cDNA Clone Klotho beta

• Synonyms: KLB, BKL, MGC142213
• Plasmid: pcDNA5-FRT/TO Klotho beta-GFP
• Parent Plasmid: pcDNA5 FRT/TO C-GFP
• Expressed: Klotho beta-GFP
• Genbank: NM_175737.3 GI:198041706
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGAAGCCAGGCTGTGCGGCAGGATCTCCAGGGAATGA
ATGGATTTTCTTCAGCACTGATGAAATAACCACACGCTATAGGAATACAA
TGTCCAACGGGGGATTGCAAAGATCTGTCATCCTGTCAGCACTTATTCTG
CTACGAGCTGTTACTGGATTCTCTGGAGATGGAAGAGCTATATGGTCTAA
AAATCCTAATTTTACTCCGGTAAATGAAAGTCAGCTGTTTCTCTATGACA
CTTTCCCTAAAAACTTTTTCTGGGGTATTGGGACTGGAGCATTGCAAGTG
GAAGGGAGTTGGAAGAAGGATGGAAAAGGACCTTCTATATGGGATCATTT
CATCCACACACACCTTAAAAATGTCAGCAGCACGAATGGTTCCAGTGACA
GTTATATTTTTCTGGAAAAAGACTTATCAGCCCTGGATTTTATAGGAGTT
TCTTTTTATCAATTTTCAATTTCCTGGCCAAGGCTTTTCCCCGATGGAAT
AGTAACAGTTGCCAACGCAAAAGGTCTGCAGTACTACAGTACTCTTCTGG
ACGCTCTAGTGCTTAGAAACATTGAACCTATAGTTACTTTATACCACTGG
GATTTGCCTTTGGCACTACAAGAAAAATATGGGGGGTGGAAAAATGATAC
CATAATAGATATCTTCAATGACTATGCCACATACTGTTTCCAGATGTTTG
GGGACCGTGTCAAATATTGGATTACAATTCACAACCCATATCTAGTGGCT
TGGCATGGGTATGGGACAGGTATGCATGCCCCTGGAGAGAAGGGAAATTT
AGCAGCTGTCTACACTGTGGGACACAACTTGATCAAGGCTCACTCGAAAG
TTTGGCATAACTACAACACACATTTCCGCCCACATCAGAAGGGTTGGTTA
TCGATCACGTTGGGATCTCATTGGATCGAGCCAAACCGGTCGGAAAACAC
GATGGATATATTCAAATGTCAACAATCCATGGTTTCTGTGCTTGGATGGT
TTGCCAACCCTATCCATGGGGATGGCGACTATCCAGAGGGGATGAGAAAG
AAGTTGTTCTCCGTTCTACCCATTTTCTCTGAAGCAGAGAAGCATGAGAT
GAGAGGCACAGCTGATTTCTTTGCCTTTTCTTTTGGACCCAACAACTTCA
AGCCCCTAAACACCATGGCTAAAATGGGACAAAATGTTTCACTTAATTTA
AGAGAAGCGCTGAACTGGATTAAACTGGAATACAACAACCCTCGAATCTT
GATTGCTGAGAATGGCTGGTTCACAGACAGTCGTGTGAAAACAGAAGACA
CCACGGCCATCTACATGATGAAGAATTTCCTCAGCCAGGTGCTTCAAGCA
ATAAGGTTAGATGAAATACGAGTGTTTGGTTATACTGCCTGGTCTCTCCT
GGATGGCTTTGAATGGCAGGATGCTTACACCATCCGCCGAGGATTATTTT
ATGTGGATTTTAACAGTAAACAGAAAGAGCGGAAACCTAAGTCTTCAGCA
CACTACTACAAACAGATCATACGAGAAAATGGTTTTTCTTTAAAAGAGTC
CACGCCAGATGTGCAGGGCCAGTTTCCCTGTGACTTCTCCTGGGGTGTCA
CTGAATCTGTTCTTAAGCCCGAGTCTGTGGCTTCGTCCCCACAGTTCAGC
GATCCTCATCTGTACGTGTGGAACGCCACTGGCAACAGACTGTTGCACCG
AGTGGAAGGGGTGAGGCTGAAAACACGACCCGCTCAATGCACAGATTTTG
TAAACATCAAAAAACAACTTGAGATGTTGGCAAGAATGAAAGTCACCCAC
TACCGGTTTGCTCTGGATTGGGCCTCGGTCCTTCCCACTGGCAACCTGTC
CGCGGTGAACCGACAGGCCCTGAGGTACTACAGGTGCGTGGTCAGTGAGG
GGCTGAAGCTTGGCATCTCCGCGATGGTCACCCTGTATTATCCGACCCAC
GCCCACCTAGGCCTCCCCGAGCCTCTGTTGCATGCCGACGGGTGGCTGAA
CCCATCGACGGCCGAGGCCTTCCAGGCCTACGCTGGGCTGTGCTTCCAGG
AGCTGGGGGACCTGGTGAAGCTCTGGATCACCATCAACGAGCCTAACCGG
CTAAGTGACATCTACAACCGCTCTGGCAACGACACCTACGGGGCGGCGCA
CAACCTGCTGGTGGCCCACGCCCTGGCCTGGCGCCTCTACGACCGGCAGT
TCAGGCCCTCACAGCGCGGGGCCGTGTCGCTGTCGCTGCACGCGGACTGG
GCGGAACCCGCCAACCCCTATGCTGACTCGCACTGGAGGGCGGCCGAGCG
CTTCCTGCAGTTCGAGATCGCCTGGTTCGCCGAGCCGCTCTTCAAGACCG
GGGACTACCCCGCGGCCATGAGGGAATACATTGCCTCCAAGCACCGACGG
GGGCTTTCCAGCTCGGCCCTGCCGCGCCTCACCGAGGCCGAAAGGAGGCT
GCTCAAGGGCACGGTCGACTTCTGCGCGCTCAACCACTTCACCACTAGGT
TCGTGATGCACGAGCAGCTGGCCGGCAGCCGCTACGACTCGGACAGGGAC
ATCCAGTTTCTGCAGGACATCACCCGCCTGAGCTCCCCCACGCGCCTGGC
TGTGATTCCCTGGGGGGTGCGCAAGCTGCTGCGGTGGGTCCGGAGGAACT
ACGGCGACATGGACATTTACATCACCGCCAGTGGCATCGACGACCAGGCT
CTGGAGGATGACCGGCTCCGGAAGTACTACCTAGGGAAGTACCTTCAGGA
GGTGCTGAAAGCATACCTGATTGATAAAGTCAGAATCAAAGGCTATTATG
CATTCAAACTGGCTGAAGAGAAATCTAAACCCAGATTTGGATTCTTCACA
TCTGATTTTAAAGCTAAATCCTCAATACAATTTTACAACAAAGTGATCAG
CAGCAGGGGCTTCCCTTTTGAGAACAGTAGTTCTAGATGCAGTCAGACCC
AAGAAAATACAGAGTGCACTGTCTGCTTATTCCTTGTGCAGAAGAAACCA
CTGATATTCCTGGGTTGTTGCTTCTTCTCCACCCTGGTTCTACTCTTATC
AATTGCCATTTTTCAAAGGCAGAAGAGAAGAAAGTTTTGGAAAGCAAAAA
ACTTACAACACATACCATTAAAGAAAGGCAAGAGAGTTGTTAGCGCGGCC
GCA

Amino Acid Sequence

MKPGCAAGSPGNEWIFFSTDEITTRYRNTMSNGGLQRSVILSALILLRAV
TGFSGDGRAIWSKNPNFTPVNESQLFLYDTFPKNFFWGIGTGALQVEGSW
KKDGKGPSIWDHFIHTHLKNVSSTNGSSDSYIFLEKDLSALDFIGVSFYQ
FSISWPRLFPDGIVTVANAKGLQYYSTLLDALVLRNIEPIVTLYHWDLPL
ALQEKYGGWKNDTIIDIFNDYATYCFQMFGDRVKYWITIHNPYLVAWHGY
GTGMHAPGEKGNLAAVYTVGHNLIKAHSKVWHNYNTHFRPHQKGWLSITL
GSHWIEPNRSENTMDIFKCQQSMVSVLGWFANPIHGDGDYPEGMRKKLFS
VLPIFSEAEKHEMRGTADFFAFSFGPNNFKPLNTMAKMGQNVSLNLREAL
NWIKLEYNNPRILIAENGWFTDSRVKTEDTTAIYMMKNFLSQVLQAIRLD
EIRVFGYTAWSLLDGFEWQDAYTIRRGLFYVDFNSKQKERKPKSSAHYYK
QIIRENGFSLKESTPDVQGQFPCDFSWGVTESVLKPESVASSPQFSDPHL
YVWNATGNRLLHRVEGVRLKTRPAQCTDFVNIKKQLEMLARMKVTHYRFA
LDWASVLPTGNLSAVNRQALRYYRCVVSEGLKLGISAMVTLYYPTHAHLG
LPEPLLHADGWLNPSTAEAFQAYAGLCFQELGDLVKLWITINEPNRLSDI
YNRSGNDTYGAAHNLLVAHALAWRLYDRQFRPSQRGAVSLSLHADWAEPA
NPYADSHWRAAERFLQFEIAWFAEPLFKTGDYPAAMREYIASKHRRGLSS
SALPRLTEAERRLLKGTVDFCALNHFTTRFVMHEQLAGSRYDSDRDIQFL
QDITRLSSPTRLAVIPWGVRKLLRWVRRNYGDMDIYITASGIDDQALEDD
RLRKYYLGKYLQEVLKAYLIDKVRIKGYYAFKLAEEKSKPRFGFFTSDFK
AKSSIQFYNKVISSRGFPFENSSSRCSQTQENTECTVCLFLVQKKPLIFL
GCCFFSTLVLLLSIAIFQRQKRRKFWKAKNLQHIPLKKGKRVVSAAAMVS
KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGK
LPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYI
MADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLS
TQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31609
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee