cDNA Clone Rhotekin

• Plasmid: pGEX6P-2-Rhotekin R7-L89
• Parent Plasmid: pGEX6P2
• Expressed: GST-Rhotekin R7-L89
• Genbank: NM_001015055.1 GI:62739178
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCGGAGCCGGGTCACCGTGGCCAGGGGCTCCGCCCTGGAGATGGA
GTTCAAACGCGGCCGCTTCCGACTCAGCCTCTTCAGCGACCTGCCCGAGG
ACACGGAGTTGCAGAGGAAGCTAGACCATGAGATCCGGATGAGGGAAGGG
GCCTGTAAGCTGCTGGCAGCCTGCTCCCAGCGAGAGCAGGCTCTGGAGGC
CACCAAGAGCCTGCTAGTGTGCAACAGCCGCATCCTCAGCTACATGGGCG
AGCTGTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRSRVTVARGSALEMEFKRG
RFRLSLFSDLPEDTELQRKLDHEIRMREGACKLLAACSQREQALEATKSL
LVCNSRILSYMGEL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31622
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 36340 Daltons