cDNA Clone IRS2 (mouse)

• Plasmid: pcDNA5-FRT/TO-HA IRS2 (mouse)
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA-IRS2 (mouse)
• Genbank: NM_001081212.1 GI:124487072
• Tag: HA
• Species: Mouse

Sequence of Insert

GGATCCATGGCTAGCGCGCCCCTGCCTGGGCCCCCCGCGTCGGCGGGCGG
GGACGGCCCGAACCTCAATAACAACAACAACAACAACAACCACAGCGTGC
GCAAGTGCGGCTACCTGCGCAAGCAGAAGCACGGCCACAAGCGCTTTTTC
GTGTTGCGCGGCCCCGGCACGGGCGGCGACGAGGCATCCGCGGCTGGGGG
GTCGCCGCCGCAGCCTCCGCGGCTGGAGTACTACGAGAGCGAGAAGAAGT
GGAGGAGCAAGGCGGGCGCGCCGAAGCGAGTGATCGCGCTCGACTGCTGT
CTGAACATCAACAAGCGCGCGGACGCCAAGCACAAGTACCTGATCGCCCT
CTACACCAAGGACGAGTACTTCGCTGTAGCGGCGGAGAACGAGCAGGAGC
AGGAGGGCTGGTACCGCGCACTCACCGACTTGGTCAGCGAAGGCCGCTCT
GGCGAGGGGGGCTCGGGCACCACCGGAGGCTCTTGCAGCGCCTCTCTCCC
GGGCGTCCTGGGCGGCTCAGCGGGCGCCGCTGGCTGCGATGACAACTACG
GGCTCGTGACACCCGCCACGGCCGTCTACCGCGAGGTGTGGCAGGTGAAC
CTGAAACCTAAGGGACTGGGCCAGAGCAAGAACCTGACTGGTGTATACCG
CCTATGCCTGTCTGCGCGCACCATCGGCTTCGTGAAGCTCAATTGCGAAC
AGCCGTCGGTGACGCTGCAGCTTATGAACATTCGCCGCTGCGGCCACTCG
GACAGCTTCTTCTTCATCGAGGTGGGCCGTTCGGCCGTCACCGGTCCCGG
GGAGCTGTGGATGCAAGCCGACGACTCGGTGGTGGCGCAGAACATCCATG
AGACCATCCTAGAAGCTATGAAGGCACTCAAAGAGCTCTTCGAGTTCCGG
CCTCGCAGCAAGAGTCAGTCGTCCGGGTCGTCAGCCACGCATCCCATCAG
CGTGCCGGGCGCGCGCCGCCACCACCACCTAGTCAACCTACCCCCTAGCC
AGACCGGCCTGGTGCGCCGCTCGCGCACTGACAGCCTGGCGGCCACCCCC
CCAGCAGCCAAGTGCACTTCGTGCCGGGTTCGTACGGCCAGCGAGGGCGA
CGGCGGCGCGGCAGGCGGGGCCGGGACGGCAGGAGGCAGGCCGATGTCGG
TGGCAGGGAGCCCCCTGAGTCCCGGGCCGGTGCGCGCGCCCCTTAGCCGC
TCGCACACCCTGAGCGCCGGCTGCGGAGGCCGCCCGAGCAAAGTGACTCT
GGCGCCGGCAGGGGGAGCCCTGCAACACAGCCGCTCCATGTCCATGCCCG
TGGCGCACTCACCTCCTGCAGCCACCAGCCCAGGCAGCCTGTCCTCCAGC
AGTGGGCACGGCTCGGGCTCCTACCCGCTGCCACCTGGCTCCCACCCGCA
CCTGCCTCATCCACTGCATCACCCCCAAGGCCAGCGTCCGTCCAGCGGTA
GTGCCTCCGCCTCGGGCTCCCCCAGCGACCCGGGTTTCATGTCCCTTGAC
GAGTATGGCTCCAGCCCTGGCGACCTGAGAGCCTTCAGTAGCCACAGGAG
CAACACACCCGAGTCAATAGCGGAGACCCCGCCAGCCAGAGATGGCAGTG
GGGGCGAACTCTATGGGTACATGAGCATGGATAGACCCCTGAGCCACTGT
GGCCGCCCTTACCGTAGGGTCTCAGGGGATGGGGCCCAGGACCTGGATAG
AGGACTGAGGAAGAGGACTTATTCCCTAACCACGCCTGCCAGGCAGCGGC
AGGTACCTCAGCCTTCCTCTGCCTCTCTAGATGAATACACTCTCATGAGG
GCCACCTTCTCTGGTAGTTCAGGTCGCCTCTGCCCATCCTTCCCTGCGTC
CTCTCCCAAAGTGGCCTACAACCCTTACCCAGAGGACTATGGAGACATTG
AGATTGGTTCTCACAAGAGTTCCAGCAGTAACCTGGGGGCAGATGATGGC
TACATGCCCATGACCCCTGGGGCAGCCCTTAGGAGTGGTGGTCCCAATAG
CTGCAAGAGCGATGACTACATGCCCATGAGCCCCACAAGCGTGTCTGCTC
CCAAGCAGATCCTGCAGCCACGCTTGGCAGCGGCCTTGCCCCCTTCCGGA
GCAGCCGTGCCAGCACCCCCTTCAGGGGTGGGCAGGACCTTCCCAGTAAA
CGGAGGTGGCTACAAAGCCAGCTCCCCAGCGGAGAGCTCCCCAGAAGACA
GTGGGTACATGCGAATGTGGTGTGGCTCCAAGCTGTCTATGGAGAACCCA
GACCCTAAGCTACTCCCCAACGGGGACTACCTCAACATGTCCCCCAGCGA
GGCAGGCACTGCAGGGACCCCACCTGACTTCTCAGCAGCTTTGCGTGGAG
GCAGTGAAGGCCTCAAAGGCATCCCGGGCCACTGCTACAGCTCTTTGCCC
CGCTCTTATAAGGCTCCCTGTTCCTGCAGCGGAGACAATGACCAGTATGT
GCTCATGAGCTCCCCTGTGGGCCGGATCTTGGAAGAGGAGAGACTGGAGC
CCCAGGCCACCCCAGGGGCTGGCACCTTTGGGGCAGCTGGTGGTAGTCAT
ACCCAGCCTCATCACTCAGCAGTGCCTTCCTCCATGAGGCCGAGTGCCAT
CGGTGGCCGCCCTGAGGGCTTCCTGGGCCAGCGATGTCGGGCAGTGCGGC
CTACACGCCTATCGCTAGAGGGACTGCAGACCCTTCCCAGCATGCAAGAG
TACCCTCTACCCACAGAGCCCAAGAGCCCTGGCGAGTACATCAACATTGA
CTTTGGTGAGGCAGGTACCCGTCTGTCTCCGCCTGCCCCCCCACTACTGG
CATCCGCGGCCTCATCTTCTTCACTGCTCTCAGCTAGTAGTCCTGCTTCA
TCCCTGGGTTCAGGAACCCCAGGCACCAGCAGCGACAGCCGGCAGCGCTC
TCCACTCTCTGACTATATGAACCTGGACTTCAGTTCTCCCAAGTCCCCCA
AGCCTAGCACCCGCAGTGGGGACACAGTAGGCTCCATGGATGGCCTTCTC
TCTCCAGAGGCTTCATCCCCATACCCACCACTGCCCCCACGTCCTTCCAC
TTCCCCTTCCTCCTTACAGCAGCCTCTGCCACCTGCCCCGGGAGACCTAT
ACCGCCTGCCTCCAGCATCAGCTGCCACTTCCCAGGGTCCCACTGCTGGC
TCCTCAATGTCCTCCGAGCCTGGGGATAATGGTGACTATACCGAGATGGC
CTTTGGTGTGGCTGCAACCCCGCCACAACCTATCGTGGCACCTCCAAAGC
CAGAAGGTGCCCGAGTGGCCAGTCCCACATCGGGCTTGAAGCGGCTAAGT
CTCATGGATCAGGTATCTGGGGTGGAGGCTTTCCTTCAAGTCAGCCAGCC
CCCTGACCCCCACCGGGGTGCTAAGGTCATCCGTGCAGACCCACAGGGGG
GACGTCGTCGCCACAGTTCAGAGACCTTTTCCTCTACCACCACCGTCACC
CCAGTGTCCCCATCCTTTGCCCACAATTCCAAGCGCCACAATTCGGCCTC
TGTGGAAAATGTCTCACTCAGGAAAAGCAGTGAAGGCAGCAGTACCCTGG
GAGGAGGTGATGAGCCGCCCACATCCCCAGGACAGGCACAGCCCTTGGTG
GCTGTGCCCCCAGTGCCACAGGCTAGGCCGTGGAACCCCGGTCAGCCCGG
AGCTTTGATTGGCTGTCCTGGAGGCAGCAGTTCTCCCATGCGCAGAGAGA
CCTCCGTGGGTTTCCAGAACGGCCTCAACTATATCGCCATCGATGTGAGA
GGCGAGCAGGGGTCCTTGGCGCAGTCTCAGCCGCAGCCAGGAGACAAGAA
CTCCTGGAGCCGGACCCGTAGCCTTGGGGGGCTCCTCGGCACCGTCGGAG
GCTCTGGCGCCAGCGGAGTGTGTGGGGGTCCAGGCACTGGAGCTTTGCCC
TCTGCCAGCACCTATGCAAGCATCGACTTCCTGTCCCATCACTTGAAGGA
AGCCACAGTCGTGAAAGAGTGACTCGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMASAPLPGPPASAGGDGPNLNNNNNNNNHSVRKCGYLR
KQKHGHKRFFVLRGPGTGGDEASAAGGSPPQPPRLEYYESEKKWRSKAGA
PKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRA
LTDLVSEGRSGEGGSGTTGGSCSASLPGVLGGSAGAAGCDDNYGLVTPAT
AVYREVWQVNLKPKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQ
LMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAM
KALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRR
SRTDSLAATPPAAKCTSCRVRTASEGDGGAAGGAGTAGGRPMSVAGSPLS
PGPVRAPLSRSHTLSAGCGGRPSKVTLAPAGGALQHSRSMSMPVAHSPPA
ATSPGSLSSSSGHGSGSYPLPPGSHPHLPHPLHHPQGQRPSSGSASASGS
PSDPGFMSLDEYGSSPGDLRAFSSHRSNTPESIAETPPARDGSGGELYGY
MSMDRPLSHCGRPYRRVSGDGAQDLDRGLRKRTYSLTTPARQRQVPQPSS
ASLDEYTLMRATFSGSSGRLCPSFPASSPKVAYNPYPEDYGDIEIGSHKS
SSSNLGADDGYMPMTPGAALRSGGPNSCKSDDYMPMSPTSVSAPKQILQP
RLAAALPPSGAAVPAPPSGVGRTFPVNGGGYKASSPAESSPEDSGYMRMW
CGSKLSMENPDPKLLPNGDYLNMSPSEAGTAGTPPDFSAALRGGSEGLKG
IPGHCYSSLPRSYKAPCSCSGDNDQYVLMSSPVGRILEEERLEPQATPGA
GTFGAAGGSHTQPHHSAVPSSMRPSAIGGRPEGFLGQRCRAVRPTRLSLE
GLQTLPSMQEYPLPTEPKSPGEYINIDFGEAGTRLSPPAPPLLASAASSS
SLLSASSPASSLGSGTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPSTRSG
DTVGSMDGLLSPEASSPYPPLPPRPSTSPSSLQQPLPPAPGDLYRLPPAS
AATSQGPTAGSSMSSEPGDNGDYTEMAFGVAATPPQPIVAPPKPEGARVA
SPTSGLKRLSLMDQVSGVEAFLQVSQPPDPHRGAKVIRADPQGGRRRHSS
ETFSSTTTVTPVSPSFAHNSKRHNSASVENVSLRKSSEGSSTLGGGDEPP
TSPGQAQPLVAVPPVPQARPWNPGQPGALIGCPGGSSSPMRRETSVGFQN
GLNYIAIDVRGEQGSLAQSQPQPGDKNSWSRTRSLGGLLGTVGGSGASGV
CGGPGTGALPSASTYASIDFLSHHLKEATVVKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31638
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee