cDNA Clone IRS2 (mouse)

• Plasmid: pcDNA5-FRT/TO-GFP IRS2 (mouse)
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-IRS2 (mouse)
• Genbank: NM_001081212.1 GI:124487072
• Tag: GFP
• Species: Mouse

Sequence of Insert

GGATCCATGGCTAGCGCGCCCCTGCCTGGGCCCCCCGCGTCGGCGGGCGG
GGACGGCCCGAACCTCAATAACAACAACAACAACAACAACCACAGCGTGC
GCAAGTGCGGCTACCTGCGCAAGCAGAAGCACGGCCACAAGCGCTTTTTC
GTGTTGCGCGGCCCCGGCACGGGCGGCGACGAGGCATCCGCGGCTGGGGG
GTCGCCGCCGCAGCCTCCGCGGCTGGAGTACTACGAGAGCGAGAAGAAGT
GGAGGAGCAAGGCGGGCGCGCCGAAGCGAGTGATCGCGCTCGACTGCTGT
CTGAACATCAACAAGCGCGCGGACGCCAAGCACAAGTACCTGATCGCCCT
CTACACCAAGGACGAGTACTTCGCTGTAGCGGCGGAGAACGAGCAGGAGC
AGGAGGGCTGGTACCGCGCACTCACCGACTTGGTCAGCGAAGGCCGCTCT
GGCGAGGGGGGCTCGGGCACCACCGGAGGCTCTTGCAGCGCCTCTCTCCC
GGGCGTCCTGGGCGGCTCAGCGGGCGCCGCTGGCTGCGATGACAACTACG
GGCTCGTGACACCCGCCACGGCCGTCTACCGCGAGGTGTGGCAGGTGAAC
CTGAAACCTAAGGGACTGGGCCAGAGCAAGAACCTGACTGGTGTATACCG
CCTATGCCTGTCTGCGCGCACCATCGGCTTCGTGAAGCTCAATTGCGAAC
AGCCGTCGGTGACGCTGCAGCTTATGAACATTCGCCGCTGCGGCCACTCG
GACAGCTTCTTCTTCATCGAGGTGGGCCGTTCGGCCGTCACCGGTCCCGG
GGAGCTGTGGATGCAAGCCGACGACTCGGTGGTGGCGCAGAACATCCATG
AGACCATCCTAGAAGCTATGAAGGCACTCAAAGAGCTCTTCGAGTTCCGG
CCTCGCAGCAAGAGTCAGTCGTCCGGGTCGTCAGCCACGCATCCCATCAG
CGTGCCGGGCGCGCGCCGCCACCACCACCTAGTCAACCTACCCCCTAGCC
AGACCGGCCTGGTGCGCCGCTCGCGCACTGACAGCCTGGCGGCCACCCCC
CCAGCAGCCAAGTGCACTTCGTGCCGGGTTCGTACGGCCAGCGAGGGCGA
CGGCGGCGCGGCAGGCGGGGCCGGGACGGCAGGAGGCAGGCCGATGTCGG
TGGCAGGGAGCCCCCTGAGTCCCGGGCCGGTGCGCGCGCCCCTTAGCCGC
TCGCACACCCTGAGCGCCGGCTGCGGAGGCCGCCCGAGCAAAGTGACTCT
GGCGCCGGCAGGGGGAGCCCTGCAACACAGCCGCTCCATGTCCATGCCCG
TGGCGCACTCACCTCCTGCAGCCACCAGCCCAGGCAGCCTGTCCTCCAGC
AGTGGGCACGGCTCGGGCTCCTACCCGCTGCCACCTGGCTCCCACCCGCA
CCTGCCTCATCCACTGCATCACCCCCAAGGCCAGCGTCCGTCCAGCGGTA
GTGCCTCCGCCTCGGGCTCCCCCAGCGACCCGGGTTTCATGTCCCTTGAC
GAGTATGGCTCCAGCCCTGGCGACCTGAGAGCCTTCAGTAGCCACAGGAG
CAACACACCCGAGTCAATAGCGGAGACCCCGCCAGCCAGAGATGGCAGTG
GGGGCGAACTCTATGGGTACATGAGCATGGATAGACCCCTGAGCCACTGT
GGCCGCCCTTACCGTAGGGTCTCAGGGGATGGGGCCCAGGACCTGGATAG
AGGACTGAGGAAGAGGACTTATTCCCTAACCACGCCTGCCAGGCAGCGGC
AGGTACCTCAGCCTTCCTCTGCCTCTCTAGATGAATACACTCTCATGAGG
GCCACCTTCTCTGGTAGTTCAGGTCGCCTCTGCCCATCCTTCCCTGCGTC
CTCTCCCAAAGTGGCCTACAACCCTTACCCAGAGGACTATGGAGACATTG
AGATTGGTTCTCACAAGAGTTCCAGCAGTAACCTGGGGGCAGATGATGGC
TACATGCCCATGACCCCTGGGGCAGCCCTTAGGAGTGGTGGTCCCAATAG
CTGCAAGAGCGATGACTACATGCCCATGAGCCCCACAAGCGTGTCTGCTC
CCAAGCAGATCCTGCAGCCACGCTTGGCAGCGGCCTTGCCCCCTTCCGGA
GCAGCCGTGCCAGCACCCCCTTCAGGGGTGGGCAGGACCTTCCCAGTAAA
CGGAGGTGGCTACAAAGCCAGCTCCCCAGCGGAGAGCTCCCCAGAAGACA
GTGGGTACATGCGAATGTGGTGTGGCTCCAAGCTGTCTATGGAGAACCCA
GACCCTAAGCTACTCCCCAACGGGGACTACCTCAACATGTCCCCCAGCGA
GGCAGGCACTGCAGGGACCCCACCTGACTTCTCAGCAGCTTTGCGTGGAG
GCAGTGAAGGCCTCAAAGGCATCCCGGGCCACTGCTACAGCTCTTTGCCC
CGCTCTTATAAGGCTCCCTGTTCCTGCAGCGGAGACAATGACCAGTATGT
GCTCATGAGCTCCCCTGTGGGCCGGATCTTGGAAGAGGAGAGACTGGAGC
CCCAGGCCACCCCAGGGGCTGGCACCTTTGGGGCAGCTGGTGGTAGTCAT
ACCCAGCCTCATCACTCAGCAGTGCCTTCCTCCATGAGGCCGAGTGCCAT
CGGTGGCCGCCCTGAGGGCTTCCTGGGCCAGCGATGTCGGGCAGTGCGGC
CTACACGCCTATCGCTAGAGGGACTGCAGACCCTTCCCAGCATGCAAGAG
TACCCTCTACCCACAGAGCCCAAGAGCCCTGGCGAGTACATCAACATTGA
CTTTGGTGAGGCAGGTACCCGTCTGTCTCCGCCTGCCCCCCCACTACTGG
CATCCGCGGCCTCATCTTCTTCACTGCTCTCAGCTAGTAGTCCTGCTTCA
TCCCTGGGTTCAGGAACCCCAGGCACCAGCAGCGACAGCCGGCAGCGCTC
TCCACTCTCTGACTATATGAACCTGGACTTCAGTTCTCCCAAGTCCCCCA
AGCCTAGCACCCGCAGTGGGGACACAGTAGGCTCCATGGATGGCCTTCTC
TCTCCAGAGGCTTCATCCCCATACCCACCACTGCCCCCACGTCCTTCCAC
TTCCCCTTCCTCCTTACAGCAGCCTCTGCCACCTGCCCCGGGAGACCTAT
ACCGCCTGCCTCCAGCATCAGCTGCCACTTCCCAGGGTCCCACTGCTGGC
TCCTCAATGTCCTCCGAGCCTGGGGATAATGGTGACTATACCGAGATGGC
CTTTGGTGTGGCTGCAACCCCGCCACAACCTATCGTGGCACCTCCAAAGC
CAGAAGGTGCCCGAGTGGCCAGTCCCACATCGGGCTTGAAGCGGCTAAGT
CTCATGGATCAGGTATCTGGGGTGGAGGCTTTCCTTCAAGTCAGCCAGCC
CCCTGACCCCCACCGGGGTGCTAAGGTCATCCGTGCAGACCCACAGGGGG
GACGTCGTCGCCACAGTTCAGAGACCTTTTCCTCTACCACCACCGTCACC
CCAGTGTCCCCATCCTTTGCCCACAATTCCAAGCGCCACAATTCGGCCTC
TGTGGAAAATGTCTCACTCAGGAAAAGCAGTGAAGGCAGCAGTACCCTGG
GAGGAGGTGATGAGCCGCCCACATCCCCAGGACAGGCACAGCCCTTGGTG
GCTGTGCCCCCAGTGCCACAGGCTAGGCCGTGGAACCCCGGTCAGCCCGG
AGCTTTGATTGGCTGTCCTGGAGGCAGCAGTTCTCCCATGCGCAGAGAGA
CCTCCGTGGGTTTCCAGAACGGCCTCAACTATATCGCCATCGATGTGAGA
GGCGAGCAGGGGTCCTTGGCGCAGTCTCAGCCGCAGCCAGGAGACAAGAA
CTCCTGGAGCCGGACCCGTAGCCTTGGGGGGCTCCTCGGCACCGTCGGAG
GCTCTGGCGCCAGCGGAGTGTGTGGGGGTCCAGGCACTGGAGCTTTGCCC
TCTGCCAGCACCTATGCAAGCATCGACTTCCTGTCCCATCACTTGAAGGA
AGCCACAGTCGTGAAAGAGTGACTCGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMASAPL
PGPPASAGGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGTG
GDEASAAGGSPPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRAD
AKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLVSEGRSGEGGSGTT
GGSCSASLPGVLGGSAGAAGCDDNYGLVTPATAVYREVWQVNLKPKGLGQ
SKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEV
GRSAVTGPGELWMQADDSVVAQNIHETILEAMKALKELFEFRPRSKSQSS
GSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPAAKCTSC
RVRTASEGDGGAAGGAGTAGGRPMSVAGSPLSPGPVRAPLSRSHTLSAGC
GGRPSKVTLAPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSY
PLPPGSHPHLPHPLHHPQGQRPSSGSASASGSPSDPGFMSLDEYGSSPGD
LRAFSSHRSNTPESIAETPPARDGSGGELYGYMSMDRPLSHCGRPYRRVS
GDGAQDLDRGLRKRTYSLTTPARQRQVPQPSSASLDEYTLMRATFSGSSG
RLCPSFPASSPKVAYNPYPEDYGDIEIGSHKSSSSNLGADDGYMPMTPGA
ALRSGGPNSCKSDDYMPMSPTSVSAPKQILQPRLAAALPPSGAAVPAPPS
GVGRTFPVNGGGYKASSPAESSPEDSGYMRMWCGSKLSMENPDPKLLPNG
DYLNMSPSEAGTAGTPPDFSAALRGGSEGLKGIPGHCYSSLPRSYKAPCS
CSGDNDQYVLMSSPVGRILEEERLEPQATPGAGTFGAAGGSHTQPHHSAV
PSSMRPSAIGGRPEGFLGQRCRAVRPTRLSLEGLQTLPSMQEYPLPTEPK
SPGEYINIDFGEAGTRLSPPAPPLLASAASSSSLLSASSPASSLGSGTPG
TSSDSRQRSPLSDYMNLDFSSPKSPKPSTRSGDTVGSMDGLLSPEASSPY
PPLPPRPSTSPSSLQQPLPPAPGDLYRLPPASAATSQGPTAGSSMSSEPG
DNGDYTEMAFGVAATPPQPIVAPPKPEGARVASPTSGLKRLSLMDQVSGV
EAFLQVSQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAH
NSKRHNSASVENVSLRKSSEGSSTLGGGDEPPTSPGQAQPLVAVPPVPQA
RPWNPGQPGALIGCPGGSSSPMRRETSVGFQNGLNYIAIDVRGEQGSLAQ
SQPQPGDKNSWSRTRSLGGLLGTVGGSGASGVCGGPGTGALPSASTYASI
DFLSHHLKEATVVKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31523
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee