cDNA Clone Lipin1

• Plasmid: pGEX6P-LIPIN1
• Parent Plasmid: pGEX6P2
• Expressed: GST-LIPIN1
• Genbank: NM_145693.1 GI:22027647
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGAATTACGTGGGGCAGTTAGCCGGCCAGGTGTTTGTCACCGT
GAAGGAGCTCTACAAGGGGCTGAATCCCGCCACACTCTCAGGGTGCATTG
ACATCATTGTCATCCGCCAGCCCAATGGAAACCTCCAATGCTCCCCTTTC
CACGTCCGCTTTGGGAAGATGGGGGTCCTGCGCTCCCGAGAGAAAGTGGT
TGACATAGAAATCAATGGGGAATCTGTGGATTTGCATATGAAATTGGGAG
ATAATGGAGAAGCATTTTTTGTTCAAGAAACAGATAATGATCAGGAAGTT
ATCCCTATGCACCTGGCCACCTCCCCCATCCTGTCAGAAGGAGCTTCGAG
AATGGAATGCCAGCTGAAAAGGGGCTCTGTGGACAGGATGAGAGGCCTGG
ACCCCAGCACGCCAGCCCAAGTGATCGCTCCCAGCGAGACGCCGTCAAGC
AGCTCTGTAGTAAAGAAGAGAAGAAAAAGGAGGAGAAAGTCACAGCTGGA
CAGCCTGAAGAGAGATGACAACATGAACACATCTGAGGATGAGGACATGT
TCCCCATCGAGATGAGCTCGGATGAGGCCATGGAGCTGCTGGAGAGCAGC
AGAACTCTTCCTAATGATATACCTCCATTCCAAGATGATATTCCTGAGGA
AAACCTCTCCCTGGCTGTGATTTACCCTCAGTCAGCCTCATACCCTAATT
CGGATAGAGAGTGGTCACCCACTCCCAGTCCTTCCGGTTCCCGACCTTCA
ACACCTAAAAGTGATTCAGAATTGGTCAGCAAGTCCACGGAAAGGACAGG
GCAGAAGAACCCAGAAATGCTTTGGCTGTGGGGAGAGCTGCCGCAGGCTG
CTAAGTCTTCTTCTCCACACAAGATGAAAGAGTCCAGCCCATTGAGCAGT
AGAAAAATTTGTGATAAAAGTCACTTTCAGGCCATTCACAGCGAATCTTC
AGACACTTTTAGTGACCAATCGCCAACTCTGGTCGGTGGGGCACTTTTGG
ACCAGAACAAGCCTCAGACAGAAATGCAGTTTGTGAATGAAGAAGACCTG
GAGACCTTAGGAGCAGCAGCGCCACTCTTGCCCATGATCGAGGAGCTCAA
ACCCCCCTCTGCCAGTGTAGTCCAGACAGCAAACAAGACGGATTCTCCTT
CCAGGAAAAGAGATAAACGAAGCCGACATCTTGGTGCTGACGGCGTCTAC
TTGGATGACCTCACAGACATGGATCCTGAAGTGGCGGCCCTGTATTTTCC
CAAAAACGGAGATCCTTCCGGACTCGCAAAACATGCAAGCGACAACGGAG
CCCGGTCAGCCAACCAGTCCCCGCAGTCGGTGGGCAGCTCGGGCGTGGAC
AGTGGCGTGGAGAGCACCTCGGACGGGCTGAGGGACCTCCCTTCCATCGC
CATCTCCCTCTGCGGGGGCCTCAGCGACCACCGGGAGATCACGAAAGATG
CATTCCTGGAGCAAGCTGTGTCATATCAACAGTTTGTGGACAACCCCGCT
ATTATCGATGACCCCAATCTCGTGGTAAAGATTGGGAGTAAATATTATAA
CTGGACAACAGCAGCACCCCTCCTCCTGGCAATGCAGGCCTTCCAGAAAC
CTTTGCCAAAGGCCACTGTGGAATCTATCATGAGGGATAAAATGCCCAAA
AAGGGAGGAAGATGGTGGTTTTCATGGAGGGGAAGAAACACCACAATCAA
GGAGGAAAGTAAGCCAGAGCAGTGCTTGGCTGGCAAGGCCCATAGCACCG
GAGAGCAACCGCCGCAGCTCAGCTTGGCCACCAGGGTAAAGCATGAATCA
TCCTCCAGTGATGAGGAGCGCGCAGCTGCCAAGCCATCAAACGCAGGCCA
CCTCCCTCTTCTGCCTAATGTCAGCTACAAGAAGACTCTCCGGCTGACTT
CCGAGCAGCTTAAAAGCTTGAAGTTGAAGAATGGCCCCAACGACGTGGTT
TTCAGTGTCACCACGCAGTACCAAGGCACGTGCCGCTGTGAGGGCACCAT
CTATCTGTGGAACTGGGATGATAAAGTCATCATTTCTGATATTGATGGGA
CAATTACCAGATCAGATACTCTTGGCCACATTTTGCCCACCCTTGGGAAG
GATTGGACCCATCAGGGCATCGCTAAGCTGTACCATAAAGTGAGCCAGAA
TGGATATAAATTTCTCTACTGTTCTGCCCGTGCCATCGGGATGGCGGACA
TGACGCGGGGCTACCTGCACTGGGTCAACGAGAGGGGCACGGTGCTGCCC
CAGGGGCCCCTGCTGCTGAGTCCCAGCAGCCTCTTCTCTGCCCTGCACAG
AGAAGTGATTGAAAAGAAGCCAGAAAAGTTTAAAGTCCAGTGTTTGACAG
ACATCAAAAACCTGTTTTTCCCCAACACAGAACCCTTTTATGCTGCTTTT
GGAAACCGACCAGCTGATGTGTATTCATACAAGCAAGTAGGAGTGTCTTT
GAATAGAATATTTACCGTCAACCCTAAAGGAGAGCTGGTACAGGAACATG
CAAAGACCAACATCTCTTCGTATGTGAGACTCTGTGAAGTAGTCGACCAC
GTTTTCCCGTTGCTGAAAAGAAGCCATTCTTCAGACTTTCCCTGTTCGGA
TACCTTCAGTAACTTCACCTTTTGGAGAGAGCCACTGCCACCTTTTGAAA
ACCAGGACATTCATTCTGCCTCAGCGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNYVGQLAGQVFVTVKELY
KGLNPATLSGCIDIIVIRQPNGNLQCSPFHVRFGKMGVLRSREKVVDIEI
NGESVDLHMKLGDNGEAFFVQETDNDQEVIPMHLATSPILSEGASRMECQ
LKRGSVDRMRGLDPSTPAQVIAPSETPSSSSVVKKRRKRRRKSQLDSLKR
DDNMNTSEDEDMFPIEMSSDEAMELLESSRTLPNDIPPFQDDIPEENLSL
AVIYPQSASYPNSDREWSPTPSPSGSRPSTPKSDSELVSKSTERTGQKNP
EMLWLWGELPQAAKSSSPHKMKESSPLSSRKICDKSHFQAIHSESSDTFS
DQSPTLVGGALLDQNKPQTEMQFVNEEDLETLGAAAPLLPMIEELKPPSA
SVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGD
PSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRDLPSIAISLC
GGLSDHREITKDAFLEQAVSYQQFVDNPAIIDDPNLVVKIGSKYYNWTTA
APLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGRNTTIKEESK
PEQCLAGKAHSTGEQPPQLSLATRVKHESSSSDEERAAAKPSNAGHLPLL
PNVSYKKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWN
WDDKVIISDIDGTITRSDTLGHILPTLGKDWTHQGIAKLYHKVSQNGYKF
LYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIE
KKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIF
TVNPKGELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSSDFPCSDTFSN
FTFWREPLPPFENQDIHSASA*
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31545
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 125484 Daltons