cDNA Clone NDE1-KRHS

• Plasmid: pCMV5-HA NDE1-KRHS
• Parent Plasmid: pCMV5 HA1
• Expressed: HA NDE1-KRHS
• Genbank: *
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGAGGACTCCGGAAAGACTTTCAGCTCCGAGGAGGAAGAAGC
TAACTATTGGAAAGATCTGGCGATGACCTACAAACAGAGGGCAGAAAATA
CGCAAGAGGAACTCCGAGAATTCCAGGAGGGAAGCCGAGAATATGAAGCT
GAATTGGAGACGCAGCTGCAACAAATTGAAACCAGGAACAGAGACCTCCT
GTCCGAAAATAACCGCCTTCGCATGGAGCTGGAAACCATCAAGGAGAAGT
TTGAAGTGCAGCACTCTGAAGGCTACCGGCAGATCTCAGCCTTGGAGGAT
GACCTCGCGCAGACCAAAGCCATTAAAGACCAATTGCAGAAATACATCAG
AGAGCTGGAGCAAGCAAATGACGACCTGGAAAGAGCCAAGCGCGCCACGA
TCATGTCTCTCGAAGACTTTGAGCAGCGCTTGAATCAGGCCATCGAAAGA
AATGCCTTCCTGGAAAGTGAACTTGATGAAAAAGAGAATCTCCTGGAATC
TGTTCAGAGACTGAAGGATGAAGCCAGAGATTTGCGGCAGGAACTGGCCG
TGCAGCAGAAGCAGGAGAAACCCAGGACCCCCATGCCCAGCTCAGTGGAA
GCTGAGAGGACAGACACAGCTGTGCAGGCCACGGGCTCCGTGCCGTCCAC
GCCCATTGCTCACCGAGGACCCAGCTCAAGTTTAAACACACCTGGGAGCT
TCAGACGTGGCCTGGACGACTCCACCGGGGGGACCCCCCTCACACCTGCG
GCCCGGATATCAGCCCTCAACATTGTGGGAGACCTACTGCGGAAAGTCGG
GGCACTGGAGTCCAAACTCGCTTCCTGCCGGAACCTCGTGTACGATCAGT
CCCCAAACCGAACAGGTGGCCCAGCCTCTGGGCGGAGCAGCAAGAACAGA
GATGGCGGGGAGAGACGGCCAAGCAGCACCAGCGTGCCTTTGGGTGATAA
GGGGTCCGTGCCCAGCAACAAACCCCTGGCCGGGGGCGAAAACCCCCCTG
CCCCCGGGAAGCGGCACTCCTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELRE
FQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSE
GYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDF
EQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEK
PRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDD
STGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGG
PASGRSSKNRDGGERRPSSTSVPLGDKGSVPSNKPLAGGENPPAPGKRHS
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31610
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: This isoform of NDE1 has been predicted as a splice variant in the paper below. Transcripts were detected in various human brain regions. It is a 338 aa protein ending in KRHS.; ; Bradshaw NJ et al 2009. NDE1 and NDEL1: multimerisation, alternate splicing and DISC1 interaction. Neurosci Lett. 449:228-233 ; ; Estimated MW of expressed protein : 39170 Daltons