATGGCTTGCAGAAGCTCAGGTACCTGA
CAATGATGAGCAGTTTGTACCAGACTATCAGGCTGAAAGTTTGGCTTTTC
ATGGCCTGCCACTGAAAATCAAGAAAGAACCCCACAGTCCATGTTCAGAA
ATCAGCTCTGCCTGCAGTCAAGAACAGCCCTTTAAATTCAGCTATGGAGA
AAAGTGCCTGTACAATGTCAGTGCCTATGATCAGAAGCCACAAGTGGGAA
TGAGGCCCTCCAACCCCCCCACACCATCCAGCACGCCAGTGTCCCCACTG
CATCATGCATCTCCAAACTCAACTCATACACCGAAACCTGACCGGGCCTT
CCCAGCTCACCTCCCTCCATCGCAGTCCATACCAGATAGCAGCTACCCCA
TGGACCACAGATTTCGCCGCCAGCTTTCTGAACCCTGTAACTCCTTTCCT
CCTTTGCCGACGATGCCAAGGGAAGGACGTCCTATGTACCAACGCCAGAT
GTCTGAGCCAAACATCCCCTTCCCACCACAAGGCTTTAAGCAGGAGTACC
ACGACCCAGTGTATGAACACAACACCATGGTTGGCAGTGCGGCCAGCCAA
AGCTTTCCCCCTCCTCTGATGATTAAACAGGAACCCAGAGATTTTGCATA
TGACTCAGAAGTGCCTAGCTGCCACTCCATTTATATGAGGCAAGAAGGCT
TCCTGGCTCATCCCAGCAGAACAGAAGGCTGTATGTTTGAAAAGGGCCCC
AGGCAGTTTTATGATGACACCTGTGTTGTCCCAGAAAAATTCGATGGAGA
CATCAAACAAGAGCCAGGAATGTATCGGGAAGGACCCACATACCAACGGC
GAGGATCACTTCAGCTCTGGCAGTTTTTGGTAGCTCTTCTGGATGACCCT
TCAAATTCTCATTTTATTGCCTGGACTGGTCGAGGCATGGAATTTAAACT
GATTGAGCCTGAAGAGGTGGCCCGACGTTGGGGCATTCAGAAAAACAGGC
CAGCTATGAACTATGATAAACTTAGCCGTTCACTCCGCTATTACTATGAG
AAAGGAATTATGCAAAAGGTGGCTGGAGAGAGATATGTCTACAAGTTTGT
GTGTGATCCAGAAGCCCTTTTCTCCATGGCCTTTCCAGATAATCAGCGTC
CACTGCTGAAGACAGACATGGAACGTCACATCAACGAGGAGGACACAGTG
CCTCTTTCTCACTTTGATGAGAGCATGGCCTACATGCCGGAAGGGGGCCG
CTGCAACCCCCACCCCTACAACGAAGGCTACGTGTATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDGFYDQQVPYMVTNSQRG
RNCNEKPTNVRKRKFIDRDLAHDSEELFQDLSQLQETWLAEAQVPDNDEQ
FVPDYQAESLAFHGLPLKIKKEPHSPCSEISSACSQEQPFKFSYGEKCLY
NVSAYDQKPQVGMRPSNPPTPSSTPVSPLHHASPNSTHTPKPDRAFPAHL
PPSQSIPDSSYPMDHRFRRQLSEPCNSFPPLPTMPREGRPMYQRQMSEPN
IPFPPQGFKQEYHDPVYEHNTMVGSAASQSFPPPLMIKQEPRDFAYDSEV
PSCHSIYMRQEGFLAHPSRTEGCMFEKGPRQFYDDTCVVPEKFDGDIKQE
PGMYREGPTYQRRGSLQLWQFLVALLDDPSNSHFIAWTGRGMEFKLIEPE
EVARRWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCDPE
ALFSMAFPDNQRPLLKTDMERHINEEDTVPLSHFDESMAYMPEGGRCNPH
PYNEGYVY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31657
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * Sequence differences compared with NM_004956.3 GI:64368770 CDS:; a106g (N36D), t1393c (C465R) ; These changes derive from MP clone DU1522; Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 82007 Daltons