cDNA Clone BCLAF1

• Plasmid: pcDNA5-FRT/TO-HA2 BCLAF1
• Parent Plasmid: pcDNA5 FRT/TO HA2
• Expressed: HA BCLAF1
• Genbank: NM_014739.2 GI:117938252
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCAATGGGTCGCTCCAATTCTAGATCACATTCTTCAAGGTCAAA
GTCTAGATCACAGTCTAGTTCTCGATCAAGATCAAGATCTCATTCTAGAA
AGAAGCGATACAGTTCTAGGTCTCGTTCCAGAACATATTCAAGGTCTCGT
AGTAGAGATCGTATGTATTCTAGAGATTATCGTCGCGATTACAGAAATAA
TAGAGGAATGAGACGACCTTATGGGTACAGAGGAAGGGGTAGAGGGTATT
ATCAAGGAGGAGGAGGTAGATATCATCGAGGTGGTTATAGACCTGTCTGG
AATAGAAGGCACTCTAGGAGTCCTAGACGAGGTCGTTCACGTTCCAGGAG
TCCAAAAAGAAGATCCGTTTCTTCTCAAAGATCCAGAAGCAGATCTCGCC
GGTCATATAGATCTTCTAGGTCTCCAAGATCATCCTCTTCTCGTTCTTCA
TCCCCATATAGCAAATCTCCTGTTTCTAAAAGACGAGGGTCTCAGGAAAA
ACAAACCAAAAAAGCTGAAGGGGAACCCCAAGAAGAGAGTCCGTTGAAAA
GTAAATCACAGGAGGAACCGAAAGATACATTTGAACATGACCCATCTGAG
TCTATCGATGAATTTAATAAGTCATCAGCCACATCCGGTGATATTTGGCC
TGGCCTTTCAGCTTATGATAATAGTCCTAGATCACCCCATAGTCCTTCAC
CTATTGCTACACCACCTAGTCAGAGTTCATCTTGCTCTGATGCTCCCATG
CTCAGTACAGTTCACTCTGCAAAAAATACTCCTTCTCAGCATTCACATTC
CATTCAGCATAGTCCTGAAAGGTCTGGGTCTGGTTCTGTTGGAAATGGAT
CTAGTCGATACAGTCCTTCTCAGAATAGTCCAATTCATCACATCCCTTCA
CGAAGAAGTCCTGCAAAGACAATCGCACCACAGAATGCTCCAAGAGATGA
GTCTAGGGGCCGTTCCTCGTTTTATCCTGATGGTGGAGATCAGGAAACTG
CAAAGACTGGGAAGTTCTTAAAAAGGTTCACAGATGAAGAGTCTAGAGTA
TTCCTGCTTGATAGGGGTAATACCAGGGATAAAGAGGCTTCAAAAGAGAA
AGGATCAGAGAAAGGGAGGGCAGAGGGAGAATGGGAAGATCAGGAAGCTC
TAGATTACTTCAGTGATAAAGAGTCTGGAAAACAAAAGTTTAATGATTCA
GAAGGGGATGACACAGAGGAGACAGAGGATTATAGACAGTTCAGGAAGTC
AGTCCTCGCAGATCAGGGTAAAAGTTTTGCTACTGCATCTCACCGGAATA
CTGAGGAGGAAGGACTCAAGTACAAGTCCAAAGTTTCACTGAAAGGCAAT
AGAGAAAGTGATGGATTTAGAGAAGAAAAAAATTATAAACTTAAAGAGAC
TGGATATGTAGTGGAAAGGCCTAGCACTACAAAAGATAAGCACAAAGAAG
AAGACAAAAATTCTGAAAGAATAACAGTAAAGAAAGAAACTCAGTCACCT
GAGCAGGTAAAGTCTGAAAAGCTCAAAGACCTCTTTGATTACAGTCCCCC
TCTACACAAGAATCTGGATGCACGAGAAAAGTCTACCTTCAGAGAGGAAA
GCCCACTTAGGATCAAAATGATAGCGAGTGATTCTCACCGTCCTGAAGTC
AAACTCAAAATGGCACCTGTTCCTCTTGATGATTCTAACAGACCTGCTTC
CTTGACTAAAGACAGGCTGCTTGCTAGTACACTTGTCCATTCTGTCAAGA
AGGAGCAAGAATTCCGATCCATCTTTGACCACATTAAGTTGCCACAGGCC
AGCAAAAGCACTTCAGAGTCATTTATTCAACACATTGTGTCCTTGGTTCA
TCATGTTAAAGAGCAATACTTCAAGTCAGCTGCAATGACCCTAAACGAGC
GGTTCACTTCGTATCAGAAAGCCACTGAAGAACATAGTACTCGGCAAAAG
AGCCCTGAAATACACAGGAGAATTGACATCTCACCAAGTACCCTGAGGAA
GCATACCCGTTTAGCAGGGGAAGAGAGAGTTTTTAAAGAAGAAAATCAAA
AGGGAGATAAAAAATTAAGGTGTGACTCTGCTGACCTTCGGCATGACATT
GATCGCCGTAGAAAAGAAAGAAGTAAAGAACGGGGAGATTCCAAGGGCTC
CAGGGAATCCAGTGGATCAAGAAAGCAGGAAAAAACTCCAAAAGATTACA
AGGAATACAAATCTTACAAAGATGACAGTAAACATAAAAGAGAGCAAGAT
CATTCTCGATCTTCATCCTCTTCAGCATCACCTTCTTCTCCCAGTTCTCG
AGAAGAAAAGGAGAGTAAGAAGGAAAGAGAAGAAGAATTTAAAACTCACC
ATGAAATGAAAGAATACTCAGGCTTTGCAGGAGTTAGCCGACCACGAGGA
ACCTTTTTTCGAATTAGAGGCAGAGGAAGAGCCAGAGGAGTTTTTGCTGG
GACAAATACTGGTCCAAACAACTCAAATACTACTTTTCAAAAGAGACCGA
AGGAAGAGGAATGGGATCCAGAATATACCCCAAAGAGCAAGAAGTACTTC
TTGCATGACGACAGAGATGATGGTGTGGATTATTGGGCCAAAAGAGGAAG
AGGTCGTGGTACTTTTCAACGTGGCAGAGGGCGCTTTAACTTCAAAAAAT
CAGGTAGCAGTCCTAAATGGACTCATGACAAATACCAAGGGGATGGGATT
GTTGAAGATGAAGAAGAGACCATGGAAAATAATGAAGAAAAGAAGGACAG
ACGCAAGGAAGAAAAGGAATAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMGRSNSRSHSSRSKSRSQSSSRSRSRS
HSRKKRYSSRSRSRTYSRSRSRDRMYSRDYRRDYRNNRGMRRPYGYRGRG
RGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRS
RSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEES
PLKSKSQEEPKDTFEHDPSESIDEFNKSSATSGDIWPGLSAYDNSPRSPH
SPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSV
GNGSSRYSPSQNSPIHHIPSRRSPAKTIAPQNAPRDESRGRSSFYPDGGD
QETAKTGKFLKRFTDEESRVFLLDRGNTRDKEASKEKGSEKGRAEGEWED
QEALDYFSDKESGKQKFNDSEGDDTEETEDYRQFRKSVLADQGKSFATAS
HRNTEEEGLKYKSKVSLKGNRESDGFREEKNYKLKETGYVVERPSTTKDK
HKEEDKNSERITVKKETQSPEQVKSEKLKDLFDYSPPLHKNLDAREKSTF
REESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVH
SVKKEQEFRSIFDHIKLPQASKSTSESFIQHIVSLVHHVKEQYFKSAAMT
LNERFTSYQKATEEHSTRQKSPEIHRRIDISPSTLRKHTRLAGEERVFKE
ENQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTP
KDYKEYKSYKDDSKHKREQDHSRSSSSSASPSSPSSREEKESKKEREEEF
KTHHEMKEYSGFAGVSRPRGTFFRIRGRGRARGVFAGTNTGPNNSNTTFQ
KRPKEEEWDPEYTPKSKKYFLHDDRDDGVDYWAKRGRGRGTFQRGRGRFN
FKKSGSSPKWTHDKYQGDGIVEDEEETMENNEEKKDRRKEEKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31599
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee